Protein Overview: ADE6

General Information:
Name(s) found:	ADE6 / YGR061C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321498\|ref\|NP_011575.1\| Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; Ade6p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S64356 phosphoribosylformylglycinamidine synthase (EC 6.3.5.3) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1709924\|sp\|P38972.2\|PUR4_YEAST RecName: Full=Phosphoribosylformylglycinamidine synthase; Short=FGAM synthase; Short=FGAMS; AltName: Full=Formylglycinamide ribotide amidotransferase; Short=FGARAT; AltName: Full=Formylglycinamide ribotide synthetase [NCBI NR] gi\|1323079\|emb\|CAA97063.1\| ADE6 [Saccharomyces cerevisiae] [NCBI NR] Phosphoribosylformylglycinamidine synthase OS=Saccharomyces cerevisiae GN=ADE6 PE=1 SV=2 [Swiss-Prot] ADE6 5`-phosphoribosylformyl glycinamidine synthetase [mips] Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway [SGD] ADE6, Chr VII from 611883-615959, reverse complement [yeast_orfs2] Chr VII from 611883-615959, reverse complement [yeastorf3.fasta] Chr VII from 611890-615966, reverse complement [yeastorf4.fasta] Chr VII from 615966-611890, reverse complement [yeastorf5.fasta] (P38972) Phosphoribosylformylglycinamidine synthase (EC 6.3.5.3) (FGAM synthase) (FGAMS) (Formylglycinamide ribotide amidotransferase) (FGARAT) (Formylglycinamide ribotide synthetase) [4932.FASTACCERV.txt] ADE6 SGDID:S0003293, Chr VII from 615967-611891, reverse complement, Verified ORF [yeastflysequest.fasta] ADE6 SGDID:S0003293, Chr VII from 615969-611893, reverse complement, Verified ORF [10112005cervSGD.fasta] Ade6p [Saccharomyces cerevisiae]gi\|1323079\|emb\|CAA97063.1\| ADE6 [Saccharomyces cerevisiae]gi\|2118366\|pir\|\|S64356 phosphoribosylformylglycinamidine synthase (EC 6.3.5.3) - yeast (Saccharomyces cerevisiae)gi\|1709924\|sp\|P38972\|PUR4_YEAST Phosphoribosylfor [nrdb_11152004_Chlamydomonas] ADE6 SGDID:S000003293, Chr VII from 615969-611893, reverse complement, Verified ORF, "Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; Ade6p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Phosphoribosylformylglycinamidine synthase (FGAM synthase) (FGAMS) (Formylglycinamide ribotide amidotransferase) (FGARAT) (Formylglycinamide ribotide synthetase) [nr-271106-contam.fasta] ADE6 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Phosphoribosylformylglycinamidine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ADE6 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ADE6 SGDID:S000003293, Chr VII from 615965-611889, Genome Release 64-1-1, reverse complement, Verified ORF, "Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway" [yeast-030211-contam.fasta] ADE6 SGDID:S000003293, Chr VII from 615965-611889, Genome Release 64-2-1, reverse complement, Verified ORF, "Formylglycinamidine-ribonucleotide (FGAM)-synthetase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway" [20141208_orf_trans.fasta] YGR061C SGDID:S000003293, Chr VII from 615965-611889, Genome Release 64-1-1, reverse complement, Verified ORF, "Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1358 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	purine nucleotide biosynthetic process [IMP][TAS] 'de novo' IMP biosynthetic process [TAS]
Molecular Function:	phosphoribosylformylglycinamidine synthase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTDYILPGPK ALSQFRVDNL IKDINSYTNS TSVINELRSC YIHYVNGIAQ NLSEQDTKLL  60
   61 EVLLTYDSAL DIANDPLARQ LNDAVANNLP SSALGEDTYL IRVVPRSGTI SPWSSKATNI 120
  121 AHVCGLQDKV QRIERGLALL IKTVPGFPLL ENLNDISLKC VYDRMTQQLY LTEPPNTMSI 180
  181 FTHEEPKPLV HVPLTPKDTK QSPKDILSKA NTELGLALDS GEMEYLIHAF VETMKRDPTD 240
  241 VELFMFAQVN SEHCRHKIFN ADWTIDGIKQ QFTLFQMIRN THKLNPEYTI SAYSDNAAVL 300
  301 DSENDAFFFA PNSTTKEWTS TKERIPLLIK VETHNHPTAV SPFPGAATGS GGEIRDEGAT 360
  361 GRGSKTKCGL SGFSVSDLLI PGNEQPWELN IGKPYHIASA LDIMIEAPLG SAAFNNEFGR 420
  421 PCINGYFRTL TTKVLNHQGK EEIRGFHKPI MIAGGFGTVR PQFALKNTPI TPGSCLIVLG 480
  481 GQSMLIGLGG GAASSVASGE GSADLDFASV QRGNPEMERR CQQVIDACVA LGNNNPIQSI 540
  541 HDVGAGGLSN ALPELVHDND LGAKFDIRKV LSLEPGMSPM EIWCNESQER YVLGVSPQDL 600
  601 SIFEEICKRE RAPFAVVGHA TAEQKLIVED PLLKTTPIDL EMPILFGKPP KMSRETITEA 660
  661 LNLPEANLSE IPSLQDAIQR VLNLPSVGSK SFLITIGDRS VTGLIDRDQF VGPWQVPVAD 720
  721 VGVTGTSLGE TIISTGEAMA MGEKPVNALI SASASAKLSV AESLLNIFAA DVKSLNHIKL 780
  781 SANWMSPASH QGEGSKLYEA VQALGLDLCP ALGVAIPVGK DSMSMKMKWD DKEVTAPLSL 840
  841 NITAFAPVFN TSKTWTPLLN RNTDDSVLVL VDLSAKQETK SLGASALLQV YNQVGNKSPT 900
  901 VYDNAILKGF LESLIQLHQQ KEDIVLAYHD RSDGGLLITL LEMAFASRCG LEINIDGGDL 960
  961 ESQLTNLFNE ELGAVFQISA KNLSKFEKIL NENGVAKEYI SIVGKPSFQS QEIKIINSTT1020
 1021 NDVIYANSRS ELEQTWSKTS YEMQKLRDNP KTAEEEFASI TDDRDPGLQY ALTYNPADDM1080
 1081 KIGLELSSQR PKVAILREQG VNGQMEMAWC FQQAGFNSVD VTMTDLLEGR FHLDDFIGLA1140
 1141 ACGGFSYGDV LGAGAGWAKS VLYHEGVRSQ FSKFFNERQD TFAFGACNGC QFLSRLKDII1200
 1201 PGCENWPSFE RNVSEQYEAR VCMVQISQEK DNSSEESVFL NGMAGSKLPI AVAHGEGKAT1260
 1261 FSKSAEQLEK FEKDGLCCIR YVDNYGNVTE RFPFNPNGST NGIAGIKSPN GRVLAMMPHP1320
 1321 ERVCRLEANS WYPEGKYEEW GGYGPWIRLF RSARRWVG

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ADE6 BUL1 LDB19 RSP5 RUP1 UBP2 YHR131C
View Details	Gavin AC, et al. (2006)		ADE6 PET123 RSM23
View Details	Ho Y, et al. (2002)		ACS2 ADE3 ADE6 ADO1 ALA1 APE2 ARA1 BAT1 CDC10 CLU1 CYS3 DED81 DPS1 ERG10 ERG20 GCY1 GPH1 GRS1 HOM2 HYP2 ILS1 IMD3 KRS1 LSP1 MDH3 OLA1 PAB1 PFK2 PGM2 PMI40 PRP6 SAC6 SCP160 SOD1 SSZ1 THR4 TRR1 UBA1 YDL124W

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC23	ADE6	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	ADE6	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..251]	deduced	N/A	No confident structure predictions are available.
2	View Details	[252..463]	PSI-BLAST	66.39794	Aminoimidazole ribonucleotide synthetase (PurM) N-terminal domain; Aminoimidazole ribonucleotide synthetase (PurM) C-terminal domain
3	View Details	[464..484] [527..655]	PSI-BLAST	66.39794	Aminoimidazole ribonucleotide synthetase (PurM) N-terminal domain; Aminoimidazole ribonucleotide synthetase (PurM) C-terminal domain
4	View Details	[485..526]	PSI-BLAST	66.39794	Aminoimidazole ribonucleotide synthetase (PurM) N-terminal domain; Aminoimidazole ribonucleotide synthetase (PurM) C-terminal domain
5	View Details	[656..1062]	MSA	32.13098	View MSA. No confident structure predictions are available.
6	View Details	[1063..1358]	PSI-BLAST	10.69897	Cyclase subunit (or domain) of imidazoleglycerolphosphate synthase HisF; GAT subunit, HisH, (or domain) of imidazoleglycerolphosphate synthase HisF

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
phosphoribosylformylglycinamidine synthase activity	3.63602541423478	bayes_pls_golite062009
phosphoribosylformylglycinamidine cyclo-ligase activity	3.04322905277855	bayes_pls_golite062009
carbon-nitrogen ligase activity, with glutamine as amido-N-donor	2.83262699406158	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	2.47010130088456	bayes_pls_golite062009
cyclo-ligase activity	2.40623349460413	bayes_pls_golite062009
carbamoyl-phosphate synthase activity	1.74828881793189	bayes_pls_golite062009
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	1.74029540439985	bayes_pls_golite062009
binding	1.31749052462602	bayes_pls_golite062009
catalytic activity	1.23634637968745	bayes_pls_golite062009
ligase activity	1.09859780971947	bayes_pls_golite062009
transferase activity, transferring glycosyl groups	0.78656048097577	bayes_pls_golite062009
protein binding	0.288540446972424	bayes_pls_golite062009
nucleic acid binding	0.12569720972861	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.95

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2