Protein Overview: AIP1

General Information:
Name(s) found:	AIP1 / YMR092C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|807975\|emb\|CAA89239.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6323739\|ref\|NP_013810.1\| Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; Aip1p [Saccharomyces cerevisiae] [NCBI NR] gi\|1168395\|sp\|P46680.1\|AIP1_YEAST RecName: Full=Actin-interacting protein 1 [NCBI NR] pir\|\|S54468 AIP1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1016760\|gb\|AAA79141.1\| Aip1p [NCBI NR] Actin-interacting protein 1 OS=Saccharomyces cerevisiae GN=AIP1 PE=1 SV=1 [Swiss-Prot] AIP1 Actin cytoskeleton component [mips] Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats [SGD] AIP1, Chr XIII from 451631-453478, reverse complement [yeast_orfs2] Chr XIII from 451631-453478, reverse complement [yeastorf3.fasta] Chr XIII from 453478-451631, reverse complement [yeastorf5.fasta] (P46680) Actin interacting protein 1 [4932.FASTACCERV.txt] AIP1 SGDID:S0004698, Chr XIII from 453478-451631, reverse complement, Verified ORF [yeastflysequest.fasta] Actin cortical patch component; probable binding site on actin lies on front surface of subdomains 3 and 4 [Saccharomyces cerevisiae]gi\|807975\|emb\|CAA89239.1\| unknown [Saccharomyces cerevisiae]gi\|1078591\|pir\|\|S54468 AIP1 protein - yeast (Saccharomyces c [nrdb_11152004_Chlamydomonas] AIP1 SGDID:S000004698, Chr XIII from 453478-451631, reverse complement, Verified ORF, "Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repea [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; Aip1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Aip1p [nr-271106-contam.fasta] Actin-interacting protein 1 [nr-271106-contam.fasta] AIP1 SGDID:S000004698, Chr XIII from 453478-451631, reverse complement, Verified ORF, "Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Actin-interacting protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=AIP1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] AIP1 SGDID:S000004698, Chr XIII from 453479-451632, Genome Release 64-1-1, reverse complement, Verified ORF, "Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats" [yeast-030211-contam.fasta] AIP1 SGDID:S000004698, Chr XIII from 453479-451632, Genome Release 64-2-1, reverse complement, Verified ORF, "Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; inhibits elongation of aged ADP-actin filaments decorated with cofilin to maintain a high level of assembly-competent actin species; required to restrict cofilin localization to cortical patches; putative regulator of cytokinesis; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress" [20141208_orf_trans.fasta] TR8753\|c0_g1_i1\|g.6198 ORF TR8753\|c0_g1_i1\|g.6198 TR8753\|c0_g1_i1\|m.6198 type:complete len:616 (+) TR8753\|c0_g1_i1:287-2134(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YMR092C SGDID:S000004698, Chr XIII from 453479-451632, Genome Release 64-1-1, reverse complement, Verified ORF, "Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_3968\|g.2824 ORF asmbl_3968\|g.2824 asmbl_3968\|m.2824 type:complete len:616 (+) asmbl_3968:287-2134(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_3970\|g.2584 ORF ID=asmbl_3970\|g.2584 ID=asmbl_3970\|m.2584 type:complete len:616 (+) ID=asmbl_3970:287-2134(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	615 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA] actin cortical patch [IDA]
Biological Process:	actin filament depolymerization [IGI][IPI] response to osmotic stress [IMP]
Molecular Function:	protein binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSISLKEII PPQPSTQRNF TTHLSYDPTT NAIAYPCGKS AFVRCLDDGD SKVPPVVQFT  60
   61 GHGSSVVTTV KFSPIKGSQY LCSGDESGKV IVWGWTFDKE SNSVEVNVKS EFQVLAGPIS 120
  121 DISWDFEGRR LCVVGEGRDN FGVFISWDSG NSLGEVSGHS QRINACHLKQ SRPMRSMTVG 180
  181 DDGSVVFYQG PPFKFSASDR THHKQGSFVR DVEFSPDSGE FVITVGSDRK ISCFDGKSGE 240
  241 FLKYIEDDQE PVQGGIFALS WLDSQKFATV GADATIRVWD VTTSKCVQKW TLDKQQLGNQ 300
  301 QVGVVATGNG RIISLSLDGT LNFYELGHDE VLKTISGHNK GITALTVNPL ISGSYDGRIM 360
  361 EWSSSSMHQD HSNLIVSLDN SKAQEYSSIS WDDTLKVNGI TKHEFGSQPK VASANNDGFT 420
  421 AVLTNDDDLL ILQSFTGDII KSVRLNSPGS AVSLSQNYVA VGLEEGNTIQ VFKLSDLEVS 480
  481 FDLKTPLRAK PSYISISPSE TYIAAGDVMG KILLYDLQSR EVKTSRWAFH TSKINAISWK 540
  541 PAEKGANEEE IEEDLVATGS LDTNIFIYSV KRPMKIIKAL NAHKDGVNNL LWETPSTLVS 600
  601 SGADACIKRW NVVLE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		ABP1 AIP1 ARC40 ARP2 BEM1 BUD6 CAP1 CAP2 COF1 CRN1 DLD2 GLK1 LAS17 OYE2 PAN1 PFY1 RVS167 SAC6 SLA1 SLA2 SRV2 TPM1 TPM2 TWF1 VRP1
View Details	Ho Y, et al. (2002)		AIP1 COF1 CYR1 GCN1 KAP114 PHO81 REX2 SRV2 TOS3
View Details	Qiu et al. (2008)		ABP1 ABP140 AIM21 AIP1 ARC40 ARP2 BBC1 BNI1 BSP1 BUD6 CAP1 CAP2 COF1 CRN1 END3 ENT1 ENT2 ENT3 HSK3 IQG1 LAS17 MYO3 MYO5 PAN1 PFY1 RVS161 RVS167 SAC6 SCD5 SCP1 SLA1 SLA2 SRV2 TPM1 TPM2 TWF1 VRP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#27 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps3-5	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	AIP1	SRV2	2	Drees BL, et al. (2001)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	AIP1	Huh WK, et al. (2003)
View Data	AIP1 and SAC6	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..247]	PSI-BLAST	105.519517	Tup1, C-terminal domain
2	View Details	[248..366]	ORFEUS	12.32	Quinohemoprotein amine dehydrogenase B chain
3	View Details	[367..433]	PSI-BLAST	28.0	Groucho/tle1, C-terminal domain
4	View Details	[434..508]	PSI-BLAST	4.0	TolB, C-terminal domain; TolB, N-terminal domain
5	View Details	[509..615]	ORFEUS	14.77	Arp2/3 complex 41 kDa subunit ARPC1

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
actin binding	3.86142918698875	bayes_pls_golite062009
cytoskeletal protein binding	3.1056443224755	bayes_pls_golite062009
binding	2.22722319261713	bayes_pls_golite062009
actin filament binding	2.0735069176589	bayes_pls_golite062009
myosin heavy chain kinase activity	1.24520283111044	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.22384651674093	bayes_pls_golite062009
transporter activity	1.21549336905926	bayes_pls_golite062009
histone methyltransferase activity (H3-K4 specific)	1.14494479608652	bayes_pls_golite062009
protein binding	1.11897341394404	bayes_pls_golite062009
myosin binding	0.989691178611801	bayes_pls_golite062009
tubulin binding	0.968047176592527	bayes_pls_golite062009
	0.92526678064403	bayes_pls_golite062009
microtubule binding	0.834073483714259	bayes_pls_golite062009
substrate-specific transporter activity	0.734793983890004	bayes_pls_golite062009
nucleic acid binding	0.438994634856101	bayes_pls_golite062009
dynein binding	0.41765917405931	bayes_pls_golite062009
transcription regulator activity	0.415449383711249	bayes_pls_golite062009
snoRNA binding	0.347797037104681	bayes_pls_golite062009
structural constituent of cytoskeleton	0.118975415284669	bayes_pls_golite062009
myosin II binding	0.0145748031956598	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.00585398682921312	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2