Protein Overview: HEM15

General Information:
Name(s) found:	HEM15 / YOR176W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|IBBYFC ferrochelatase (EC 4.99.1.1) precursor - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6324750\|ref\|NP_014819.1\| Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway; Hem15p [Saccharomyces cerevisiae] [NCBI NR] gi\|3769\|emb\|CAA38371.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|171660\|gb\|AAA34667.1\| ferrochelatase precursor (EC 4.99.1.1) [NCBI NR] gi\|1420427\|emb\|CAA99385.1\| HEM15 [Saccharomyces cerevisiae] [NCBI NR] gi\|123054\|sp\|P16622.1\|HEMH_YEAST RecName: Full=Ferrochelatase, mitochondrial; AltName: Full=Protoheme ferro-lyase; AltName: Full=Heme synthetase; Flags: Precursor [NCBI NR] (P16622) Ferrochelatase, mitochondrial precursor (EC 4.99.1.1) (Protoheme ferro-lyase) (Heme synthetase) [Swiss-Prot] Ferrochelatase, mitochondrial OS=Saccharomyces cerevisiae GN=HEM15 PE=1 SV=1 [Swiss-Prot] HEM15 Ferrochelatase precursor [mips] Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway [SGD] Chr XV from 662401-663582 [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 662400-663581 [yeastorf3.fasta] HEM15, Chr XV from 662400-663581 [yeast_orfs2] HEM15 SGDID:S0005702, Chr XV from 662401-663582, Verified ORF [yeastflysequest.fasta] Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway; Yfh1p mediates the use of iron by Hem15p [Saccharomyces cerevisiae]gi\|3769 [nrdb_11152004_Chlamydomonas] HEM15 SGDID:S000005702, Chr XV from 662401-663582, Verified ORF, "Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway; Yfh1p med [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] HEM15 SGDID:S000005702, Chr XV from 662402-663583, Verified ORF, "Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway; Yfh1p med [yeast-contam.fasta] HEM15 SGDID:S000005702, Chr XV from 662402-663583, Verified ORF, "Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] HEM15 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ferrochelatase, mitochondrial precursor (Protoheme ferro-lyase) (Heme synthetase) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ferrochelatase precursor (EC 4.99.1.1) [nr-271106-contam.fasta] Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway; Yfh1p mediates the use of iron by Hem15p; Hem15p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] HEM15 SGDID:S000005702, Chr XV from 662402-663583, Verified ORF, "Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway; Yfh1p mediates the use of iron by Hem15p" [yeast-contam_custom.fasta] [chemostatdb.fasta] Ferrochelatase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HEM15 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] HEM15 SGDID:S000005702, Chr XV from 662401-663582, Genome Release 64-1-1, Verified ORF, "Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway" [yeast-030211-contam.fasta] HEM15 SGDID:S000005702, Chr XV from 662401-663582, Genome Release 64-2-1, Verified ORF, "Ferrochelatase; a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway" [20141208_orf_trans.fasta] TR7433\|c0_g1_i1\|g.5189 ORF TR7433\|c0_g1_i1\|g.5189 TR7433\|c0_g1_i1\|m.5189 type:complete len:394 (+) TR7433\|c0_g1_i1:499-1680(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YOR176W SGDID:S000005702, Chr XV from 662401-663582, Genome Release 64-1-1, Verified ORF, "Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_2449\|g.1811 ORF asmbl_2449\|g.1811 asmbl_2449\|m.1811 type:complete len:394 (+) asmbl_2449:491-1672(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_2452\|g.1642 ORF ID=asmbl_2452\|g.1642 ID=asmbl_2452\|m.1642 type:complete len:394 (+) ID=asmbl_2452:492-1673(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	393 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA] mitochondrial inner membrane [ISS][TAS]
Biological Process:	heme biosynthetic process [TAS]
Molecular Function:	ferrochelatase activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLSRTIRTQG SFLRRSQLTI TRSFSVTFNM QNAQKRSPTG IVLMNMGGPS KVEETYDFLY  60
   61 QLFADNDLIP ISAKYQKTIA KYIAKFRTPK IEKQYREIGG GSPIRKWSEY QATEVCKILD 120
  121 KTCPETAPHK PYVAFRYAKP LTAETYKQML KDGVKKAVAF SQYPHFSYST TGSSINELWR 180
  181 QIKALDSERS ISWSVIDRWP TNEGLIKAFS ENITKKLQEF PQPVRDKVVL LFSAHSLPMD 240
  241 VVNTGDAYPA EVAATVYNIM QKLKFKNPYR LVWQSQVGPK PWLGAQTAEI AEFLGPKVDG 300
  301 LMFIPIAFTS DHIETLHEID LGVIGESEYK DKFKRCESLN GNQTFIEGMA DLVKSHLQSN 360
  361 QLYSNQLPLD FALGKSNDPV KDLSLVFGNH EST

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		ADH2 ADH4 ADH5 ADR1 ARC1 AYR1 BGL2 BRX1 CBP3 CBP6 ENP1 FAA1 GAR1 GSP1 HEM15 HHF1, HHF2 HRP1 HTA2 ILV5 KAP104 KTR3 LCD1 MRT4 NAB2 NDE1 NUP84 NUP85 OAC1 PAA1 PET10 PET9 PPE1 PPH21 PPH22 PSD1 PWP1 QCR2 RNQ1 RPC40 RPT4 RTS1 RTS3 SEH1 TAP42 TFG2 TPD3 VMA8 YDL063C YHM2 YMR209C YNL035C
View Details	Qiu et al. (2008)		COX15 HEM15

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	HEM15	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..49] [102..200] [341..393]	PSI-BLAST	1041.218487	Ferrochelatase
2	View Details	[50..101] [201..340]	PSI-BLAST	1041.218487	Ferrochelatase

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.93

Protein Overview: HEM15

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: