Protein Overview: BNR1

General Information:
Name(s) found:	BNR1 / YIL159W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|731888\|sp\|P40450.1\|BNR1_YEAST RecName: Full=BNI1-related protein 1 [NCBI NR] gi\|6322032\|ref\|NP_012107.1\| Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1; Bnr1 [NCBI NR] pir\|\|S48375 hypothetical protein YIL159w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|557764\|emb\|CAA86119.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] BNI1-related protein 1 OS=Saccharomyces cerevisiae GN=BNR1 PE=1 SV=1 [Swiss-Prot] BNR1 Regulator of budding [mips] Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 [SGD] BNR1, Chr IX from 41825-45952 [yeast_orfs2] Chr IX from 41825-45952 [yeastorf3.fasta] (P40450) BNI1 related protein 1 [4932.FASTACCERV.txt] BNR1 SGDID:S0001421, Chr IX from 41825-45952, Verified ORF [yeastflysequest.fasta] Bnr1p [Saccharomyces cerevisiae]gi\|557764\|emb\|CAA86119.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|731888\|sp\|P40450\|BNR1_YEAST BNI1 related protein 1gi\|626303\|pir\|\|S48375 hypothetical protein YIL159w - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] BNR1 SGDID:S000001421, Chr IX from 41825-45952, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, fun [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] BNI1-related protein 1 [nr-271106-contam.fasta] Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1; Bnr1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] BNR1 SGDID:S000001421, Chr IX from 41825-45952, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1" [yeast-bovrabprots.fasta] [chemostatdb.fasta] BNI1-related protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BNR1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] BNR1 SGDID:S000001421, Chr IX from 41825-45952, Genome Release 64-1-1, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1" [yeast-030211-contam.fasta] BNR1 SGDID:S000001421, Chr IX from 41825-45952, Genome Release 64-2-1, Verified ORF, "Formin; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1" [20141208_orf_trans.fasta] YIL159W SGDID:S000001421, Chr IX from 41825-45952, Genome Release 64-1-1, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1375 amino acids
Gene Ontology:
Cellular Component:	cellular bud neck contractile ring [IDA]
Biological Process:	axial cellular bud site selection [IMP] response to osmotic stress [IGI] actin filament organization [TAS]
Molecular Function:	cytoskeletal protein binding [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDSSPNKKTY RYPRRSLSLH ARDRVSEARK LEELNLNDGL VAAGLQLVGV ALEKQGTGSH  60
   61 IYMKQKNFSA NDVSSSPMVS EEVNGSEMDF NPKCMPQDAS LVERMFDELL KDGTFFWGAA 120
  121 YKNLQNISLR RKWLLICKIR SSNHWGKKKV TSSTTYSTHL ATNELAENAH FLDGLVRNLS 180
  181 TGGMKLSKAL YKLEKFLRKQ SFLQLFLKDE IYLTTLIEKT LPLISKELQF VYLRCFKILM 240
  241 NNPLARIRAL HSEPLIRWFT ELLTDQNSNL KCQLLSMELL LLLTYVEGST GCELIWDQLS 300
  301 ILFTDWLEWF DKILADDIAI HSSLYLNWNQ LKIDYSTTFL LLINSILQGF NNKTALEILN 360
  361 FLKKNNIHNT ITFLELAYKD DPNSVVIMEQ IKQFKSKESA IFDSMIKTTN DTNSLHPTKD 420
  421 IARIESEPLC LENCLLLKAK DSPVEAPINE IIQSLWKILD SQKPYSESIK LLKLINSLLF 480
  481 YLIDSFQVST NPSFDETLES AENVDYVFQD SVNKLLDSLQ SDEIARRAVT EIDDLNAKIS 540
  541 HLNEKLNLVE NHDKDHLIAK LDESESLISL KTKEIENLKL QLKATKKRLD QITTHQRLYD 600
  601 QPPSLASSNL SIAGSIIKNN SHGNIIFQNL AKKQQQQQKI SLPKRSTSLL KSKRVTSLSS 660
  661 YLTDANNENE SQNESEDKSK DSLFQRSTST INFNIPSMKN ITNMQNVSLN SILSELEFSN 720
  721 SLGTQPNYQS SPVLSSVSSS PKLFPRLSSD SLDNGIQLVP EVVKLPQLPP PPPPPPPPPL 780
  781 PQSLLTEAEA KPDGVSCIAA PAPPPLPDLF KTKTCGAVPP PPPPPPLPES LSMNKGPSNH 840
  841 DLVTPPAPPL PNGLLSSSSV SINPTTTDLK PPPTEKRLKQ IHWDKVEDIK DTLWEDTFQR 900
  901 QETIKELQTD GIFSQIEDIF KMKSPTKIAN KRNAESSIAL SSNNGKSSNE LKKISFLSRD 960
  961 LAQQFGINLH MFSQLSDMEF VMKVLNCDND IVQNVNILKF FCKEELVNIP KSMLNKYEPY1020
 1021 SQGKDGKAVS DLQRADRIFL ELCINLRFYW NARSKSLLTL STYERDYYDL IFKLQKIDDA1080
 1081 ISHLNRSPKF KSLMFIITEI GNHMNKRIVK GIKLKSLTKL AFVRSSIDQN VSFLHFIEKV1140
 1141 IRIKYPDIYG FVDDLKNIED LGKISLEHVE SECHEFHKKI EDLVTQFQVG KLSKEENLDP1200
 1201 RDQIIKKVKF KINRAKTKSE LLIGQCKLTL IDLNKLMKYY GEDPKDKESK NEFFQPFIEF1260
 1261 LAMFKKCAKE NIEKEEMERV YEQRKSLLDM RTSSNKKSNG SDENDGEKVN RDAVDLLISK1320
 1321 LREVKKDPEP LRRRKSTKLN EIAINVHEGD VKTRKDEDHV LLERTHAMLN DIQNI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BNR1 JJJ2 NOP56 NOP9
View Details	Ho Y, et al. (2002)		ADR1 BMH1 BNR1 BOI2 CSR2 CYK3 GSY2 KCS1 NTH1 REG1 SOK1 STU1 SVL3 YFR017C YIL028W
View Details	Qiu et al. (2008)		BEM1 BNI1 BNR1 BUD3 BUD6 ELM1 HOF1 IQG1 LAS17 MLC2 MYO1 MYO2 MYO4 PEA2 PFY1 PXL1 SPA2 SPH1 TPM2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NSL1	No Comments	Hazbun TR, et al. (2003)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#34 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	MET30	BNR1	2	Unpublished Fields Lab Data
View Screen	PRP40	BNR1	2	Unpublished Fields Lab Data
View Screen	PRP40	BNR1	2	Unpublished Fields Lab Data
View Screen	SST2	BNR1	2	Unpublished Fields Lab Data
View Screen	SPO21	BNR1	2	Unpublished Fields Lab Data
View Screen	PFY1	BNR1	2	Drees BL, et al. (2001)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BNR1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..162]	MSA	1.009996	View MSA. No confident structure predictions are available.
2	View Details	[163..291]	ORFEUS	8.79	beta-Catenin
3	View Details	[292..393]	ORFEUS	8.79	beta-Catenin
4	View Details	[394..522]	ORFEUS	8.79	beta-Catenin
5	View Details	[523..603]	ORFEUS	8.79	beta-Catenin
6	View Details	[604..665]	PSI-BLAST	6.154902	Dynamin
7	View Details	[666..867]	ORFEUS	11.94	Fibrinogen
8	View Details	[868..951]	deduced	N/A	No confident structure predictions are available.
9	View Details	[952..999] [1069..1122]	ORFEUS	7.79	DNA-directed RNA polymerase alpha(2), beta, beta-prime and omega subunits
10	View Details	[1000..1068] [1123..1200]	ORFEUS	7.79	DNA-directed RNA polymerase alpha(2), beta, beta-prime and omega subunits
11	View Details	[1201..1323]	ORFEUS	7.79	DNA-directed RNA polymerase alpha(2), beta, beta-prime and omega subunits
12	View Details	[1324..1375]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
actin binding	3.34487439883854	bayes_pls_golite062009
binding	2.6374633159018	bayes_pls_golite062009
cytoskeletal protein binding	2.61566088685247	bayes_pls_golite062009
protein transporter activity	2.56209426863971	bayes_pls_golite062009
protein binding	1.73688475473996	bayes_pls_golite062009
protein transmembrane transporter activity	0.856755253686635	bayes_pls_golite062009
transporter activity	0.825258162383893	bayes_pls_golite062009
enzyme regulator activity	0.698613639309592	bayes_pls_golite062009
substrate-specific transporter activity	0.500493982824973	bayes_pls_golite062009
nucleic acid binding	0.345785895390889	bayes_pls_golite062009
signal sequence binding	0.27824906981293	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.248376041999546	bayes_pls_golite062009
pyrophosphatase activity	0.20813279031582	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.197447861665299	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.196910953077117	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	0.0332743336452223	bayes_pls_golite062009
GTPase regulator activity	0.0268373969988844	bayes_pls_golite062009
guanyl ribonucleotide binding	0.00234756529236324	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
actin binding	4.76659069034376	bayes_pls_golite062009
cytoskeletal protein binding	3.48744511411671	bayes_pls_golite062009
actin filament binding	2.99963220150463	bayes_pls_golite062009
binding	2.86147407357315	bayes_pls_golite062009
protein binding	2.12168271674167	bayes_pls_golite062009
GTPase regulator activity	1.66986263418539	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	1.64990514605397	bayes_pls_golite062009
small GTPase regulator activity	1.2550809703725	bayes_pls_golite062009
enzyme regulator activity	1.25381967078645	bayes_pls_golite062009
profilin binding	0.91195854193678	bayes_pls_golite062009
small GTPase binding	0.8507658747425	bayes_pls_golite062009
GTPase binding	0.82889812790119	bayes_pls_golite062009
guanyl-nucleotide exchange factor activity	0.745321902095093	bayes_pls_golite062009
structural constituent of cytoskeleton	0.35466574267734	bayes_pls_golite062009
structural constituent of muscle	0.276130226777911	bayes_pls_golite062009
pyrophosphatase activity	0.205756341419244	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.195095639672769	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.19455798195809	bayes_pls_golite062009
phosphatidylinositol-4,5-bisphosphate binding	0.0524318525517602	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2