Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
AGC1 | Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutama... | 1 | 1 | 0.9% | 0.00251 | 104304 |
[ |
ALD5 | Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport c... | 1 | 1 | 6.5% | 0.00435 | 56621 |
[ |
CBF5 | Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (s... | 1 | 1 | 4.6% | 0.00468 | 54705 |
[ |
CCH1 | Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environme... | 1 | 1 | 2.8% | 0.00111 | 234597 |
[ |
CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... | 2 | 3 | 11.8% | 0.0123 | 61907 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 2 | 11 | 5.6% | 0.0498 | 54545 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 4 | 9 | 46.3% | 0.139 | 16135 |
[ |
CTK2 | Beta subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates both RNA pol II subunit Rp... | 1 | 1 | 5.6% | 0.007 | 37905 |
[ |
DED81 | Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attach... | 1 | 1 | 5.6% | 0.00408 | 62207 |
[ |
DSS1 | 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA h... | 1 | 1 | 2.8% | 0.00233 | 110822 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 1 | 3 | 3.3% | 0.00806 | 93289 |
[ |
ENO1 | Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... | 1 | 4 | 3.9% | 0.0207 | 46816 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 1 | 4 | 3.9% | 0.0207 | 46914 |
[ |
GIT1 | Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sou... | 1 | 1 | 2.5% | 0.00437 | 57322 |
[ |
GLC7 | Type 1 serine/threonine protein phosphatase catalytic subunit, involved in many processes (eg: glyco... | 1 | 1 | 17.9% | 0.00725 | 35907 |
[ |
HGH1 | Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green flu... | 1 | 1 | 6.9% | 0.00574 | 44952 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 1 | 1 | 3.0% | 0.00321 | 80900 |
[ |
HSP82 | Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p... | 1 | 1 | 3.0% | 0.00319 | 81406 |
[ |
IPI3 | Essential component of the Rix1 complex (Rix1p, Ipi1p, Ipi3p) that is required for processing of ITS... | 1 | 1 | 4.5% | 0.00408 | 61773 |
[ |
KTR3 | Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of ... | 1 | 1 | 5.7% | 0.0056 | 47482 |
[ |
LSB3 | Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldric... | 1 | 1 | 8.2% | 0.00502 | 48547 |
[ |
LYS12 | Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in th... | 1 | 1 | 6.5% | 0.0061 | 40069 |
[ |
MAP1 | Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nasce... | 1 | 1 | 6.7% | 0.00585 | 43373 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 4 | 5 | 2.5% | 0.00674 | 195140 |
[ |
MNN1 | Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for add... | 1 | 1 | 3.8% | 0.00297 | 88529 |
[ |
MRPL8 | Mitochondrial ribosomal protein of the large subunit | 1 | 1 | 8.8% | 0.00951 | 26945 |
[ |
MUB1 | Protein that interacts with E2 and E3 enzymes Ubr2p and Rad6p and is required for ubiquitylation and... | 1 | 1 | 8.5% | 0.00365 | 72251 |
[ |
MUC1 | GPI-anchored cell surface glycoprotein (flocculin) required for pseudohyphal formation, invasive gro... | 1 | 1 | 2.8% | 0.00166 | 136110 |
[ |
NOP58 | Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... | 1 | 1 | 6.5% | 0.00443 | 56956 |
[ |
NSR1 | Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... | 1 | 1 | 7.7% | 0.00546 | 44535 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 1 | 1 | 2.2% | 0.00266 | 94104 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 1 | 1 | 5.2% | 0.00402 | 61495 |
[ |
PRP9 | Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the forma... | 1 | 1 | 6.4% | 0.00427 | 63030 |
[ |
RAD54 | DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; i... | 1 | 1 | 5.1% | 0.00252 | 101754 |
[ |
RGA2 | GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of se... | 1 | 1 | 2.8% | 0.00224 | 113291 |
[ |
RPL12B, RPL12A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... | 1 | 1 | 9.1% | 0.0137 | 17823 |
[ |
RPL17A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similari... | 1 | 2 | 13.0% | 0.0246 | 20549 |
[ |
RPL17B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similari... | 1 | 2 | 13.0% | 0.0246 | 20551 |
[ |
RPL2A, RPL2B | Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E.... | 3 | 7 | 16.5% | 0.0624 | 27408 |
[ |
RPL5 | Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... | 3 | 7 | 10.8% | 0.0533 | 33715 |
[ |
RPO21 | RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapep... | 1 | 1 | 2.3% | 0.00131 | 191610 |
[ |
RPS10A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Bp and has similari... | 1 | 2 | 20.0% | 0.0431 | 12739 |
[ |
RPS10B | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Ap and has similari... | 1 | 2 | 20.0% | 0.0431 | 12738 |
[ |
RPS14A | Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processin... | 2 | 3 | 16.8% | 0.0495 | 14537 |
[ |
RPS14B | Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processin... | 2 | 3 | 16.7% | 0.0492 | 14650 |
[ |
RPS25A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Bp and has similari... | 1 | 1 | 9.3% | 0.0209 | 12039 |
[ |
RPS25B | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Ap and has similari... | 1 | 1 | 9.3% | 0.0209 | 12009 |
[ |
RPS8B, RPS8A | Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to ra... | 1 | 1 | 10.5% | 0.0113 | 22490 |
[ |
Reverse_YCL024W | KCC4 SGDID:S000000529, Chr III from 79161-82274, Verified ORF, "Protein kinase of the bud neck invol... | 1 | 1 | 1.9% | 0.00218 | 116475 |
[ |
Reverse_YDR143C | SAN1 SGDID:S000002550, Chr IV from 743869-742037, reverse complement, Verified ORF, "Ubiquitin-prote... | 1 | 1 | 3.3% | 0.0037 | 65618 |
[ |
Reverse_YDR517W | GRH1 SGDID:S000002925, Chr IV from 1477229-1478347, Uncharacterized ORF, "Golgi localized protein co... | 1 | 1 | 3.5% | 0.00607 | 41119 |
[ |
Reverse_YER021W | RPN3 SGDID:S000000823, Chr V from 196947-198518, Verified ORF, "Essential, non-ATPase regulatory sub... | 1 | 1 | 7.6% | 0.00432 | 60393 |
[ |
Reverse_YER110C | KAP123 SGDID:S000000912, Chr V from 382099-378758, reverse complement, Verified ORF, "Karyopherin be... | 1 | 1 | 2.8% | 0.00203 | 122601 |
[ |
Reverse_YGR081C | SLX9 SGDID:S000003313, Chr VII from 643784-643152, reverse complement, Verified ORF, "Protein of unk... | 1 | 1 | 14.8% | 0.0107 | 24069 |
[ |
Reverse_YIL030C | SSM4 SGDID:S000001292, Chr IX from 300008-296049, reverse complement, Verified ORF, "Ubiquitin-prote... | 1 | 1 | 3.6% | 0.00171 | 151454 |
[ |
Reverse_YIR019C | MUC1 SGDID:S000001458, Chr IX from 393672-389569, reverse complement, Verified ORF, "GPI-anchored ce... | 1 | 1 | 2.1% | 0.00165 | 136110 |
[ |
Reverse_YLR003C | YLR003C SGDID:S000003993, Chr XII from 157729-156854, reverse complement, Uncharacterized ORF, "Hypo... | 1 | 1 | 9.3% | 0.00775 | 33394 |
[ |
Reverse_YNL078W | NIS1 SGDID:S000005022, Chr XIV from 479769-480992, Verified ORF, "Protein localized in the bud neck ... | 1 | 1 | 11.1% | 0.00555 | 45909 |
[ |
Reverse_YNL240C | NAR1 SGDID:S000005184, Chr XIV from 199978-198503, reverse complement, Verified ORF, "Nuclear archit... | 1 | 1 | 5.1% | 0.0046 | 54151 |
[ |
Reverse_YOL081W | IRA2 SGDID:S000005441, Chr XV from 171069-180308, Verified ORF, "GTPase-activating protein that nega... | 1 | 1 | 1.0% | 7.35E-4 | 351669 |
[ |
Reverse_YOR112W | YOR112W SGDID:S000005638, Chr XV from 531508-533793, Uncharacterized ORF, "Hypothetical protein" | 1 | 1 | 4.1% | 0.00297 | 84769 |
[ |
Reverse_YOR209C | NPT1 SGDID:S000005735, Chr XV from 737726-736437, reverse complement, Verified ORF, "Nicotinate phos... | 1 | 1 | 8.2% | 0.00526 | 49019 |
[ |
Reverse_YPL253C | VIK1 SGDID:S000006174, Chr XVI from 73006-71063, reverse complement, Verified ORF, "Protein that for... | 1 | 1 | 7.1% | 0.00349 | 75730 |
[ |
Reverse_YPR057W | BRR1 SGDID:S000006261, Chr XVI from 672468-673493, Verified ORF, "snRNP protein component of spliceo... | 1 | 1 | 12.0% | 0.00662 | 39860 |
[ |
Reverse_YPR088C | SRP54 SGDID:S000006292, Chr XVI from 713026-711401, reverse complement, Verified ORF, "Signal recogn... | 1 | 1 | 3.5% | 0.00417 | 59624 |
[ |
SEC10 | Essential 100kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p,... | 1 | 1 | 3.7% | 0.0026 | 100342 |
[ |
SEO1 | Putative permease, member of the allantoate transporter subfamily of the major facilitator superfami... | 1 | 1 | 5.2% | 0.00382 | 68842 |
[ |
SKI2 | Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic e... | 1 | 1 | 3.1% | 0.00176 | 146058 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 2 | 2 | 15.0% | 0.0179 | 29280 |
[ |
SPS100 | Protein required for spore wall maturation; expressed during sporulation; may be a component of the ... | 1 | 1 | 13.8% | 0.00694 | 34226 |
[ |
SUR1 | Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex... | 1 | 1 | 7.6% | 0.00592 | 44808 |
[ |
TDH2 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... | 3 | 4 | 16.3% | 0.0273 | 35847 |
[ |
TDH3 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... | 2 | 4 | 15.7% | 0.0273 | 35747 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 1 | 1 | 6.3% | 0.00494 | 50033 |
[ |
TIP20 | Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion ... | 1 | 1 | 7.1% | 0.00323 | 81167 |
[ |
TOM70 | Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial... | 1 | 1 | 8.6% | 0.00367 | 70123 |
[ |
TRK2 | Component of the Trk1p-Trk2p potassium transport system | 1 | 1 | 2.9% | 0.00254 | 101086 |
[ |
TRL1 | tRNA ligase, required for tRNA splicing; composed of three essential domains containing the phosphod... | 1 | 1 | 3.6% | 0.00274 | 95337 |
[ |
UTP20 | Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA | 1 | 1 | 1.0% | 9.08E-4 | 287559 |
[ |
WHI3 | RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci; cytoplasmic retention factor for ... | 1 | 1 | 4.5% | 0.00342 | 71253 |
[ |
YGR015C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... | 1 | 1 | 4.9% | 0.0069 | 37937 |
[ |
YKR017C | Putative protein of unknown function; contains a RING finger motif | 1 | 1 | 8.7% | 0.00411 | 63569 |
[ |
YPS7 | Putative GPI-anchored aspartic protease, located in the cytoplasm and endoplasmic reticulum | 1 | 1 | 3.2% | 0.0038 | 64498 |
[ |
ZRG8 | Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondr... | 1 | 1 | 4.3% | 0.0021 | 119350 |