Protein Overview: RPL30

General Information:
Name(s) found:	RPL30 / YGL030W [SGD]
Description(s) found: Found 33 descriptions. SHOW ALL	Protein component of the large (60S) ribosomal subunit, has similarity to rat L30 ribosomal protein; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript [SGD]
Organism:	Saccharomyces cerevisiae
Length:	105 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	negative regulation of nuclear mRNA splicing, via spliceosome [IMP] rRNA processing [IMP] negative regulation of translation [TAS ] translation [TAS]
Molecular Function:	structural constituent of ribosome [IDA][TAS]

Sequence:
Sequence: [PDR BLAST] [ProtParam]	1 11 21 31 41 51 \| \| \| \| \| \| 1 MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV LRKSELEYYA 60 61 MLSKTKVYYF QGGNNELGTA VGKLFRVGVV SILEAGDSDI LTTLA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		NHP6A PUF4 RPL11B RPL12B, RPL12A RPL15A RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL1A, RPL1B RPL20A RPL21A RPL24A RPL26A RPL29 RPL2A, RPL2B RPL3 RPL30 RPL32 RPL36A RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL5 RPL6A RPL8B RPL9A RPS11A, RPS11B RPS14A RPS16B, RPS16A RPS17B RPS1A RPS2 RPS22A RPS23B, RPS23A RPS26B RPS30B, RPS30A RPS5 RPS8B, RPS8A RPS9B YHI9
View Details	(MIPS) Mewes HW, et al. (2004)		RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL14B RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL20B RPL21A RPL21B RPL22A RPL22B RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27A RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL34B RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL4B RPL5 RPL6A RPL6B RPL7A RPL7B RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B
View Details	Gavin AC, et al. (2006)		RPL30 YNK1 YOR1
View Details	Qiu et al. (2008)		ASC1 BUD20 FYV12 MRPL50 NAT3 RKI1 RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL14B RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL20B RPL21A RPL21B RPL22A RPL22B RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27A RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL34B RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL4B RPL5 RPL6A RPL6B RPL7A RPL7B RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B RPS0A RPS0B RPS10A RPS10B RPS11A, RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16B, RPS16A RPS17A RPS17B RPS18B, RPS18A RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B RPS23B, RPS23A RPS24A, RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30B, RPS30A RPS31 RPS4B, RPS4A RPS5 RPS6A, RPS6B RPS7A RPS7B RPS8B, RPS8A RPS9A RPS9B RRP5 TEF1, TEF2 TSR1 VAR1 YML6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	DAD1	Dam1 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	MIH1	control 1	McCusker D, et al (2007)
View Run	MIH1	experimental2 - mih1-2	McCusker D, et al (2007)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	DAD1	Dam1 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	VAM3	sample: hx4	Hao Xu, et al. (2010)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..105]	PSI-BLAST	479.165202	Eukaryotic ribosomal protein L30 (L30e)

Functions predicted (by domain):

#

Gene Ontology predictions

1

Term	Confidence	Notes
structural constituent of ribosome	7.52323416912124	bayes_pls_golite062009
structural molecule activity	6.97581143389173	bayes_pls_golite062009
RNA binding	5.39762744650785	bayes_pls_golite062009
pre-mRNA 5'-splice site binding	5.13266824583271	bayes_pls_golite062009
	3.80269215693277	bayes_pls_golite062009
nucleic acid binding	2.83245292332884	bayes_pls_golite062009
mRNA binding	2.62944559104613	bayes_pls_golite062009
snoRNA binding	2.44542325037279	bayes_pls_golite062009
binding	1.91254208399282	bayes_pls_golite062009
protein binding	0.381324299701367	bayes_pls_golite062009
catalytic activity	0.264002643439785	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle