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Protein Overview: RAD1

General Information:
Name(s) found: RAD1 / YPL022W [SGD]
Description(s) found:
Found 25 descriptions.
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  • Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1100 amino acids

    Gene Ontology:
    Cellular Component: nucleotide-excision repair factor 1 complex [IPILink to PubMed article][TASLink to PubMed article]
    nucleus [IDALink to PubMed article]
    Biological Process: nucleotide-excision repair, DNA incision, 5'-to lesion [IDALink to PubMed article][TASLink to PubMed article]
    double-strand break repair via single-strand annealing, removal of nonhomologous ends [IMPLink to PubMed articleLink to PubMed article]
    removal of nonhomologous ends [IMPLink to PubMed articleLink to PubMed article]
    meiotic mismatch repair [IMPLink to PubMed article]
    mitotic recombination [IMPLink to PubMed article]
    Molecular Function: DNA binding [IDALink to PubMed article]
    single-stranded DNA specific endodeoxyribonuclease activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSQLFYQGDS DDELQEELTR QTTQASQSSK IKNEDEPDDS NHLNEVENED SKVLDDDAVL  60
   61 YPLIPNEPDD IETSKPNIND IRPVDIQLTL PLPFQQKVVE NSLITEDALI IMGKGLGLLD 120
  121 IVANLLHVLA TPTSINGQLK RALVLVLNAK PIDNVRIKEA LEELSWFSNT GKDDDDTAVE 180
  181 SDDELFERPF NVVTADSLSI EKRRKLYISG GILSITSRIL IVDLLSGIVH PNRVTGMLVL 240
  241 NADSLRHNSN ESFILEIYRS KNTWGFIKAF SEAPETFVME FSPLRTKMKE LRLKNVLLWP 300
  301 RFRVEVSSCL NATNKTSHNK VIEVKVSLTN SMSQIQFGLM ECLKKCIAEL SRKNPELALD 360
  361 WWNMENVLDI NFIRSIDSVM VPNWHRISYE SKQLVKDIRF LRHLLKMLVT SDAVDFFGEI 420
  421 QLSLDANKPS VSRKYSESPW LLVDEAQLVI SYAKKRIFYK NEYTLEENPK WEQLIHILHD 480
  481 ISHERMTNHL QGPTLVACSD NLTCLELAKV LNASNKKRGV RQVLLNKLKW YRKQREETKK 540
  541 LVKEVQSQDT FPENATLNVS STFSKEQVTT KRRRTRGASQ VAAVEKLRNA GTNVDMEVVF 600
  601 EDHKLSEEIK KGSGDDLDDG QEENAANDSK IFEIQEQENE ILIDDGDAEF DNGELEYVGD 660
  661 LPQHITTHFN KDLWAEHCNE YEYVDRQDEI LISTFKSLND NCSLQEMMPS YIIMFEPDIS 720
  721 FIRQIEVYKA IVKDLQPKVY FMYYGESIEE QSHLTAIKRE KDAFTKLIRE NANLSHHFET 780
  781 NEDLSHYKNL AERKLKLSKL RKSNTRNAGG QQGFHNLTQD VVIVDTREFN ASLPGLLYRY 840
  841 GIRVIPCMLT VGDYVITPDI CLERKSISDL IGSLQNNRLA NQCKKMLKYY AYPTLLIEFD 900
  901 EGQSFSLEPF SERRNYKNKD ISTVHPISSK LSQDEIQLKL AKLVLRFPTL KIIWSSSPLQ 960
  961 TVNIILELKL GREQPDPSNA VILGTNKVRS DFNSTAKGLK DGDNESKFKR LLNVPGVSKI1020
 1021 DYFNLRKKIK SFNKLQKLSW NEINELINDE DLTDRIYYFL RTEKEEQEQE STDENLESPG1080
 1081 KTTDDNALHD HHNDVPEAPV

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) RAD1 RAD10 SAW1
    View Details (MIPS) Mewes HW, et al. (2004) RAD1 RAD10 RAD14
    View Details Krogan NJ, et al. (2006) DPS1 RAD1 RAD10 SAW1
    View Details Gavin AC, et al. (2002) ADH1 GIN4 HMO1 KCC4 PBP2 PDC1 PGI1 RAD1 RAD10 YEF3 YRA1
    View Details Ho Y, et al. (2002) ARC1 CAR2 CDC60 CPR1 CTF4 DEF1 DMA2 DUN1 FAA1 FAR1 FUM1 GDE1 GPD1 GPD2 HEF3 KGD1 LSP1 MDH1 MDM30 MSI1 PDC6 PIL1 PRO1 PRO2 PTC6 PYC1 RAD1 RAD10 RAD14 RAD16 RAD4 RNR2 SAH1 SAW1 SEC6 SEN1 SOD2 STE20 TFP1 TIF1, TIF2 TPS1 UBI4 UTP4 YHR033W
    View Details Qiu et al. (2008) ABF1 RAD1 RAD10 RAD14 RAD2 RAD7

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen RAD1 YDR026C 2 Unpublished Fields Lab Data
    View Screen RAD1 HRD3 2 Unpublished Fields Lab Data
    View Screen RAD1 GUF1 2 Unpublished Fields Lab Data
    View Screen RAD1 YLR227W-A, YML04... 2 Unpublished Fields Lab Data
    View Screen RAD1 TYW3 2 Unpublished Fields Lab Data
    View Screen RAD1 SFH1 2 Unpublished Fields Lab Data
    View Screen RAD1 YHR112C 2 Unpublished Fields Lab Data
    View Screen RAD1 ORC1 2 Unpublished Fields Lab Data
    View Screen RAD1 SWA2 2 Unpublished Fields Lab Data
    View Screen RAD1 SWR1 2 Unpublished Fields Lab Data
    View Screen RAD1 COY1 2 Unpublished Fields Lab Data
    View Screen RAD1 AVT5 2 Unpublished Fields Lab Data
    View Screen RAD1 YOR051C 2 Unpublished Fields Lab Data
    View Screen RAD1 RRT13 2 Unpublished Fields Lab Data
    View Screen RAD1 LCB4 2 Unpublished Fields Lab Data
    View Screen RAD1 AVT6 2 Unpublished Fields Lab Data
    View Screen RAD1 TPO1 2 Unpublished Fields Lab Data
    View Screen RAD1 CCA1 2 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RAD1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..299] PSI-BLAST 12.522879 Initiation factor 4a
    2 View Details [300..585] PSI-BLAST 3.0 RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
    3 View Details [586..742] PSI-BLAST 3.0 RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
    4 View Details [743..819] PSI-BLAST 28.0 Nucleotide excision repair enzyme UvrB
    5 View Details [820..989] PSI-BLAST 113.28043 Putative ATP-dependent RNA helicase Hef, nuclease domain
    6 View Details [990..1100] MSA 2.057993 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3 No functions predicted.
    4
    Term Confidence Notes
  • single-stranded DNA specific endodeoxyribonuclease activity
    		•  12.5797089932289 bayes_pls_golite062009
  • endodeoxyribonuclease activity
    		•  7.65460057398582 bayes_pls_golite062009
  • deoxyribonuclease activity
    		•  6.8949486660401 bayes_pls_golite062009
  • endonuclease activity
    		•  6.81743235298471 bayes_pls_golite062009
  • four-way junction helicase activity
    		•  4.48421726286311 bayes_pls_golite062009
  • DNA helicase activity
    		•  4.45575761476577 bayes_pls_golite062009
  • DNA strand annealing activity
    		•  3.51692202399009 bayes_pls_golite062009
  • excinuclease ABC activity
    		•  3.48615352002265 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  3.44605832131927 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  3.37084035853131 bayes_pls_golite062009
  • damaged DNA binding
    		•  2.98078816242626 bayes_pls_golite062009
  • DNA binding
    		•  2.97855002479178 bayes_pls_golite062009
  • helicase activity
    		•  2.83648239124034 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.79236389919005 bayes_pls_golite062009
  • nuclease activity
    		•  2.20113675721212 bayes_pls_golite062009
  • binding
    		•  2.17324322139334 bayes_pls_golite062009
  • hydrolase activity
    		•  2.07039693890326 bayes_pls_golite062009
  • hydrolase activity, acting on ester bonds
    		•  1.69645042781713 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  1.26255690081743 bayes_pls_golite062009
  • transposase activity
    		•  1.10140160488189 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  1.05548921907953 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  1.02983374615445 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides
    		•  1.01049916010827 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
    		•  1.00920723797259 bayes_pls_golite062009
  • catalytic activity
    		•  0.816447099398022 bayes_pls_golite062009
  • protein binding
    		•  0.71091195096997 bayes_pls_golite062009
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.84

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle