Protein Overview: PEP3

General Information:
Name(s) found:	PEP3 / YLR148W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323177\|ref\|NP_013249.1\| Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis; Pep3p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|A41943 vacuolar membrane protein PEP3 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|172120\|gb\|AAA34852.1\| PEP3 [Saccharomyces cerevisiae] [NCBI NR] gi\|1360576\|emb\|CAA97720.1\| PEP3 [Saccharomyces cerevisiae] [NCBI NR] gi\|129785\|sp\|P27801.1\|PEP3_YEAST RecName: Full=Vacuolar membrane protein PEP3; AltName: Full=Vacuolar protein sorting-associated protein 18; AltName: Full=Vacuolar morphogenesis protein 8; AltName: Full=Vacuolar protein-targeting protein 18; AltName: Full [NCBI NR] gi\|1262308\|gb\|AAB82382.1\| Pep3p: Vacuolar membrane protein [Saccharomyces cerevisiae] [NCBI NR] Vacuolar membrane protein PEP3 OS=Saccharomyces cerevisiae GN=PEP3 PE=1 SV=1 [Swiss-Prot] PEP3 Vacuolar membrane protein [mips] Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis [SGD] PEP3, Chr XII from 434642-437398 [yeast_orfs2] Chr XII from 434642-437398 [yeastorf3.fasta] (P27801) Vacuolar membrane protein PEP3 [4932.FASTACCERV.txt] PEP3 SGDID:S0004138, Chr XII from 434642-437398, Verified ORF [yeastflysequest.fasta] Pep3p [Saccharomyces cerevisiae]gi\|1360576\|emb\|CAA97720.1\| PEP3 [Saccharomyces cerevisiae]gi\|129785\|sp\|P27801\|PEP3_YEAST Vacuolar membrane protein PEP3gi\|283292\|pir\|\|A41943 vacuolar membrane protein PEP3 - yeast (Saccharomyces cerevisiae)gi\|1262308\|gb [nrdb_11152004_Chlamydomonas] PEP3 SGDID:S000004138, Chr XII from 434642-437398, Verified ORF, "Vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis, forms complex with Pep5p that med [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PEP3 SGDID:S000004138, Chr XII from 434642-437398, Verified ORF, "Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogen [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis, forms complex with Pep5p that mediates protein transport to the vacuole; Pep3p [Saccharomyces cerev [nr-271106-contam.fasta] Vacuolar membrane protein PEP3 (Vacuolar protein sorting-associated protein 18) (Vacuolar morphogenesis protein 8) (Vacuolar protein-targeting protein 18) (Carboxypeptidase Y-deficient protein 3) [nr-271106-contam.fasta] PEP3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Pep3p: Vacuolar membrane protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PEP3 [nr-271106-contam.fasta] PEP3 SGDID:S000004138, Chr XII from 434642-437398, Verified ORF, "Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis" [yeast-bovrabprots.fasta] PEP3 SGDID:S000004138, Chr XII from 434642-437398, Verified ORF, "Vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis, forms complex with Pep5p that mediates protein transport to the vacuole" [yeast-contam_custom.fasta] [chemostatdb.fasta] Vacuolar membrane protein PEP3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PEP3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PEP3 SGDID:S000004138, Chr XII from 434641-437397, Genome Release 64-1-1, Verified ORF, "Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis" [yeast-030211-contam.fasta] PEP3 SGDID:S000004138, Chr XII from 434641-437397, Genome Release 64-2-1, Verified ORF, "Component of CORVET membrane tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis" [20141208_orf_trans.fasta] YLR148W SGDID:S000004138, Chr XII from 434641-437397, Genome Release 64-1-1, Verified ORF, "Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	918 amino acids
Gene Ontology:
Cellular Component:	fungal-type vacuole membrane [IDA] extrinsic to vacuolar membrane [IDA] HOPS complex [IPI]
Biological Process:	response to drug [IMP] vesicle docking during exocytosis [IMP] late endosome to vacuole transport [IGI] telomere maintenance [IMP] vacuole fusion, non-autophagic [IDA] Golgi to endosome transport [IGI]
Molecular Function:	protein binding [IMP][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIKTRIEEVQ LQFLTGNTEL THLKVSNDQL IVTTQRTIYR INLQDPAIVN HFDCPLSKEL  60
   61 ETIMNVHVSP MGSVILIRTN FGRYMLLKDG EFTQLNKIKN LDLSSLHWIN ETTFLMGIKK 120
  121 TPKLYRVELT GKDITTKLWY ENKKLSGGID GIAYWEGSLL LTIKDNILYW RDVTNMKFPL 180
  181 VLPDESEQFE RLKHHAIKKF DSYNGLFAWV TSNGIVFGDL KEKQMEKDPA SNNFGKFLSS 240
  241 SKVLLNFELP DYQNDKDHLI KDIVLTAFHI LLLRKNTVTM VSQLNNDVVF HETIPRHQLT 300
  301 GSNTDSNEKF LGLVRDSVKE TFWCFSNINV FEIIIENEPN SVWNLLVRDN KFDKALSLKG 360
  361 LTVREIESVK LSKAMYLFHT AKDFHSAAQT LGSMKDLSHF GEIALNFLQI KDYNDLNVIL 420
  421 IKQLDNVPWK STQVVLSSWI IWNFMKQLND IELKINTTKP ASTDEDNLLN WNLNLKEKSN 480
  481 ELTKFLESHL EKLDNETVYQ IMSKQNRQNE LLIFASLIND MKFLLSFWID QGNWYESLKI 540
  541 LLTINNHDLV YKYSLILLLN SPEATVSTWM KIKDLDPNKL IPTILKFFTN WQNNSKLITN 600
  601 ISEYPENYSL TYLKWCVREV PKMCNPIVYN SILYMMITDP RNDMILENDI IKFMKSNENK 660
  661 YDLNFQLRLS LKFKKTKTSI FLLTRLNLFE DAIDLALKNN LIDDCKVIVN DEILIEDYKL 720
  721 RKRLWLKIAK HLLLSMKDID IKQLIRTILN DSNEILTIKD LLPFFNEYTT IANLKEELIK 780
  781 FLENHNMKMN EISEDIINSK NLKVEINTEI SKFNEIYRIL EPGKSCDECG KFLQIKKFIV 840
  841 FPCGHCFHWN CIIRVILNSN DYNLRQKTEN FLKAKSKHNL NDLENIIVEK CGLCSDININ 900
  901 KIDQPISIDE TELAKWNE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		PEP3 PEP5 VAM6 VPS16 VPS33 VPS41 VPS8
View Details	(MIPS) Mewes HW, et al. (2004)		PEP3 PEP5 VPS16 VPS33
View Details	Krogan NJ, et al. (2006)		CIT2 PEP3 PRO2 PTC6
View Details	Gavin AC, et al. (2006)		PEP3 PEP5 VAM6 VPS16 VPS33 VPS41 VPS8
View Details	Gavin AC, et al. (2002)		ATP8 LUC7 PEP3 PEP5 SNU71 VAM6 VPS16 VPS33 VPS41 VPS8
View Details	Ho Y, et al. (2002)		PEP3 PTC1 SEC7
View Details	Qiu et al. (2008)		PEP3 PEP5 PEP7 VPS16 VPS33 VPS45 VPS8

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	VAM3	sample: hx3	Hao Xu, et al. (2010)
View Run	VAM3	sample: hx4	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX13 - trans-SNARE complex forms but fusion is blocked.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPT21	PEP3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PEP3	Huh WK, et al. (2003)
View Data	PEP3 and SNF7	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..304]	deduced	N/A	No confident structure predictions are available.
2	View Details	[305..823]	PSI-BLAST	21.30103	Clathrin heavy chain proximal leg segment
3	View Details	[824..918]	deduced	N/A	No confident structure predictions are available.
4	View Details	[816..918]	FFAS03	1.57	No description for 2ecnA was found.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2