Protein Overview: GLK1

General Information:
Name(s) found:	GLK1 / YCL040W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|JT0482 glucokinase (EC 2.7.1.2) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6319809\|ref\|NP_009890.1\| Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; Glk1p [Sacc [NCBI NR] gi\|577521\|gb\|AAA53536.1\| glucokinase [Saccharomyces cerevisiae] [NCBI NR] gi\|5323\|emb\|CAA42376.1\| aldohexose specific glucokinase [Saccharomyces cerevisiae] [NCBI NR] gi\|123899\|sp\|P17709.1\|HXKG_YEAST RecName: Full=Glucokinase-1; AltName: Full=Glucose kinase 1; Short=GLK-1 [NCBI NR] Glucokinase-1 OS=Saccharomyces cerevisiae GN=GLK1 PE=1 SV=1 [Swiss-Prot] GLK1 Aldohexose specific glucokinase [mips] Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources [SGD] GLK1, Chr III from 50838-52340 [yeast_orfs2] Chr III from 50838-52340 [yeastorf3.fasta] (P17709) Glucokinase (EC 2.7.1.2) (Glucose kinase) (GLK) [4932.FASTACCERV.txt] GLK1 SGDID:S0000545, Chr III from 50838-52340, Verified ORF [yeastflysequest.fasta] Glk1p [Saccharomyces cerevisiae]gi\|5323\|emb\|CAA42376.1\| aldohexose specific glucokinase [Saccharomyces cerevisiae]gi\|123899\|sp\|P17709\|HXKG_YEAST Glucokinase (Glucose kinase) (GLK)gi\|83035\|pir\|\|JT0482 glucokinase (EC 2.7.1.2) - yeast (Saccharomyces cere [nrdb_11152004_Chlamydomonas] GLK1 SGDID:S000000545, Chr III from 50838-52340, Verified ORF, "Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fer [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] glucokinase [nr-271106-contam.fasta] aldohexose specific glucokinase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; Glk1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Glucokinase GLK1 (Glucose kinase) (GLK) [nr-271106-contam.fasta] GLK1 SGDID:S000000545, Chr III from 50838-52340, Verified ORF, "Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Glucokinase-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GLK1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GLK1 SGDID:S000000545, Chr III from 50838-52340, Genome Release 64-1-1, Verified ORF, "Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources" [yeast-030211-contam.fasta] GLK1 SGDID:S000000545, Chr III from 50838-52340, Genome Release 64-2-1, Verified ORF, "Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR8405\|c0_g1_i1\|g.5815 ORF TR8405\|c0_g1_i1\|g.5815 TR8405\|c0_g1_i1\|m.5815 type:complete len:501 (+) TR8405\|c0_g1_i1:511-2013(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR8405\|c0_g2_i1\|g.5817 ORF TR8405\|c0_g2_i1\|g.5817 TR8405\|c0_g2_i1\|m.5817 type:complete len:501 (+) TR8405\|c0_g2_i1:511-2013(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR8405\|c0_g2_i1\|g.5818 ORF TR8405\|c0_g2_i1\|g.5818 TR8405\|c0_g2_i1\|m.5818 type:complete len:501 (+) TR8405\|c0_g2_i1:2586-4088(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR5766\|c0_g1_i1\|g.4047 ORF TR5766\|c0_g1_i1\|g.4047 TR5766\|c0_g1_i1\|m.4047 type:complete len:501 (+) TR5766\|c0_g1_i1:255-1757(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YCL040W SGDID:S000000545, Chr III from 50838-52340, Genome Release 64-1-1, Verified ORF, "Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_9096\|g.6661 ORF asmbl_9096\|g.6661 asmbl_9096\|m.6661 type:complete len:501 (+) asmbl_9096:253-1755(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_9196\|g.5591 ORF ID=asmbl_9196\|g.5591 ID=asmbl_9196\|m.5591 type:complete len:501 (+) ID=asmbl_9196:254-1756(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_9293\|g.5633 ORF ID=asmbl_9293\|g.5633 ID=asmbl_9293\|m.5633 type:complete len:501 (+) ID=asmbl_9293:510-2012(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	500 amino acids
Gene Ontology:
Cellular Component:	cytosol [IDA]
Biological Process:	glycolysis [IDA] mannose metabolic process [IDA] glucose import [IGI] glucose metabolic process [IMP]
Molecular Function:	glucokinase activity [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFDDLHKAT ERAVIQAVDQ ICDDFEVTPE KLDELTAYFI EQMEKGLAPP KEGHTLASDK  60
   61 GLPMIPAFVT GSPNGTERGV LLAADLGGTN FRICSVNLHG DHTFSMEQMK SKIPDDLLDD 120
  121 ENVTSDDLFG FLARRTLAFM KKYHPDELAK GKDAKPMKLG FTFSYPVDQT SLNSGTLIRW 180
  181 TKGFRIADTV GKDVVQLYQE QLSAQGMPMI KVVALTNDTV GTYLSHCYTS DNTDSMTSGE 240
  241 ISEPVIGCIF GTGTNGCYME EINKITKLPQ ELRDKLIKEG KTHMIINVEW GSFDNELKHL 300
  301 PTTKYDVVID QKLSTNPGFH LFEKRVSGMF LGEVLRNILV DLHSQGLLLQ QYRSKEQLPR 360
  361 HLTTPFQLSS EVLSHIEIDD STGLRETELS LLQSLRLPTT PTERVQIQKL VRAISRRSAY 420
  421 LAAVPLAAIL IKTNALNKRY HGEVEIGCDG SVVEYYPGFR SMLRHALALS PLGAEGERKV 480
  481 HLKIAKDGSG VGAALCALVA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		ABP1 AIP1 ARC40 ARP2 BEM1 BUD6 CAP1 CAP2 COF1 CRN1 DLD2 GLK1 LAS17 OYE2 PAN1 PFY1 RVS167 SAC6 SLA1 SLA2 SRV2 TPM1 TPM2 TWF1 VRP1
View Details	Krogan NJ, et al. (2006)		BUD21 GLK1 NOP58 PRP4 RRP9
View Details	Qiu et al. (2008)		ABP1 ARP2 ATG19 GLK1 GPM2 HXK1 HXK2 PFK1 PFK2 PFK26 PGM2 SLA2 SOL4 SYP1 TPS1 VRP1 XKS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	LOS1	GLK1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	GLK1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..22] [332..412]	PSI-BLAST	863.9691	Hexokinase
2	View Details	[23..331] [413..500]	PSI-BLAST	863.9691	Hexokinase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
kinase activity	2.81480196738665	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	2.66634930184926	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	2.47806699612578	bayes_pls_golite062009
structural molecule activity	2.37892721989886	bayes_pls_golite062009
carbohydrate kinase activity	2.33338276303563	bayes_pls_golite062009
binding	2.11855816699924	bayes_pls_golite062009
transcription regulator activity	2.09111975841662	bayes_pls_golite062009
protein binding	2.00711904074672	bayes_pls_golite062009
nucleic acid binding	1.84294882554466	bayes_pls_golite062009
hexokinase activity	1.70516840340504	bayes_pls_golite062009
DNA binding	1.70014394990425	bayes_pls_golite062009
cytoskeletal protein binding	1.69431775343358	bayes_pls_golite062009
structural constituent of cytoskeleton	1.58791290104307	bayes_pls_golite062009
glucose binding	1.58717365638543	bayes_pls_golite062009
transferase activity	1.58048472128722	bayes_pls_golite062009
transcription activator activity	1.35676801272576	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	1.30214785132007	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	1.30181101672725	bayes_pls_golite062009
pyrophosphatase activity	1.27871475799533	bayes_pls_golite062009
myosin binding	1.26046360733002	bayes_pls_golite062009
nucleoside-triphosphatase activity	1.18894708772738	bayes_pls_golite062009
transcription factor activity	1.06678984888387	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.908385694796738	bayes_pls_golite062009
mannokinase activity	0.766256764215041	bayes_pls_golite062009
actin binding	0.742062735414161	bayes_pls_golite062009
protein kinase activity	0.659672620542945	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.574998559133169	bayes_pls_golite062009
hydrolase activity	0.474893704700898	bayes_pls_golite062009
glucokinase activity	0.47468456642723	bayes_pls_golite062009
sugar binding	0.44352406324938	bayes_pls_golite062009
adenyl nucleotide binding	0.426216374317549	bayes_pls_golite062009
ATP binding	0.39408967688855	bayes_pls_golite062009
adenyl ribonucleotide binding	0.358452809509562	bayes_pls_golite062009
ATPase activity	0.338933039062396	bayes_pls_golite062009
catalytic activity	0.296484721581333	bayes_pls_golite062009
monosaccharide binding	0.20435276425198	bayes_pls_golite062009
purine nucleotide binding	0.173637392484485	bayes_pls_golite062009
nucleotide binding	0.168207023557983	bayes_pls_golite062009
ribonucleotide binding	0.104339549630743	bayes_pls_golite062009
purine ribonucleotide binding	0.101742118226005	bayes_pls_golite062009
chromatin binding	0.0489277194984092	bayes_pls_golite062009
unfolded protein binding	0.0304569608189402	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.95

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2