Protein Overview: GSY1

General Information:
Name(s) found:	GSY1 / YFR015C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|836770\|dbj\|BAA09254.1\| glycogen synthase isoform 1 [Saccharomyces cerevisiae] [NCBI NR] gi\|172870\|gb\|AAA88715.1\| glycogen synthase [Saccharomyces cerevisiae] [NCBI NR] gi\|14318537\|ref\|NP_116670.1\| Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; Gsy1p [Saccharomyces ce [NCBI NR] gi\|136753\|sp\|P23337.3\|GYS1_YEAST RecName: Full=Glycogen [starch] synthase isoform 1 [NCBI NR] pir\|\|A38326 glycogen(starch) synthase (EC 2.4.1.11) 1 - yeast (Saccharomyces cerevisiae) [NCBI NR] Glycogen [starch] synthase isoform 1 OS=Saccharomyces cerevisiae GN=GSY1 PE=1 SV=3 [Swiss-Prot] GSY1 UDP glucose--starch glucosyltransferase, isoform 1 [mips] Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase [SGD] GSY1, Chr VI from 174256-176382, reverse complement [yeast_orfs2] Chr VI from 174256-176382, reverse complement [yeastorf3.fasta] Chr VI from 176383-174257, reverse complement [yeastorf5.fasta] GSY1 SGDID:S0001911, Chr VI from 176383-174257, reverse complement, Verified ORF [yeastflysequest.fasta] Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, enviromental stress, and entry into stationary phase [Saccharomyces cerevisiae]gi\|136753\|sp\|P23337\|GYS1_YE [nrdb_11152004_Chlamydomonas] GSY1 SGDID:S000001911, Chr VI from 176383-174257, reverse complement, Verified ORF, "Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] glycogen synthase isoform 1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; Gsy1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Glycogen [starch] synthase isoform 1 [nr-271106-contam.fasta] glycogen synthase [nr-271106-contam.fasta] GSY1 SGDID:S000001911, Chr VI from 176383-174257, reverse complement, Verified ORF, "Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Glycogen [starch] synthase isoform 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GSY1 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GSY1 SGDID:S000001911, Chr VI from 176391-174265, Genome Release 64-1-1, reverse complement, Verified ORF, "Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase" [yeast-030211-contam.fasta] GSY1 SGDID:S000001911, Chr VI from 176391-174265, Genome Release 64-2-1, reverse complement, Verified ORF, "Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress" [20141208_orf_trans.fasta] YFR015C SGDID:S000001911, Chr VI from 176391-174265, Genome Release 64-1-1, reverse complement, Verified ORF, "Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	708 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] mitochondrion [IDA ]
Biological Process:	glycogen biosynthetic process [IGI][IMP][ISS]
Molecular Function:	glycogen (starch) synthase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MARDLQNHLL FEVATEVTNR VGGIYSVLKS KAPVTVAQYG DNYTLLGPLN KATYESEVEK  60
   61 LDWEDESIFP EELLPIQKTL MSMREKGVNF VYGNWLIEGA PRVILFELDS VRHFLNEWKA 120
  121 DLWSLVGIPS PEHDHETNDA ILLGYVVVWF LGEVSKLDSS HAIIGHFHEW LAGVALPLCR 180
  181 KKRIDVVTIF TTHATLLGRY LCAAGDVDFY NNLQYFDVDQ EAGKRGIYHR YCIERAAAHT 240
  241 ADVFTTVSQI TALEAEHLLK RKPDGILPNG LNVVKFQAVH EFQNLHALKK DKINDFVRGH 300
  301 FHGCFDFDLD NTVYFFIAGR YEYKNKGADM FIESLARLNY RLKVSGSKKT VVAFLIMPAK 360
  361 TNSFTVEALK SQAIVKSLEN TVNEVTASIG KRIFEHTMRY PHNGLESELP TNLDELLKSS 420
  421 EKVLLKKRVL ALRRPYGELP PVVTHNMCDD ANDPILNQIR HVRLFNDSSD RVKVIFHPEF 480
  481 LNANNPILGL DYDEFVRGCH LGVFPSYYEP WGYTPAECTV MGVPSITTNV SGFGAYMEDL 540
  541 IETDQAKDYG IYIVDRRFKS PDESVEQLAD YMEEFVNKTR RQRINQRNRT ERLSDLLDWK 600
  601 RMGLEYVKAR QLGLRRAYPE QFKQLVGETI SDANMNTLAG GKKFKIARPL SVPGSPKVRS 660
  661 NSTVYMTPGD LGTLQDANNA DDYFNLSTNG AIDNDDDDND TSAYYEDN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		GSY1 GSY2
View Details	Krogan NJ, et al. (2006)		ADE5,7 DAP1 GLG1 GLG2 GSY1 GSY2
View Details	Ho Y, et al. (2002)		ADH4 CDC28 CLU1 COP1 FIN1 FPR3 FPR4 GFA1 GLC7 GLC8 GSY1 GSY2 MDH1 MET10 NOP7 NUG1 PDC5 REG1 RFC4 RPN1 SDS22 SMC3 TCP1 UBR1 URA7 YGL081W YPI1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	MIH1	experimental1 - mih1-1	McCusker D, et al (2007)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	KAP104	GSY1	1	Unpublished Fields Lab Data
View Screen	RAD1	GSY1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GSY1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..68]	deduced	N/A	No confident structure predictions are available.
2	View Details	[69..359]	MSA	1.421879	View MSA. No confident structure predictions are available.
3	View Details	[360..656]	MSA	277.951997	View MSA. No confident structure predictions are available.
4	View Details	[657..708]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
glycogen phosphorylase activity	4.85572378579745	bayes_pls_golite062009
phosphorylase activity	3.90117606315103	bayes_pls_golite062009
transferase activity, transferring hexosyl groups	3.13569872928774	bayes_pls_golite062009
catalytic activity	2.42803950895063	bayes_pls_golite062009
transferase activity, transferring glycosyl groups	2.09333759861004	bayes_pls_golite062009
glucosyltransferase activity	1.90464350723601	bayes_pls_golite062009
transferase activity	1.88974698466478	bayes_pls_golite062009
UDP-glycosyltransferase activity	1.65897712372683	bayes_pls_golite062009
UDP-glucosyltransferase activity	1.65637638312265	bayes_pls_golite062009
UDP-N-acetylglucosamine 2-epimerase activity	0.988654552791302	bayes_pls_golite062009
oxidoreductase activity	0.983614980754643	bayes_pls_golite062009
carbohydrate phosphatase activity	0.459821108931138	bayes_pls_golite062009
binding	0.45826376378159	bayes_pls_golite062009
alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity	0.290421196423307	bayes_pls_golite062009
protein binding	0.228850008528478	bayes_pls_golite062009
sugar binding	0.0223079311271377	bayes_pls_golite062009

Term	Confidence	Notes
glycogen phosphorylase activity	5.43556332287206	bayes_pls_golite062009
phosphorylase activity	4.4363209226962	bayes_pls_golite062009
transferase activity, transferring hexosyl groups	3.70184884300914	bayes_pls_golite062009
catalytic activity	2.65148466065976	bayes_pls_golite062009
transferase activity, transferring glycosyl groups	2.62403914335522	bayes_pls_golite062009
glucosyltransferase activity	2.48423599937201	bayes_pls_golite062009
UDP-glucosyltransferase activity	2.22592863643386	bayes_pls_golite062009
transferase activity	2.18054407163329	bayes_pls_golite062009
UDP-glycosyltransferase activity	2.13085705769565	bayes_pls_golite062009
UDP-N-acetylglucosamine 2-epimerase activity	1.55280519670033	bayes_pls_golite062009
oxidoreductase activity	0.974556371594238	bayes_pls_golite062009
alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity	0.849686864309466	bayes_pls_golite062009
carbohydrate phosphatase activity	0.78952375993773	bayes_pls_golite062009
binding	0.399722153246393	bayes_pls_golite062009
mannosyltransferase activity	0.253888301381021	bayes_pls_golite062009
protein binding	0.198267161626932	bayes_pls_golite062009
sugar binding	0.14686473522482	bayes_pls_golite062009
drug binding	0.102804615007901	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2