DTASelect v2.0.16 /data/1/catclw/Projects/Jamie/2and3 /garibaldi/people-b/applications/yates/dbase/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta SEQUEST 3.0 in SQT format. -p 1 --fp 0.1 --modstat --sp --noxc --nodcn --extra -e con true Use criteria 0.0 Minimum peptide confidence 0.1 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling con Exclude protein names matching 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YBR109C 4 9 46.3% 147 16135 4.3 U CMD1 SGDID:S000000313, Chr II from 458356-457913, reverse complement, Verified ORF, "Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin" * Jamie_phos_02_itms_37.13390.13390.4 4.426 0.2932 100.0 4111.3364 4109.526 7643.0 6 4.962 18.1 1 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q * Jamie_phos_02_itms_37.13402.13402.3 5.3637 0.1528 100.0 4113.6846 4109.526 7248.9 1 6.365 25.7 4 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q * Jamie_phos_02_itms_36.12982.12982.3 4.4717 0.2759 100.0 3908.6042 3909.288 8187.7 1 5.357 21.3 2 L.GLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q * Jamie_phos_02_itms_34.12526.12526.3 6.17 0.519 100.0 3366.6243 3366.7217 8673.5 1 8.23 30.0 2 K.LTDAEVDDMLREVSDGSGEINIQQFAALLSK.- YMR230W 1 2 20.0% 105 12738 9.1 U RPS10B SGDID:S000004843, Chr XIII from 732413-732464,732875-733140, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Ap and has similarity to rat ribosomal protein S10" YOR293W 1 2 20.0% 105 12739 8.8 U RPS10A SGDID:S000005819, Chr XV from 867095-867146,867584-867849, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Bp and has similarity to rat ribosomal protein S10" Jamie_phos_02_itms_32.11764.11764.2 3.5246 0.4251 100.0 2739.892 2740.9849 10756.3 1 8.073 32.5 2 K.TQFSWQYYYYTLTEEGVEYLR.E YER133W 1 1 17.9% 312 35907 5.5 U GLC7 SGDID:S000000935, Chr V from 432491-432667,433193-433954, Verified ORF, "Catalytic subunit of type 1 serine/threonine protein phosphatase, involved in many processes including glycogen metabolism, sporulation, and mitosis; interacts with multiple regulatory subunits; predominantly isolated with Sds22p" * Jamie_phos_02_itms_48.17414.17414.4 2.9265 0.1703 93.0 6458.4966 6453.1533 8901.3 124 4.41 12.1 1 R.PTDIPDVGLLCDLLWSDPDKDIVGWSENDRGVSFTFGPDVVNRFLQKQDMELICRA.H YCR031C 2 3 16.8% 137 14537 10.7 U RPS14A SGDID:S000000627, Chr III from 178215-178209,177901-177495, reverse complement, Verified ORF, "Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Bp and similar to E. coli S11 and rat S14 ribosomal proteins" YJL191W 2 3 16.7% 138 14650 10.5 U RPS14B SGDID:S000003727, Chr X from 73786-73795,74204-74610, Verified ORF, "Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Ap and similar to E. coli S11 and rat S14 ribosomal proteins" Jamie_phos_02_itms_17.06120.06120.2 2.9834 0.318 100.0 2584.9922 2585.876 7399.6 5 5.436 31.8 2 R.IYASFNDTFVHVTDLSGKETIAR.V Jamie_phos_02_itms_17.06104.06104.3 3.1753 0.239 100.0 2585.7544 2585.876 5672.0 77 5.008 26.1 1 R.IYASFNDTFVHVTDLSGKETIAR.V YFR031C-A 3 7 16.5% 254 27408 11.1 U RPL2A SGDID:S000002104, Chr VI from 221406-221403,221255-220495, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E. coli L2 and rat L8 ribosomal proteins" YIL018W 3 7 16.5% 254 27408 11.1 U RPL2B SGDID:S000001280, Chr IX from 316766-316769,317170-317930, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures" Jamie_phos_02_itms_15.05566.05566.3 5.5868 0.4521 100.0 2493.7744 2493.8247 9330.2 1 7.59 38.1 3 R.LREEIFIANEGVHTGQFIYAGK.K Jamie_phos_02_itms_14.05212.05212.3 4.5231 0.3123 100.0 2622.5344 2621.9988 10737.4 1 6.488 35.2 2 R.LREEIFIANEGVHTGQFIYAGKK.A Jamie_phos_02_itms_13.04697.04697.3 3.7918 0.2884 100.0 2098.8542 2099.3108 5964.1 2 5.411 37.5 2 R.ASGNYVIIIGHNPDENKTR.V YJR009C 3 4 16.3% 332 35847 7.0 U TDH2 SGDID:S000003769, Chr X from 454595-453597, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " * Jamie_phos_02_itms_30.10837.10837.3 5.6915 0.297 100.0 3304.8245 3304.727 10836.1 1 7.49 31.0 1 K.IATFQERDPANLPWASLNIDIAIDSTGVFK.E * Jamie_phos_02_itms_31.11218.11218.3 4.54 0.2307 100.0 4094.5444 4090.579 9871.4 19 4.961 19.4 1 K.IATFQERDPANLPWASLNIDIAIDSTGVFKELDTAQK.H Jamie_phos_02_itms_17.06078.06078.2 4.8002 0.3681 100.0 2128.2322 2128.3484 8637.3 1 7.697 68.8 2 K.FVKLVSWYDNEYGYSTR.V YGR192C 2 4 15.7% 332 35747 7.0 U TDH3 SGDID:S000003424, Chr VII from 883815-882817, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " * Jamie_phos_02_itms_26.09313.09313.3 4.3248 0.2501 92.6 3571.4644 3571.8762 8807.9 1 4.441 22.1 2 K.GVLGYTEDAVVSSDFLGDSHSSIFDASAGIQLSPK.F Jamie_phos_02_itms_17.06078.06078.2 4.8002 0.3681 100.0 2128.2322 2128.3484 8637.3 1 7.697 68.8 2 K.FVKLVSWYDNEYGYSTR.V YPL124W 2 2 15.0% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * Jamie_phos_02_itms_14.05320.05320.3 3.8651 0.2672 100.0 2581.2544 2581.8882 8466.6 6 4.915 31.2 1 T.DKFASQNVIDDQRLEIKYLER.I * Jamie_phos_02_itms_16.05946.05946.2 3.5209 0.3366 90.0 2036.3121 2037.1449 6093.1 1 6.208 50.0 1 R.SSQIHIENES*TEDILKI.L Reverse_YGR081C 1 1 14.8% 210 24069 10.0 U SLX9 SGDID:S000003313, Chr VII from 643784-643152, reverse complement, Verified ORF, "Protein of unknown function; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant" * Jamie_phos_02_itms_42.15320.15320.3 2.8082 0.1175 92.0 3492.5044 3488.7515 9513.0 9 4.404 21.7 1 E.KPQHLFARPDSEIDYSEDLIGNNAVDPGFNQ.S YHR139C 1 1 13.8% 326 34226 5.4 U SPS100 SGDID:S000001181, Chr VIII from 379202-378222, reverse complement, Verified ORF, "Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall" * Jamie_phos_02_itms_89.32214.32214.3 1.845 0.1815 100.0 4478.6646 4475.7393 8616.1 4 4.89 13.6 1 V.VVSSGTSHNSTVASWTSVDFPVVYASDGSSGHDGSGIGFISNTSI.P YJL177W 1 2 13.0% 184 20551 10.9 U RPL17B SGDID:S000003713, Chr X from 90784-91092,91410-91655, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similarity to E. coli L22 and rat L17 ribosomal proteins" YKL180W 1 2 13.0% 184 20549 10.9 U RPL17A SGDID:S000001663, Chr XI from 109274-109582,109889-110134, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similarity to E. coli L22 and rat L17 ribosomal proteins; copurifies with the components of the outer kinetochore DASH complex" Jamie_phos_02_itms_13.04777.04777.3 4.4404 0.4599 100.0 2701.4343 2702.0337 9106.0 1 7.512 33.7 2 R.INKYESSPSHIELVVTEKEEAVAK.A Reverse_YPR057W 1 1 12.0% 341 39860 6.6 U BRR1 SGDID:S000006261, Chr XVI from 672468-673493, Verified ORF, "snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed" * Jamie_phos_02_itms_15.05368.05368.3 1.3316 0.2334 96.5 4777.5244 4779.435 4067.6 13 4.597 8.1 1 S.SKHRTRKQLNMHNKISIANTRLAEQRVSKLYEIVDPNVSSD.S YFR028C 2 3 11.8% 551 61907 8.0 U CDC14 SGDID:S000001924, Chr VI from 210056-208401, reverse complement, Verified ORF, "Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit" * Jamie_phos_02_itms_38.13697.13697.2 2.7374 0.3175 100.0 2521.7122 2519.7295 10529.6 1 5.204 33.3 1 R.DAGYSNADFEITIQDVVYGVWR.A * Jamie_phos_02_itms_30.10954.10954.4 5.8618 0.2558 100.0 4997.5767 4998.4727 10812.4 1 5.737 20.2 2 K.GLIDLHSFNLESYEKYEHVEFGDFNVLTPDFIAFASPQEDHPK.G Reverse_YNL078W 1 1 11.1% 407 45909 9.9 U NIS1 SGDID:S000005022, Chr XIV from 479769-480992, Verified ORF, "Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network" * Jamie_phos_02_itms_5.02003.02003.4 3.6117 0.1544 93.5 5066.1367 5070.871 7314.6 98 4.485 15.2 1 M.TTPLSGPQSKPLKRSISRNRLYPPRSSTAYMQSRLFPIPTVAPDR.S YPL131W 3 7 10.8% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Jamie_phos_02_itms_22.08176.08176.3 6.3388 0.4091 100.0 3499.8542 3500.7944 6384.8 1 7.335 35.0 3 K.LGLDETYKGVEEVEGEYELTEAVEDGPRPFK.V * Jamie_phos_02_itms_22.08135.08135.4 4.5164 0.1277 97.6 3501.3765 3500.7944 6274.0 6 4.625 23.9 2 K.LGLDETYKGVEEVEGEYELTEAVEDGPRPFK.V * Jamie_phos_02_itms_22.08074.08074.3 4.7342 0.4292 100.0 3488.4844 3486.7676 7151.8 1 6.547 25.0 2 L.GLDETYKGVEEVEGEYELTEAVEDGPRPFKV.F YBL072C 1 1 10.5% 200 22490 10.7 U RPS8A SGDID:S000000168, Chr II from 89123-88521, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein" YER102W 1 1 10.5% 200 22490 10.7 U RPS8B SGDID:S000000904, Chr V from 363096-363698, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein" Jamie_phos_02_itms_14.05052.05052.3 3.6919 0.3052 100.0 2352.1443 2352.6555 8003.4 1 5.767 36.2 1 R.ALRIETGNFSWASEGISKKTR.I Reverse_YLR003C 1 1 9.3% 291 33394 8.2 U YLR003C SGDID:S000003993, Chr XII from 157729-156854, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_phos_03_itms_14.06129.06129.2 1.0177 0.2077 90.2 3072.7522 3070.5896 3413.5 33 4.678 11.5 1 F.LKVCNKGGNLSRYVDAVRINSMSFVIK.K YGR027C 1 1 9.3% 108 12039 10.3 U RPS25A SGDID:S000003259, Chr VII from 534462-534136, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Bp and has similarity to rat S25 ribosomal protein" YLR333C 1 1 9.3% 108 12009 10.3 U RPS25B SGDID:S000004325, Chr XII from 795899-795573, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Ap and has similarity to rat S25 ribosomal protein" Jamie_phos_02_itms_15.05371.05371.2 1.667 0.2725 100.0 1136.2922 1137.3213 8613.6 31 5.468 66.7 1 R.YVSVSVLVDR.L YDR418W 1 1 9.1% 165 17823 9.4 U RPL12B SGDID:S000002826, Chr IV from 1301606-1302103, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins" YEL054C 1 1 9.1% 165 17823 9.4 U RPL12A SGDID:S000000780, Chr V from 53218-52721, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Bp; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins" Jamie_phos_02_itms_18.06600.06600.2 3.2829 0.1886 97.5 1710.7722 1708.912 5628.3 1 4.966 57.1 1 K.HSGNIQLDEIIEIAR.Q YJL063C 1 1 8.8% 238 26945 9.8 U MRPL8 SGDID:S000003599, Chr X from 316391-315675, reverse complement, Verified ORF, "Mitochondrial ribosomal protein of the large subunit" * Jamie_phos_03_itms_30.11947.11947.3 2.2181 0.229 96.7 2579.8442 2576.0813 8876.2 118 4.533 27.5 1 D.NRKLMKRLFSEIAPRYLERPG.G YKR017C 1 1 8.7% 551 63569 6.1 U YKR017C SGDID:S000001725, Chr XI from 472992-471337, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; contains a RING finger motif" * Jamie_phos_03_itms_38.14913.14913.4 3.3971 0.1829 100.0 5833.7363 5837.4937 5708.3 230 5.195 13.5 1 I.LLQHYDWNEERLLEVWTEKMDELLVELGLSTTANIKKDNDYNSHFREV.E YNL121C 1 1 8.6% 617 70123 5.4 U TOM70 SGDID:S000005065, Chr XIV from 400539-398686, reverse complement, Verified ORF, "Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins" * Jamie_phos_02_itms_30.10876.10876.4 3.6888 0.0716 96.6 5852.7764 5851.76 8875.4 147 4.54 13.8 1 S.NLSACYVSVGDLKKVVEMSTKALELKPDYSKVLLRRASANEGLGKFADAMFDL.S YMR100W 1 1 8.5% 620 72251 6.9 U MUB1 SGDID:S000004706, Chr XIII from 466299-468161, Verified ORF, "Protein of unknown function, deletion causes multi-budding phenotype; has similarity to Aspergillus nidulans samB gene" * Jamie_phos_03_itms_51.19538.19538.4 1.8489 0.2082 94.6 6239.3765 6237.3926 4917.2 132 4.487 8.7 1 F.LGRIVPKQDDVIWSLQLLAFVSKYTYMKSTLQNVELVESLSFRSMAYKIKQRI.S Reverse_YOR209C 1 1 8.2% 429 49019 6.9 U NPT1 SGDID:S000005735, Chr XV from 737726-736437, reverse complement, Verified ORF, "Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus" * Jamie_phos_03_itms_33.13095.13095.3 2.9044 0.2068 92.5 4210.6445 4206.841 10699.2 45 4.469 19.9 1 T.IAKEVNLSDSYCIIKSFKPLKLVDHYHHSIKKTYE.V YFR024C-A 1 1 8.2% 451 48547 6.1 U LSB3 SGDID:S000002968, Chr VI from 203421-203375,203256-201948, reverse complement, Verified ORF, "Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization" * Jamie_phos_03_itms_29.11722.11722.3 2.6029 0.2538 100.0 3935.9644 3937.4937 10766.3 6 4.86 18.1 1 G.GVAAVFAYSKSKGLFAGVSVEGSAIIERREANRKFYG.D YGR159C 1 1 7.7% 414 44535 4.9 U NSR1 SGDID:S000003391, Chr VII from 807661-806417, reverse complement, Verified ORF, "Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis" * Jamie_phos_02_itms_35.12583.12583.3 4.0896 0.328 100.0 3536.8442 3536.8762 4189.4 1 6.016 29.8 1 K.FGDTPSEPSDTLFLGNLSFNADRDAIFELFAK.H Reverse_YER021W 1 1 7.6% 523 60393 5.6 U RPN3 SGDID:S000000823, Chr V from 196947-198518, Verified ORF, "Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control" * Jamie_phos_02_itms_71.25715.25715.4 3.088 0.2214 98.7 4508.8965 4507.1055 10999.7 35 4.739 17.1 1 T.KKETNVSYFSAPYSNRLEASGPVSSKHNSTIFKLLNKKFS.S YPL057C 1 1 7.6% 382 44808 9.0 U SUR1 SGDID:S000005978, Chr XVI from 453054-451906, reverse complement, Verified ORF, "Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p" * Jamie_phos_02_itms_69.25091.25091.2 1.6662 0.2708 96.5 3228.5322 3226.81 7513.9 202 4.828 17.9 1 T.SPLGVSNDVMGSVPRHPFFLKALKSLKHY.D YGL145W 1 1 7.1% 701 81167 6.3 U TIP20 SGDID:S000003113, Chr VII from 230248-232353, Verified ORF, "Peripheral membrane protein required for fusion of COPI vesicles with the ER, prohibits back-fusion of COPII vesicles with the ER, may act as a sensor for vesicles at the ER membrane" * Jamie_phos_03_itms_18.07529.07529.4 1.9791 0.1617 97.6 5801.5366 5804.4507 5139.1 201 4.638 10.5 1 K.LLEAQWDTQKFASTSVGLVKCLRENSTKLYQLSLLYLPLEEETQNGDSER.P Reverse_YPL253C 1 1 7.1% 647 75730 7.8 U VIK1 SGDID:S000006174, Chr XVI from 73006-71063, reverse complement, Verified ORF, "Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p" * Jamie_phos_02_itms_72.26215.26215.3 1.4878 0.2702 91.2 5239.554 5237.019 5915.3 246 4.377 7.8 1 C.LQTQVEESITLLKNVNKSDNLNLLFFSKTDSILKKLVLAIPTECSE.K YGR187C 1 1 6.9% 394 44952 4.7 U HGH1 SGDID:S000003419, Chr VII from 871421-870237, reverse complement, Verified ORF, "Protein of unknown function with similarity to human HMG1 and HMG2; localizes to the cytoplasm" * Jamie_phos_03_itms_47.17911.17911.3 2.0126 0.2251 96.6 3018.0544 3020.6624 8365.8 18 4.562 20.2 1 D.IIKMIMDPEHGTRVIIQQGVTILVNLS.E YLR244C 1 1 6.7% 387 43373 7.9 U MAP1 SGDID:S000004234, Chr XII from 626333-625170, reverse complement, Verified ORF, "Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p" * Jamie_phos_03_itms_50.19107.19107.2 1.6961 0.2168 98.7 2860.9922 2863.246 6863.0 169 5.056 22.0 1 A.QFEHTLLVTEHGVEILTARNKKSPGG.P YOR310C 1 1 6.5% 511 56956 8.9 U NOP58 SGDID:S000005837, Chr XV from 898355-896820, reverse complement, Verified ORF, "Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA" * Jamie_phos_02_itms_44.15972.15972.3 4.03 0.26 100.0 3588.5942 3586.9495 11440.5 26 5.02 20.3 1 K.TAAEVSMGTEITQTDLDNINALAEQIVEFAAYR.E YER073W 1 1 6.5% 520 56621 8.3 U ALD5 SGDID:S000000875, Chr V from 304027-305589, Verified ORF, "Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed" * Jamie_phos_02_itms_47.17023.17023.3 3.3251 0.3182 100.0 3673.1343 3671.0405 10712.2 231 5.226 18.9 1 A.GTVWINTYNNFHQNVPFGGFGQSGIGREMGEAAL.S YIL094C 1 1 6.5% 371 40069 8.0 U LYS12 SGDID:S000001356, Chr IX from 187629-186514, reverse complement, Verified ORF, "Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate" * Jamie_phos_02_itms_65.23564.23564.2 1.9445 0.3324 100.0 2147.0723 2148.5076 8593.3 14 5.889 30.4 1 D.GAAALVGSLGVVPSANVGPEIVIG.E YDL030W 1 1 6.4% 530 63030 6.5 U PRP9 SGDID:S000002188, Chr IV from 397534-399126, Verified ORF, "Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex" * Jamie_phos_03_itms_24.09628.09628.4 2.1318 0.2104 92.1 3857.6565 3856.4487 9075.4 85 4.396 17.7 1 D.GQLQEEHLSGKSFDMPLGPDGLPMPYWLYKLHGL.D YBR118W 1 1 6.3% 458 50033 9.0 U TEF2 SGDID:S000000322, Chr II from 477665-479041, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" YPR080W 1 1 6.3% 458 50033 9.0 U TEF1 SGDID:S000006284, Chr XVI from 700592-701968, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" Jamie_phos_03_itms_35.13756.13756.3 3.6448 0.2942 100.0 2925.7444 2925.2898 10483.2 1 6.062 26.8 1 K.NMITGTSQADCAILIIAGGVGEFEAGISK.D YBR205W 1 1 5.7% 404 47482 8.7 U KTR3 SGDID:S000000409, Chr II from 633617-634831, Verified ORF, "Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family" * Jamie_phos_02_itms_31.11285.11285.2 2.1115 0.2144 91.2 2410.872 2411.738 6017.3 15 4.691 29.5 1 L.FFMSGSRSPEVPIAQGTSVSRVA.S YHR019C 1 1 5.6% 554 62207 5.8 U DED81 SGDID:S000001061, Chr VIII from 143551-141887, reverse complement, Verified ORF, "Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA" * Jamie_phos_02_itms_20.07337.07337.4 4.0555 0.1761 92.8 3579.2566 3579.7717 9078.6 13 4.423 21.1 1 K.DAITWLNEHDIKNEEGEDFKFGDDIAEAAER.K YAL038W 2 11 5.6% 500 54545 7.7 U CDC19 SGDID:S000000036, Chr I from 71787-73289, Verified ORF, "Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration" * Jamie_phos_02_itms_26.09466.09466.3 5.9758 0.2809 100.0 3083.1543 3080.5486 10376.9 1 5.801 33.3 2 I.SAGRIIYVDDGVLSFQVLEVVDDKTLKV.K * Jamie_phos_02_itms_29.10603.10603.2 6.3102 0.3966 100.0 2269.7922 2267.581 7781.9 1 7.374 68.4 9 R.IIYVDDGVLSFQVLEVVDDK.T YJL006C 1 1 5.6% 323 37905 8.1 U CTK2 SGDID:S000003543, Chr X from 424024-423053, reverse complement, Verified ORF, "Beta subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing; has similarity to cyclins" * Jamie_phos_02_itms_27.09857.09857.2 2.0713 0.2565 100.0 1926.8922 1927.1156 7842.5 6 5.061 38.2 1 R.YVLSLELINGESSINSST.R YAL067C 1 1 5.2% 593 68842 8.0 U SEO1 SGDID:S000000062, Chr I from 9017-7236, reverse complement, Verified ORF, "Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide" * Jamie_phos_03_itms_56.21364.21364.2 1.3031 0.195 91.3 3527.112 3529.0276 5326.2 24 4.688 18.3 1 R.LKWGFIPVKRQVEDLPDDLNSTEIVTISNSI.Q YLR044C 1 1 5.2% 563 61495 6.2 U PDC1 SGDID:S000004034, Chr XII from 234082-232391, reverse complement, Verified ORF, "Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism" * Jamie_phos_02_itms_44.15782.15782.3 3.5586 0.2086 100.0 3085.7944 3085.391 8916.8 1 5.616 28.6 1 K.EAVESADLILSVGALLSDFNTGSFSYSYK.T YGL163C 1 1 5.1% 898 101754 9.0 U RAD54 SGDID:S000003131, Chr VII from 196409-193713, reverse complement, Verified ORF, "DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family" * Jamie_phos_03_itms_51.19502.19502.3 1.7823 0.3324 92.9 5194.4346 5191.3115 4830.3 216 4.422 9.4 1 H.VIFVNLKPLQNELYNKLIKSREVKKVVKGVGGSQPLRAIGILKKLC.N Reverse_YNL240C 1 1 5.1% 491 54151 7.6 U NAR1 SGDID:S000005184, Chr XIV from 199978-198503, reverse complement, Verified ORF, "Nuclear architecture related protein; component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins; homologous to human Narf" * Jamie_phos_02_itms_30.11095.11095.3 2.8327 0.242 95.6 2786.9043 2785.9578 8840.1 1 4.515 29.2 1 S.STICGSCALCDSLTISVKELQDPET.S YGR015C 1 1 4.9% 328 37937 8.6 U YGR015C SGDID:S000003247, Chr VII from 522080-521094, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_phos_02_itms_16.05816.05816.2 2.2723 0.2381 100.0 1723.5322 1724.1119 9036.3 3 5.072 46.7 1 F.SMGGKIALLTTLYKNI.N YLR175W 1 1 4.6% 483 54705 8.9 U CBF5 SGDID:S000004165, Chr XII from 506136-507587, Verified ORF, "Component of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), probable rRNA pseudouridine synthase, binds to snoRNP Nop10p and also interacts with ribosomal biogenesis protein Nop53p" * Jamie_phos_02_itms_49.17864.17864.2 2.3525 0.173 97.5 2525.132 2523.947 9585.9 112 4.995 26.2 1 Y.GAKLMIPGLLRYEEGIELYDEI.V YNL197C 1 1 4.5% 661 71253 8.4 U WHI3 SGDID:S000005141, Chr XIV from 269594-267609, reverse complement, Verified ORF, "RNA binding protein that binds to and sequesters the G1 cyclin CLN3 mRNA; regulates cell fate and dose-dependently inhibits passage through Start by regulating the critical cell size requirement necessary for cell cycle progression" * Jamie_phos_03_itms_40.15391.15391.2 1.57 0.2471 97.6 3305.5322 3307.6594 9011.9 46 4.9 17.2 1 L.LRDNTEINEKIWGTSGIPSSINGYMSTPQP.S YNL182C 1 1 4.5% 555 61773 5.7 U IPI3 SGDID:S000005126, Chr XIV from 297629-295962, reverse complement, Uncharacterized ORF, "Protein required for cell viability; computational analysis of large-scale protein-protein interaction data suggests a possible role in assembly of the ribosomal large subunit" * Jamie_phos_03_itms_73.27394.27394.2 1.6945 0.288 97.5 2755.0522 2752.9946 9706.3 6 5.032 25.0 1 M.DEQVIFTTNTSGTIASVHSFEQINL.R YER033C 1 1 4.3% 1076 119350 4.9 U ZRG8 SGDID:S000000835, Chr V from 221286-218056, reverse complement, Verified ORF, "Cytoplasmic protein of unknown function, transcription is induced under conditions of zinc deficiency" * Jamie_phos_03_itms_28.11221.11221.3 1.7659 0.2294 98.7 5171.784 5175.3364 8217.1 4 4.738 12.2 1 N.NGDENESDNLYQKRLENGKETFNGNHGGHHDDASLGETVDNKEQFL.I Reverse_YOR112W 1 1 4.1% 761 84769 5.4 U YOR112W SGDID:S000005638, Chr XV from 531508-533793, Uncharacterized ORF, "Hypothetical protein" * Jamie_phos_03_itms_68.25683.25683.3 1.6692 0.1199 90.2 3306.5942 3310.8994 6033.2 85 4.377 17.5 1 I.PSKDLLGPAIVTLIKNAITNVDFLEISKELG.Y YGR254W 1 4 3.9% 437 46816 6.6 U ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose" YHR174W 1 4 3.9% 437 46914 6.0 U ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" Jamie_phos_02_itms_29.10546.10546.2 5.6941 0.4911 100.0 1822.6322 1823.0099 9206.4 1 9.042 68.8 4 R.SGETEDTFIADLVVGLR.T YER001W 1 1 3.8% 762 88529 7.2 U MNN1 SGDID:S000000803, Chr V from 153519-155807, Verified ORF, "Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family" * Jamie_phos_03_itms_53.20399.20399.2 1.1972 0.343 100.0 3441.9521 3441.6982 4963.1 68 5.063 12.5 1 E.EQRLHIDAVNTVWSHANKDNTRSFTEEEI.K YLR166C 1 1 3.7% 871 100342 6.3 U SEC10 SGDID:S000004156, Chr XII from 498046-495431, reverse complement, Verified ORF, "Essential 100kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis" * Jamie_phos_03_itms_30.12052.12052.4 2.988 0.118 92.9 3637.0964 3639.1406 6862.5 2 4.413 21.5 1 F.SEQVATQEVIHSENVIKLSKDLHTTLLTFDKL.D Reverse_YIL030C 1 1 3.6% 1319 151454 6.3 U SSM4 SGDID:S000001292, Chr IX from 300008-296049, reverse complement, Verified ORF, "Ubiquitin-protein ligase of the ER/nuclear envelope, required for degradation of Alpha2p and other proteins containing a Deg1 degradation signal; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation" * Jamie_phos_03_itms_41.15903.15903.4 2.5495 0.1429 97.5 5330.0166 5332.68 8265.5 265 4.65 14.1 1 P.ANNIDREPIDHEVEEVENEDVAHEDLTNQGETFHLIDKAIKSLCVSG.V YJL087C 1 1 3.6% 827 95337 7.8 U TRL1 SGDID:S000003623, Chr X from 272401-269918, reverse complement, Verified ORF, "tRNA ligase, required for tRNA splicing; composed of three essential domains containing the phosphodiesterase, polynucleotide kinase, and ligase activities required for ligation; localized at the inner membrane of the nuclear envelope" * Jamie_phos_02_itms_45.16363.16363.3 2.4424 0.2187 96.6 3450.9243 3450.7886 10108.7 39 4.544 20.7 1 Q.LAAMNINRSDFARMLYTHNVTAVAEYCDDS.F Reverse_YPR088C 1 1 3.5% 541 59624 8.6 U SRP54 SGDID:S000006292, Chr XVI from 713026-711401, reverse complement, Verified ORF, "Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain" * Jamie_phos_02_itms_21.07663.07663.2 2.7344 0.1036 94.3 2183.912 2182.6626 9188.4 156 4.797 33.3 1 Q.AINSLPGMKMITQMQKKFD.L Reverse_YDR517W 1 1 3.5% 372 41119 5.3 U GRH1 SGDID:S000002925, Chr IV from 1477229-1478347, Uncharacterized ORF, "Golgi localized protein component of the spindle assembly checkpoint; homolog of human GRASP65, which functions in postmitotic reassembly of Golgi stacks" * Jamie_phos_03_itms_86.31985.31985.3 2.1968 0.1719 94.5 1630.2244 1628.8834 8316.8 69 4.487 35.4 1 P.CHKVTPIRHLFGY.G YDR385W 1 3 3.3% 842 93289 6.3 U EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" YOR133W 1 3 3.3% 842 93289 6.3 U EFT1 SGDID:S000005659, Chr XV from 575098-577626, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" Jamie_phos_02_itms_30.11020.11020.3 4.0066 0.2855 100.0 2977.3743 2977.2573 7458.7 1 5.837 25.9 3 K.TDGNSFLINLIDSPGHVDFSSEVTAALR.V Reverse_YDR143C 1 1 3.3% 610 65618 5.2 U SAN1 SGDID:S000002550, Chr IV from 743869-742037, reverse complement, Verified ORF, "Ubiquitin-protein ligase, involved in the proteasome-dependent degradation of aberrant nuclear proteins; san1 mutations suppress sir4, spt16, and cdc68 mutations, suggesting a role in chromatin silencing" * Jamie_phos_02_itms_26.09608.09608.2 1.9633 0.2619 93.2 1814.4722 1811.865 6910.8 40 4.732 34.2 1 L.AGDPQHGGNGIGFSPGTGNA.I YDR349C 1 1 3.2% 596 64498 5.0 U YPS7 SGDID:S000002757, Chr IV from 1174168-1172378, reverse complement, Verified ORF, "Putative GPI-anchored aspartic protease, located in the cytoplasm and endoplasmic reticulum" * Jamie_phos_02_itms_48.17243.17243.2 1.8716 0.2338 100.0 2054.7722 2053.319 10126.7 80 5.086 30.6 1 K.FDLIPYVDPVSNAVSVGYP.I YLR398C 1 1 3.1% 1287 146058 6.7 U SKI2 SGDID:S000004390, Chr XII from 919019-915156, reverse complement, Verified ORF, "Putative RNA helicase, involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski3p and Ski8p; required for repressing propagation of dsRNA viruses" * Jamie_phos_03_itms_42.16191.16191.4 2.4709 0.2382 92.6 4207.177 4210.6104 7536.5 13 4.461 17.5 1 E.VDLKEVANANASNSLSITRSINHNQNSVRGSTAQLPFTPG.G YMR186W 1 1 3.0% 705 80900 4.8 U HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock" YPL240C 1 1 3.0% 709 81406 4.9 U HSP82 SGDID:S000006161, Chr XVI from 98625-96496, reverse complement, Verified ORF, "Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p" Jamie_phos_02_itms_17.06418.06418.3 4.2656 0.3768 100.0 2511.4443 2511.699 8343.4 1 5.799 38.8 1 K.TLVDITKDFELEETDEEKAER.E YKR050W 1 1 2.9% 889 101086 9.5 U TRK2 SGDID:S000001758, Chr XI from 527457-530126, Verified ORF, "Component of the Trk1p-Trk2p potassium transport system" * Jamie_phos_03_itms_59.22467.22467.2 1.3034 0.2094 97.6 2903.152 2902.2144 8604.0 57 4.902 20.0 1 T.MTLQQRELSGSSGNAARSRSFKDNLF.R YGR217W 1 1 2.8% 2039 234597 8.4 U CCH1 SGDID:S000003449, Chr VII from 924699-930818, Verified ORF, "Voltage-gated calcium channel involved in calcium influx in response to mating pheromones" * Jamie_phos_03_itms_58.21880.21880.4 1.6984 0.1402 95.6 7027.6963 7025.0024 7249.7 57 4.493 9.2 1 S.ESMEVKEEEKRPQQIKHYLKFVYPQKIQEYTHASLVARIRKKFFGGHRNEDTRDFKQ.F YIR019C 1 1 2.8% 1367 136110 4.3 U MUC1 SGDID:S000001458, Chr IX from 393672-389569, reverse complement, Verified ORF, "GPI-anchored cell surface glycoprotein required for diploid pseudohyphal formation and haploid invasive growth, transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p)" * Jamie_phos_03_itms_66.24981.24981.3 1.5982 0.1599 92.2 3662.3643 3658.7705 5703.3 18 4.389 14.2 1 S.TTESSSTPVTSSTTESSSAPVPTPSSSTTESSSAPVPT.P Reverse_YER110C 1 1 2.8% 1113 122601 4.6 U KAP123 SGDID:S000000912, Chr V from 382099-378758, reverse complement, Verified ORF, "Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1" * Jamie_phos_02_itms_57.20807.20807.2 1.8636 0.2737 97.4 3339.3323 3336.8125 8066.6 2 5.056 21.7 1 V.PRFNDFVKAFDGALAQSLSVLVELAVDQLTA.E YDR379W 1 1 2.8% 1009 113291 7.9 U RGA2 SGDID:S000002787, Chr IV from 1230157-1233186, Verified ORF, "GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth" * Jamie_phos_02_itms_48.17395.17395.2 2.0236 0.2165 97.6 3099.3323 3101.2212 9489.6 94 4.911 20.4 1 E.ETSSHARTVSIDDILNSTLEHDSNSIEE.Q YMR287C 1 1 2.8% 969 110822 9.0 U MSU1 SGDID:S000004900, Chr XIII from 845344-842435, reverse complement, Verified ORF, "RNase, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs" * Jamie_phos_03_itms_36.14270.14270.2 2.1038 0.2095 97.4 3176.3123 3178.5964 6328.2 9 5.043 19.2 1 V.FRCYKQLPLGEVAQQQYDSMITSTKKG.I YIL149C 4 5 2.5% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * Jamie_phos_02_itms_14.05101.05101.2 3.7342 0.4085 100.0 1548.6921 1549.721 7204.5 1 6.796 70.8 1 R.LVEFSNVNELQEK.N * Jamie_phos_02_itms_20.07163.07163.2 3.0335 0.1763 93.3 2117.632 2118.3916 9351.8 3 4.717 41.2 2 R.LVEFSNVNELQEKNVELL.N * Jamie_phos_02_itms_19.07052.07052.3 3.7466 0.2511 100.0 2662.5244 2661.9812 10217.4 1 6.025 34.5 1 R.LVEFSNVNELQEKNVELLNCIR.I * Jamie_phos_02_itms_15.05621.05621.2 4.6839 0.5009 100.0 2053.912 2054.1777 8999.0 1 8.204 50.0 1 K.AAINFSNVTDLTNNSTDGAK.I YCR098C 1 1 2.5% 518 57322 8.5 U GIT1 SGDID:S000000695, Chr III from 298601-297045, reverse complement, Verified ORF, "Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability" * Jamie_phos_03_itms_81.30131.30131.3 2.0415 0.2328 98.7 1384.8544 1380.5499 4749.2 32 4.73 33.3 1 T.GKIGSVVGVECFQ.P YDL140C 1 1 2.3% 1733 191610 5.6 U RPO21 SGDID:S000002299, Chr IV from 210562-205361, reverse complement, Verified ORF, "RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime" * Jamie_phos_03_itms_59.22322.22322.3 2.1094 0.197 97.6 4320.9844 4320.083 8686.3 6 4.622 16.4 1 V.PDWDGVIPTPAIIKPKPLWSGKQILSVAIPNGIHLQRFD.E YOR373W 1 1 2.2% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * Jamie_phos_02_itms_15.05422.05422.2 2.802 0.3447 100.0 2142.5122 2143.2659 6204.7 1 6.827 44.4 1 R.VEDITSISEVNTSFNETEK.Q Reverse_YIR019C 1 1 2.1% 1367 136110 4.3 U MUC1 SGDID:S000001458, Chr IX from 393672-389569, reverse complement, Verified ORF, "GPI-anchored cell surface glycoprotein required for diploid pseudohyphal formation and haploid invasive growth, transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p)" * Jamie_phos_03_itms_21.08626.08626.2 1.6342 0.292 92.2 3322.892 3320.856 6208.7 5 4.711 19.6 1 I.LYTNENYGYLQVTGKPGTVGIIKLSWLYK.L Reverse_YCL024W 1 1 1.9% 1037 116475 8.7 U KCC4 SGDID:S000000529, Chr III from 79161-82274, Verified ORF, "Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p" * Jamie_phos_03_itms_59.22286.22286.2 1.5446 0.2263 95.4 2356.2522 2358.801 10262.5 108 4.809 26.3 1 N.TEWVDYLSLVNPHSLLKMIV.I Reverse_YOL081W 1 1 1.0% 3079 351669 7.1 U IRA2 SGDID:S000005441, Chr XV from 171069-180308, Verified ORF, "GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, has similarity to Ira1p and human neurofibromin" * Jamie_phos_03_itms_24.09632.09632.2 1.605 0.2204 96.4 2920.0322 2920.122 10851.1 14 4.89 24.1 1 Q.EATYGTPTSNSPSAAAAAAAAASNLRQQLQ.T YBL004W 1 1 1.0% 2493 287559 7.0 U UTP20 SGDID:S000000100, Chr II from 227639-235120, Uncharacterized ORF, "Possible snoRNA-binding protein, based on computational analysis of large-scale protein-protein interaction data" * Jamie_phos_03_itms_43.16593.16593.3 2.0132 0.1477 90.5 2760.5344 2757.0442 8064.8 2 4.367 25.0 1 V.PELESISKLVADLNSYSSSRMHEY.D YPR021C 1 1 0.9% 902 104304 8.0 U AGC1 SGDID:S000006225, Chr XVI from 603354-600646, reverse complement, Verified ORF, "Mitochondrial transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism, ornithine synthesis, and the malate-aspartate NADH shuttle" * Jamie_phos_02_itms_11.04224.04224.2 1.1677 0.1936 100.0 923.15216 924.0166 3425.4 105 5.573 35.7 1 K.DKATMRSD.L Proteins Peptide IDs Spectra Unfiltered 11461 127925 160882 Filtered 78 94 128 Forward matches 61 77 111 Decoy matches 17 17 17 Forward FP rate 27.87 22.08 15.32 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0