Protein Overview: BOI2

General Information:
Name(s) found:	BOI2 / YER114C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|728958\|sp\|P39969.1\|BOI2_YEAST RecName: Full=Protein BOI2; AltName: Full=Protein BEB1 [NCBI NR] gi\|6320960\|ref\|NP_011039.1\| Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; Boi2p [Saccharomyces cerevisiae] [NCBI NR] gi\|603353\|gb\|AAC03212.1\| Boi2p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S50617 BEB1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] Protein BOI2 OS=Saccharomyces cerevisiae GN=BOI2 PE=1 SV=1 [Swiss-Prot] BOI2 Budding protein [mips] Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain [SGD] BOI2, Chr V from 390586-393708, reverse complement [yeast_orfs2] Chr V from 390586-393708, reverse complement [yeastorf3.fasta] Chr V from 393708-390586, reverse complement [yeastorf5.fasta] (P39969) BOI2 protein (BEB1 protein) [4932.FASTACCERV.txt] BOI2 SGDID:S0000916, Chr V from 393708-390586, reverse complement, Verified ORF [yeastflysequest.fasta] Boi2p [Saccharomyces cerevisiae]gi\|603353\|gb\|AAC03212.1\| Boi2p [Saccharomyces cerevisiae]gi\|728958\|sp\|P39969\|BOI2_YEAST BOI2 protein (BEB1 protein)gi\|1077033\|pir\|\|S50617 BEB1 protein - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] BOI2 SGDID:S000000916, Chr V from 393708-390586, reverse complement, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (plecks [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein BOI2 (Protein BEB1) [nr-271106-contam.fasta] Boi2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; Boi2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] BOI2 SGDID:S000000916, Chr V from 393708-390586, reverse complement, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Protein BOI2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BOI2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] BOI2 SGDID:S000000916, Chr V from 393712-390590, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" [yeast-030211-contam.fasta] BOI2 SGDID:S000000916, Chr V from 393712-390590, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YER114C SGDID:S000000916, Chr V from 393712-390590, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1040 amino acids
Gene Ontology:
Cellular Component:	cellular bud [IDA] site of polarized growth [IDA] cellular bud neck [IDA]
Biological Process:	Rho protein signal transduction [IGI][IPI]
Molecular Function:	phospholipid binding [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSNDREVPTL SQLNTTVSRD KDVSDTLSPD FDSKGSATGR DGGNFPMYIA INEYFKRMED  60
   61 ELDMKPGDKI KVITDDEEYK DGWYFGRNLR TNEEGLYPVV FTQKITVEKA PTLMRAKSTK 120
  121 RIYSPLTNED PLLSSTFISE NDSNSELPTP QPIETAASIS RTANGKIERN LSLKNTMSDI 180
  181 DNALLEFKDD SIGPPDRFIN SGRDEEHSIT HETILSATDG LDVVESNSKP TTSSSTGFLN 240
  241 GDLENQATLI NGIDTTKLNP VEAEFWSPEE ITAYFIMEGY DVQSASRFQK HKISGKILLE 300
  301 LELVHLKELD INSFGTRFEI FKEIEKIKEA IRTNGRSLNR ASKTNNANIY NQLMPPANVD 360
  361 QRASYRGHVR KTSQSLEDLP SQQNFIPTPR NTRNSSASKH RPKSLVFDSQ EANANIAPDV 420
  421 QIPQVVEEMA GNENLFVSPR RAPKPPSYPS PAQPPKSPLL NNTRTSPSPA QLYSWQSPTL 480
  481 SFSGPKRTSY IDQYSSSDSN FNSRSALPKN NQGGGKALSP IPSPTRNSVR NEDSEGKLTS 540
  541 SSKRNSVPYY GYAPESSSDR KSSCSSHEEE QFQETMNTFE RPTSSIYADG STIASISNDK 600
  601 LAHEKEGKKK PTRHSSSLSS KSKSDSRRNS SLKRSSSASR TSSFKKSSFM LSPFRQQFTD 660
  661 NAARSSSPEE NPITSMPSEK NSSPIVDKKS SKKSRSKRRS VSAKEAEIFT ETVKDDKNKR 720
  721 SASEAIKGET LKGKSLRQMT ARPVAKKKQT SAFIEGLRSI SVKEAMKDAD FSGWMSKKGS 780
  781 GAMSTWKTRF FTLHGTRLSY FSSTTDTRER GLIDITAHRV VPAKEDDKLV SLYAASTGKG 840
  841 RYCFKLLPPQ PGSKKGLTFT QPRTHYFAVD NKEEMRGWMA ALIKTTIDID TSVPIISSYT 900
  901 TPTVSLSKAQ EMLAEAREET KLREQQMLEN EEDEDQFLWD QQQLQQQQHD NNQGQADRTI 960
  961 SASTQRTSDE DNTISTPNLS SANNTTIGSN GFSSPFLLAS GLLSPGVARN SSMRGTEKKG1020
 1021 KFSTEEDYFG DNSKHKTDKI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BOI2 CDC24
View Details	Gavin AC, et al. (2006)		BOI2 CDC24
View Details	Gavin AC, et al. (2002)		BEM1 BOI2 CDC24 RSC2
View Details	Ho Y, et al. (2002)		ADR1 BMH1 BNR1 BOI2 CSR2 CYK3 GSY2 KCS1 NTH1 REG1 SOK1 STU1 SVL3 YFR017C YIL028W
View Details	Qiu et al. (2008)		BEM1 BNI1 BOI1 BOI2 BUD6 IQG1 OSH2 PEA2 PXL1 SPA2 ZDS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BOI2	BEM1	2	Drees BL, et al. (2001)
View Screen	BOI2	YEL043W	2	Unpublished Fields Lab Data
View Screen	BOI2	DSE1	2	Drees BL, et al. (2001)
View Screen	BOI2	YPS6	2	Unpublished Fields Lab Data
View Screen	BOI2	CLA4	2	Drees BL, et al. (2001)
View Screen	BOI2	MSB1	2	Drees BL, et al. (2001)
View Screen	BOI2	MRS6	2	Drees BL, et al. (2001)
View Screen	BOI2	BPH1	1	Unpublished Fields Lab Data
View Screen	BOI2	SGA1	1	Unpublished Fields Lab Data
View Screen	BOI2	YLR076C	1	Unpublished Fields Lab Data
View Screen	BOI2	WHI2	1	Unpublished Fields Lab Data
View Screen	LIA1	BOI2	1	Unpublished Fields Lab Data
View Screen	SPP382	BOI2	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BOI2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..108]	PSI-BLAST	79.522879	CHICKEN SRC TYROSINE KINASE
2	View Details	[109..208]	PSI-BLAST	79.522879	CHICKEN SRC TYROSINE KINASE
3	View Details	[209..292]	PSI-BLAST	79.522879	CHICKEN SRC TYROSINE KINASE
4	View Details	[293..381]	deduced	N/A	No confident structure predictions are available.
5	View Details	[382..675]	ab initio	14.79	No description for 1m2vB was found.
6	View Details	[676..765]	ab initio	9.79	No description for 1ei3A was found.
7	View Details	[766..963]	PSI-BLAST	63.29073	Grp1
8	View Details	[964..1040]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.76

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2