Protein Overview: NUM1

General Information:
Name(s) found:	NUM1 / YDR150W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|899399\|emb\|CAA90372.1\| Num1p [Saccharomyces cerevisiae] [NCBI NR] gi\|6320353\|ref\|NP_010434.1\| Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex; Num1p [Saccharomyces cerevisiae] [NCBI NR] gi\|33860190\|sp\|Q00402.2\|NUM1_YEAST RecName: Full=Nuclear migration protein NUM1 [NCBI NR] gi\|2132366\|pir\|\|S57976 nuclear migration protein NUM1 - yeast (Saccharomyces cerevisiae) [NCBI NR] Nuclear migration protein NUM1 OS=Saccharomyces cerevisiae GN=NUM1 PE=1 SV=2 [Swiss-Prot] NUM1 Nuclear migration protein [mips] Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex [SGD] NUM1, Chr IV from 755621-763867 [yeast_orfs2] Chr IV from 755621-763867 [yeastorf3.fasta] (Q00402) Nuclear migration protein NUM1 [4932.FASTACCERV.txt] NUM1 SGDID:S0002557, Chr IV from 755622-763868, Verified ORF [yeastflysequest.fasta] NUM1 SGDID:S0002557, Chr IV from 755623-763869, Verified ORF [10112005cervSGD.fasta] Num1p [Saccharomyces cerevisiae]gi\|899399\|emb\|CAA90372.1\| Num1p [Saccharomyces cerevisiae]gi\|2132366\|pir\|\|S57976 nuclear migration protein NUM1 - yeast (Saccharomyces cerevisiae)gi\|33860190\|sp\|Q00402\|NUM1_YEAST Nuclear migration protein NUM1 [nrdb_11152004_Chlamydomonas] NUM1 SGDID:S000002557, Chr IV from 755623-763869, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NUM1 SGDID:S000002557, Chr IV from 755625-763871, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" [yeast-contam.fasta] NUM1 SGDID:S000002557, Chr IV from 755626-763872, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Nuclear migration protein NUM1 [nr-271106-contam.fasta] nuclear migration protein NUM1 - yeast (Saccharomyces cerevisiae) [nr-271106-contam.fasta] Num1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex; Num1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Nuclear migration protein NUM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NUM1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NUM1 SGDID:S000002557, Chr IV from 755628-763874, Genome Release 64-1-1, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" [yeast-030211-contam.fasta] NUM1 SGDID:S000002557, Chr IV from 755628-763874, Genome Release 64-2-1, Verified ORF, "Protein required for nuclear migration; component of the mitochondria-ER-cortex-ancor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and motochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" [20141208_orf_trans.fasta] YDR150W SGDID:S000002557, Chr IV from 755628-763874, Genome Release 64-1-1, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	2748 amino acids
Gene Ontology:
Cellular Component:	cellular bud tip [IDA] cell cortex [IDA] mitochondrion [IDA ]
Biological Process:	mitochondrion inheritance [IGI] nuclear migration along microtubule [IMP] microtubule cytoskeleton organization [IMP] mitochondrial fission [IMP]
Molecular Function:	tubulin binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSHNNRHKKN NDKDSSAGQY ANSIDNSLSQ ESVSTNGVTR MANLKADECG SGDEGDKTKR  60
   61 FSISSILSKR ETKDVLPEFA GSSSHNGVLT ANSSKDMNFT LELSENLLVE CRKLQSSNEA 120
  121 KNEQIKSLKQ IKESLSDKIE ELTNQKKSFM KELDSTKDLN WDLESKLTNL SMECRQLKEL 180
  181 KKKTEKSWND EKESLKLLKT DLEILTLTKN GMENDLSSQK LHYDKEISEL KERILDLNNE 240
  241 NDRLLISVSD LTSEINSLQS NRTERIKIQK QLDDAKASIS SLKRKVQKKY YQKQHTSDTT 300
  301 VTSDPDSEGT TSEEDIFDIV IEIDHMIETG PSVEDISEDL VKKYSEKNNM ILLSNDSYKN 360
  361 LLQKSESASK PKDDELMTKE VAENLNMIAL PNDDNYSKKE FSLESHIKYL EASGYKVLPL 420
  421 EEFENLNESL SNPSYNYLKE KLQALKKIPI DQSTFNLLKE PTIDFLLPLT SKIDCLIIPT 480
  481 KDYNDLFESV KNPSIEQMKK CLEAKNDLQS NICKWLEERN GCKWLSNDLY FSMVNKIETP 540
  541 SKQYLSDKAK EYDQVLIDTK ALEGLKNPTI DFLREKASAS DYLLLKKEDY VSPSLEYLVE 600
  601 HAKATNHHLL SDSAYEDLVK CKENPDMEFL KEKSAKLGHT VVSNEAYSEL EKKLEQPSLE 660
  661 YLVEHAKATN HHLLSDSAYE DLVKCKENPD MEFLKEKSAK LGHTVVSNEA YSELQRKYSE 720
  721 LEKEVEQPSL AYLVEHAKAT DHHLLSDSAY EDLVKCKENP DVEFLKEKSA KLGHTVVSSE 780
  781 EYSELQRKYS ELEKEVEQPS LAYLVEHAKA TDHHLLSDSA YEELVKCKEN PDMEFLKEKS 840
  841 AKLGHTVVSN EAYSELEKKL EQPSLAYLVE HAKATDHHLL SDSAYEDLVK CKENSDVEFL 900
  901 KEKSAKLGHT VVSNEAYSEL EKKLEQPSLA YLVEHAKATD HHLLSDSAYE DLVKCKENPD 960
  961 MEFLKEKSAK LGHTVVSNEA YSELEKKLEQ PSLEYLVEHA KATNHHLLSD SAYEDLVKCK1020
 1021 ENPDMEFLKE KSAKLGHTVV SNEAYSELEK KLEQPSLEYL VEHAKATNHH LLSDSAYEEL1080
 1081 VKCKENPDVE FLKEKSAKLG HTVVSNEAYS ELEKKLEQPS LEYLVEHAKA TNHHLLSDSA1140
 1141 YEELVKCKEN PDVEFLKEKS AKLGHTVVSN EAYSELEKKL EQPSLAYLVE HAKATDHHLL1200
 1201 SDSAYEDLVK CKENPDVEFL KEKSAKLGHT VVSNEAYSEL EKKLEQPSLA YLVEHAKATD1260
 1261 HHLLSDSAYE DLVKCKENPD MEFLKEKSAK LGHTVVSNEA YSELEKKLEQ PSLEYLVEHA1320
 1321 KATNHHLLSD SAYEDLVKCK ENPDMEFLKE KSAKLGHTVV SNKEYSELEK KLEQPSLEYL1380
 1381 VKHAEQIQSK IISISDFNTL ANPSMEDMAS KLQKLEYQIV SNDEYIALKN TMEKPDVELL1440
 1441 RSKLKGYHII DTTTYNELVS NFNSPTLKFI EEKAKSKGYR LIEPNEYLDL NRIATTPSKE1500
 1501 EIDNFCKQIG CYALDSKEYE RLKNSLENPS KKFIEENAAL LDLVLVDKTE YQAMKDNASN1560
 1561 KKSLIPSTKA LDFVTMPAPQ LASAEKSSLQ KRTLSDIENE LKALGYVAIR KENLPNLEKP1620
 1621 IVDNASKNDV LNLCSKFSLV PLSTEEYDNM RKEHTKILNI LGDPSIDFLK EKCEKYQMLI1680
 1681 ISKHDYEEKQ EAIENPGYEF ILEKASALGY ELVSEVELDR MKQMIDSPDI DYMQEKAARN1740
 1741 EMVLLRNEEK EALQKKIEYP SLTFLIEKAA GMNKILVDQI EYDETIRKCN HPTRMELEES1800
 1801 CHHLNLVLLD QNEYSTLREP LENRNVEDLI NTLSKLNYIA IPNTIYQDLI GKYENPNFDY1860
 1861 LKDSLNKMDY VAISRQDYEL MVAKYEKPQL DYLKISSEKI DHIVVPLSEY NLMVTNYRNP1920
 1921 SLSYLKEKAV LNNHILIKED DYKNILAVSE HPTVIHLSEK ASLLNKVLVD KDDFATMSRS1980
 1981 IEKPTIDFLS TKALSMGKIL VNESTHKRNE KLLSEPDSEF LTMKAKEQGL IIISEKEYSE2040
 2041 LRDQIDRPSL DVLKEKAAIF DSIIVENIEY QQLVNTTSPC PPITYEDLKV YAHQFGMELC2100
 2101 LQKPNKLSGA ERAERIDEQS INTTSSNSTT TSSMFTDALD DNIEELNRVE LQNNEDYTDI2160
 2161 ISKSSTVKDA TIFIPAYENI KNSAEKLGYK LVPFEKSNIN LKNIEAPLFS KDNDDTSVAS2220
 2221 SIDLDHLSRK AEKYGMTLIS DQEFEEYHIL KDNAVNLNGG MEEMNNPLSE NQNLAAKTTN2280
 2281 TAQEGAFQNT VPHNDMDNEE VEYGPDDPTF TVRQLKKPAG DRNLILTSRE KTLLSRDDNI2340
 2341 MSQNEAVYGD DISDSFVDES QEIKNDVDII KTQAMKYGML CIPESNFVGA SYASAQDMSD2400
 2401 IVVLSASYYH NLMSPEDMKW NCVSNEELQA EVKKRGLQIA LTTKEDKKGQ ATASKHEYVS2460
 2461 HKLNNKTSTV STKSGAKKGL AEAAATTAYE DSESHPQIEE QSHRTNHHKH HKRQQSLNSN2520
 2521 STSKTTHSSR NTPASRRDIV ASFMSRAGSA SRTASLQTLA SLNEPSIIPA LTQTVIGEYL2580
 2581 FKYYPRLGPF GFESRHERFF WVHPYTLTLY WSASNPILEN PANTKTKGVA ILGVESVTDP2640
 2641 NPYPTGLYHK SIVVTTETRT IKFTCPTRQR HNIWYNSLRY LLQRNMQGIS LEDIADDPTD2700
 2701 NMYSGKIFPL PGENTKSSSK RLSASRRSVS TRSLRHRVPQ SRSFGNLR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		GNA1 NUM1 PUP2
View Details	Gavin AC, et al. (2006)		GFA1 MSN5 NUM1
View Details	Gavin AC, et al. (2002)		FKS1 NUM1 OPI1 OSH2 PIL1 RPN10 SCS2 STT4 SWH1 YAR044W
View Details	Ho Y, et al. (2002)		MER1 NUM1 PAA1 PTC4 SEF1 SKT5 SPT16 SYF1 TPS1 VHS1
View Details	Qiu et al. (2008)		BIK1 DYN1 NUM1 STU2 VRP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#34 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	NUM1	Huh WK, et al. (2003)

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: NUM1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: