Protein Overview: GPA1

General Information:
Name(s) found:	GPA1 / YHR005C [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	GTP-binding alpha subunit of the heterotrimeric G protein that couples to pheromone receptors; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome [SGD]
Organism:	Saccharomyces cerevisiae
Length:	472 amino acids
Gene Ontology:
Cellular Component:	endosome [IDA] heterotrimeric G-protein complex [IDA][IMP][IPI] plasma membrane [IDA]
Biological Process:	response to drug [IMP] heterotrimeric G-protein complex cycle [IMP] inositol lipid-mediated signaling [IMP] pheromone-dependent signal transduction involved in conjugation with cellular fusion [IMP]
Molecular Function:	GTPase activity [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGCTVSTQTI GDESDPFLQN KRANDVIEQS LQLEKQRDKN EIKLLLLGAG ESGKSTVLKQ  60
   61 LKLLHQGGFS HQERLQYAQV IWADAIQSMK ILIIQARKLG IQLDCDDPIN NKDLFACKRI 120
  121 LLKAKALDYI NASVAGGSDF LNDYVLKYSE RYETRRRVQS TGRAKAAFDE DGNISNVKSD 180
  181 TDRDAETVTQ NEDADRNNSS RINLQDICKD LNQEGDDQMF VRKTSREIQG QNRRNLIHED 240
  241 IAKAIKQLWN NDKGIKQCFA RSNEFQLEGS AAYYFDNIEK FASPNYVCTD EDILKGRIKT 300
  301 TGITETEFNI GSSKFKVLDA GGQRSERKKW IHCFEGITAV LFVLAMSEYD QMLFEDERVN 360
  361 RMHESIMLFD TLLNSKWFKD TPFILFLNKI DLFEEKVKSM PIRKYFPDYQ GRVGDAEAGL 420
  421 KYFEKIFLSL NKTNKPIYVK RTCATDTQTM KFVLSAVTDL IIQQNLKKIG II

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		GPA1 STE18 STE4
View Details	Ho Y, et al. (2002)		AHA1 ARP2 CCT2 CCT3 CCT5 CCT6 GCD11 GPA1 GPA2 RNQ1 STE4 TCP1 YHR033W
View Details	Qiu et al. (2008)		GPA1 STE18 STE4

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	GPA1	YHL026C	2	Unpublished Fields Lab Data
View Screen	GPA1	YBR116C	2	Unpublished Fields Lab Data
View Screen	GPA1	ATG14	2	Unpublished Fields Lab Data
View Screen	GPA1	NAT4	2	Unpublished Fields Lab Data
View Screen	GPA1	GEP4	2	Unpublished Fields Lab Data
View Screen	GPA1	GAC1	2	Unpublished Fields Lab Data
View Screen	GPA1	CSN9	2	Unpublished Fields Lab Data
View Screen	GPA1	MDM31	2	Unpublished Fields Lab Data
View Screen	GPA1	SCP160	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GPA1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..146]	Pfam	5.823909	G-protein alpha subunit No confident structure predictions are available.
2	View Details	[147..472]	PSI-BLAST	1559.0	No description for 1scgA_ was found.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
GTPase activity	4.92902654551215	bayes_pls_golite062009
microtubule motor activity	3.80749143568847	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	3.4837867129694	bayes_pls_golite062009
ion transmembrane transporter activity	3.35436118751376	bayes_pls_golite062009
transporter activity	3.10463051760206	bayes_pls_golite062009
cation transmembrane transporter activity	3.08390071341252	bayes_pls_golite062009
opioid receptor binding	3.0590320138875	bayes_pls_golite062009
mu-type opioid receptor binding	3.0590320138875	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.95079405147356	bayes_pls_golite062009
pyrophosphatase activity	2.87427509025394	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.85499564954033	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.85178667290149	bayes_pls_golite062009
primary active transmembrane transporter activity	2.61775212528604	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.61467977505707	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.57552435627908	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.57107347424517	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.57054427875021	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
microfilament motor activity	2.50080241360447	bayes_pls_golite062009
ATPase activity, coupled	2.43862163407966	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	2.40292717311506	bayes_pls_golite062009
ATPase activity	2.39535714892224	bayes_pls_golite062009
motor activity	2.32425703346903	bayes_pls_golite062009
metal ion transmembrane transporter activity	2.26365379756656	bayes_pls_golite062009
binding	2.21604035604783	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	2.1115517127743	bayes_pls_golite062009
substrate-specific transporter activity	1.91091452720653	bayes_pls_golite062009
G-protein coupled receptor activity	1.63949223587737	bayes_pls_golite062009
G-protein-coupled receptor binding	1.59603802863121	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	1.58971273330352	bayes_pls_golite062009
hydrolase activity	1.57549510726226	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	1.4343912580786	bayes_pls_golite062009
actin-dependent ATPase activity	1.24861649961991	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of ions	1.23799860254751	bayes_pls_golite062009
cation channel activity	0.997064416395892	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
di-, tri-valent inorganic cation transmembrane transporter activity	0.767424210804186	bayes_pls_golite062009
voltage-gated cation channel activity	0.688632087597536	bayes_pls_golite062009
molecular transducer activity	0.683309012097319	bayes_pls_golite062009
signal transducer activity	0.683309012097319	bayes_pls_golite062009
transmembrane receptor activity	0.661834961742057	bayes_pls_golite062009
guanyl nucleotide binding	0.656009904481476	bayes_pls_golite062009
guanyl ribonucleotide binding	0.64416740142962	bayes_pls_golite062009
GTP binding	0.643084645864898	bayes_pls_golite062009
calcium channel activity	0.636216614902039	bayes_pls_golite062009
receptor activity	0.633514002718998	bayes_pls_golite062009
voltage-gated channel activity	0.562664200036023	bayes_pls_golite062009
voltage-gated ion channel activity	0.559798751382976	bayes_pls_golite062009
plus-end-directed microtubule motor activity	0.471293810042041	bayes_pls_golite062009
anion transmembrane transporter activity	0.43332053110728	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
drug transmembrane transporter activity	0.38711726712063	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
potassium channel activity	0.329290830676079	bayes_pls_golite062009
gated channel activity	0.299411774392227	bayes_pls_golite062009
transition metal ion transmembrane transporter activity	0.248918847596573	bayes_pls_golite062009
nucleic acid binding	0.194779852865789	bayes_pls_golite062009
metabotropic serotonin receptor binding	0.178039386254807	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
ion channel activity	0.154779598791225	bayes_pls_golite062009
passive transmembrane transporter activity	0.134817721656507	bayes_pls_golite062009
channel activity	0.134817721656507	bayes_pls_golite062009
substrate-specific channel activity	0.126911677161968	bayes_pls_golite062009
voltage-gated calcium channel activity	0.0789800146503108	bayes_pls_golite062009
cation-transporting ATPase activity	0.0536898657672154	bayes_pls_golite062009
inorganic anion transmembrane transporter activity	0.0122470146764899	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.95

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2