Protein Overview: PPX1

General Information:
Name(s) found:	PPX1 / YHR201C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|730369\|sp\|P38698.1\|PPX1_YEAST RecName: Full=Exopolyphosphatase; Short=ExopolyPase; AltName: Full=Metaphosphatase [NCBI NR] gi\|6321995\|ref\|NP_012071.1\| Exopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix; Ppx1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S46691 exopolyphosphatase (EC 3.6.1.11) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|458943\|gb\|AAB68368.1\| Ppx1p: Cytosolic exopolyphosphatase [Saccharomyces cerevisiae] [NCBI NR] gi\|162330022\|pdb\|2QB8\|B Chain B, Saccharomyces Cerevisiae Cytosolic Exopolyphosphatase, Atp Complex [NCBI NR] gi\|162330021\|pdb\|2QB8\|A Chain A, Saccharomyces Cerevisiae Cytosolic Exopolyphosphatase, Atp Complex [NCBI NR] gi\|162330020\|pdb\|2QB7\|B Chain B, Saccharomyces Cerevisiae Cytosolic Exopolyphosphatase, Phosphate Complex [NCBI NR] gi\|162330019\|pdb\|2QB7\|A Chain A, Saccharomyces Cerevisiae Cytosolic Exopolyphosphatase, Phosphate Complex [NCBI NR] gi\|162330018\|pdb\|2QB6\|B Chain B, Saccharomyces Cerevisiae Cytosolic Exopolyphosphatase, Sulfate Complex [NCBI NR] gi\|162330017\|pdb\|2QB6\|A Chain A, Saccharomyces Cerevisiae Cytosolic Exopolyphosphatase, Sulfate Complex [NCBI NR] Exopolyphosphatase OS=Saccharomyces cerevisiae GN=PPX1 PE=1 SV=1 [Swiss-Prot] PPX1 Exopolyphosphatase [mips] Exopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix [SGD] PPX1, Chr VIII from 499946-501139, reverse complement [yeast_orfs2] Chr VIII from 499946-501139, reverse complement [yeastorf3.fasta] Chr VIII from 501139-499946, reverse complement [yeastorf5.fasta] (P38698) Exopolyphosphatase (EC 3.6.1.11) (ExopolyPase) (Metaphosphatase) [4932.FASTACCERV.txt] PPX1 SGDID:S0001244, Chr VIII from 501141-499948, reverse complement, Verified ORF [yeastflysequest.fasta] PPX1 SGDID:S0001244, Chr VIII from 501142-499949, reverse complement, Verified ORF [10112005cervSGD.fasta] Exopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix [Saccharomyces cerevisiae]gi\|730369\|sp\|P38698\|PPX1_YEAST Exopolyphosphatase (ExopolyPase) (Metaphosphatase [nrdb_11152004_Chlamydomonas] PPX1 SGDID:S000001244, Chr VIII from 501143-499950, reverse complement, Verified ORF, "Exopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Exopolyphosphatase (ExopolyPase) (Metaphosphatase) [nr-271106-contam.fasta] Exopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix; Ppx1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ppx1p: Cytosolic exopolyphosphatase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Exopolyphosphatase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PPX1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PPX1 SGDID:S000001244, Chr VIII from 501143-499950, Genome Release 64-1-1, reverse complement, Verified ORF, "Exopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix" [yeast-030211-contam.fasta] PPX1 SGDID:S000001244, Chr VIII from 501143-499950, Genome Release 64-2-1, reverse complement, Verified ORF, "Exopolyphosphatase; hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix" [20141208_orf_trans.fasta] YHR201C SGDID:S000001244, Chr VIII from 501143-499950, Genome Release 64-1-1, reverse complement, Verified ORF, "Exopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	397 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] mitochondrial matrix [TAS] cytosol [TAS] plasma membrane [TAS]
Biological Process:	polyphosphate metabolic process [IDA]
Molecular Function:	exopolyphosphatase activity [IDA ][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSPLRKTVPE FLAHLKSLPI SKIASNDVLT ICVGNESADM DSIASAITYS YCQYIYNEGT  60
   61 YSEEKKKGSF IVPIIDIPRE DLSLRRDVMY VLEKLKIKEE ELFFIEDLKS LKQNVSQGTE 120
  121 LNSYLVDNND TPKNLKNYID NVVGIIDHHF DLQKHLDAEP RIVKVSGSCS SLVFNYWYEK 180
  181 LQGDREVVMN IAPLLMGAIL IDTSNMRRKV EESDKLAIER CQAVLSGAVN EVSAQGLEDS 240
  241 SEFYKEIKSR KNDIKGFSVS DILKKDYKQF NFQGKGHKGL EIGLSSIVKR MSWLFNEHGG 300
  301 EADFVNQCRR FQAERGLDVL VLLTSWRKAG DSHRELVILG DSNVVRELIE RVSDKLQLQL 360
  361 FGGNLDGGVA MFKQLNVEAT RKQVVPYLEE AYSNLEE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		PPX1 SEC7
View Details	Ho Y, et al. (2002)		AAC1 AAC3 ADE5,7 AHA1 ARC1 ARO1 ARP2 ATG11 ATG12 ATG17 CAR2 CPA2 CPR6 FET4 GCD11 GFA1 GUS1 IPP1 PPX1 PRB1 PTC7 REP1 REX2 RPN10 RPN11 RPN3 RPN5 RPN6 RPN7 RPT1 RPT3 SEC18 TCB1 TIF1, TIF2 TYR1 YHR020W YHR033W YNL208W

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	LOS1	PPX1	1	Unpublished Fields Lab Data
View Screen	UBC7	PPX1	1	Unpublished Fields Lab Data
View Screen	NAF1	PPX1	1	Hazbun TR, et al. (2003)
View Screen	MFB1	PPX1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PPX1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..251]	PSI-BLAST	56.522879	Manganese-dependent inorganic pyrophosphatase (family II)
2	View Details	[252..397]	PSI-BLAST	56.522879	Manganese-dependent inorganic pyrophosphatase (family II)

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
nuclease activity	2.22762374984422	bayes_pls_golite062009
hydrolase activity	2.11996205929773	bayes_pls_golite062009
catalytic activity	1.78154379547895	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	1.7679956422453	bayes_pls_golite062009
deoxyribonuclease activity	1.7238398802096	bayes_pls_golite062009
binding	1.52735252886759	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	1.48438369225803	bayes_pls_golite062009
exonuclease activity	1.4791620684568	bayes_pls_golite062009
single-stranded DNA specific exodeoxyribonuclease activity	1.10404503151762	bayes_pls_golite062009
exodeoxyribonuclease activity	0.808006273031307	bayes_pls_golite062009
exodeoxyribonuclease activity, producing 5'-phosphomonoesters	0.792120683260194	bayes_pls_golite062009
protein binding	0.332901114484316	bayes_pls_golite062009
inorganic diphosphatase activity	0.158887418739632	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2