Protein Overview: LRO1

General Information:
Name(s) found:	LRO1 / YNR008W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|732207\|sp\|P40345.1\|PDAT_YEAST RecName: Full=Phospholipid:diacylglycerol acyltransferase; Short=PDAT [NCBI NR] gi\|6324335\|ref\|NP_014405.1\| Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase; Lro1p [Saccharomyces cerev [NCBI NR] pir\|\|S45131 probable membrane protein YNR008w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|496725\|emb\|CAA54576.1\| N2042 [Saccharomyces cerevisiae] [NCBI NR] gi\|1302482\|emb\|CAA96285.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae GN=LRO1 PE=1 SV=1 [Swiss-Prot] LRO1 A lecithin cholesterol acyltransferase-like gene, mediates diacylglycerol esterification [mips] Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase [SGD] (P40345) Phospholipid:diacylglycerol acyltransferase (EC 2.3.1.158) (PDAT) [4932.FASTACCERV.txt] Chr XIV from 640393-642378 [hmr_yeast5_DM3-1_pombe.fasta] LRO1, Chr XIV from 640393-642378 [yeast_orfs2] LRO1 SGDID:S0005291, Chr XIV from 640395-642380, Verified ORF [yeastflysequest.fasta] LRO1 SGDID:S0005291, Chr XIV from 640396-642381, Verified ORF [10112005cervSGD.fasta] Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase [Saccharomyces cerevisiae]gi\|1302482\|emb\|CAA96285.1\| u [nrdb_11152004_Chlamydomonas] LRO1 SGDID:S000005291, Chr XIV from 640398-642383, Verified ORF, "Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acylt [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] N2042 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase; Lro1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Phospholipid:diacylglycerol acyltransferase (PDAT) [nr-271106-contam.fasta] LRO1 SGDID:S000005291, Chr XIV from 640399-642384, Verified ORF, "Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acylt [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] LRO1 SGDID:S000005291, Chr XIV from 640398-642383, Verified ORF, "Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase" [yeast-bovrabprots.fasta] [chemostatdb.fasta] LRO1 SGDID:S000005291, Chr XIV from 640399-642384, Verified ORF, "Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase" [SGDyeast.20101221.fasta] Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LRO1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] LRO1 SGDID:S000005291, Chr XIV from 640396-642381, Genome Release 64-1-1, Verified ORF, "Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase" [yeast-030211-contam.fasta] LRO1 SGDID:S000005291, Chr XIV from 640396-642381, Genome Release 64-2-1, Verified ORF, "Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; Lro1p and Dga1p can O-acylate ceramides; putative homolog of human lecithin cholesterol acyltransferase" [20141208_orf_trans.fasta] YNR008W SGDID:S000005291, Chr XIV from 640396-642381, Genome Release 64-1-1, Verified ORF, "Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	661 amino acids
Gene Ontology:
Cellular Component:	endoplasmic reticulum [TAS]
Biological Process:	lipid storage [IDA][IMP] triglyceride biosynthetic process [IDA][IMP]
Molecular Function:	phospholipid:diacylglycerol acyltransferase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGTLFRRNVQ NQKSDSDENN KGGSVHNKRE SRNHIHHQQG LGHKRRRGIS GSAKRNERGK  60
   61 DFDRKRDGNG RKRWRDSRRL IFILGAFLGV LLPFSFGAYH VHNSDSDLFD NFVNFDSLKV 120
  121 YLDDWKDVLP QGISSFIDDI QAGNYSTSSL DDLSENFAVG KQLLRDYNIE AKHPVVMVPG 180
  181 VISTGIESWG VIGDDECDSS AHFRKRLWGS FYMLRTMVMD KVCWLKHVML DPETGLDPPN 240
  241 FTLRAAQGFE STDYFIAGYW IWNKVFQNLG VIGYEPNKMT SAAYDWRLAY LDLERRDRYF 300
  301 TKLKEQIELF HQLSGEKVCL IGHSMGSQII FYFMKWVEAE GPLYGNGGRG WVNEHIDSFI 360
  361 NAAGTLLGAP KAVPALISGE MKDTIQLNTL AMYGLEKFFS RIERVKMLQT WGGIPSMLPK 420
  421 GEEVIWGDMK SSSEDALNNN TDTYGNFIRF ERNTSDAFNK NLTMKDAINM TLSISPEWLQ 480
  481 RRVHEQYSFG YSKNEEELRK NELHHKHWSN PMEVPLPEAP HMKIYCIYGV NNPTERAYVY 540
  541 KEEDDSSALN LTIDYESKQP VFLTEGDGTV PLVAHSMCHK WAQGASPYNP AGINVTIVEM 600
  601 KHQPDRFDIR GGAKSAEHVD ILGSAELNDY ILKIASGNGD LVEPRQLSNL SQWVSQMPFP 660
  661 M

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		AAT2 ACH1 ACO1 AFR1 AHP1 ARC1 ARF1 ATP3 CAR2 CDC33 CLU1 COF1 COR1 CPR1 CYR1 CYS4 EGD1 ERG13 ERG6 FPR1 FRS2 GND1 GND2 GRX1 GUS1 HEF3 HHF1, HHF2 LRO1 LSP1 MET6 NTF2 OLA1 OYE2 PFK1 PIL1 PRM2 RNR2 RSN1 SAH1 SCP160 SEC53 SEN15 SES1 SNU13 SOD1 TEF4 THS1 TIF1, TIF2 TMA19 TSA2 UBA1 VMA4 VMA5 WTM1 YNK1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NAF1	LRO1	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..125]	deduced	N/A	No confident structure predictions are available.
2	View Details	[126..221]	MSA	1.035991	View MSA. No confident structure predictions are available.
3	View Details	[222..392]	ORFEUS	9.89	Lipase
4	View Details	[393..602]	MSA	3.033968	View MSA. No confident structure predictions are available.
5	View Details	[603..661]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2