Cloning of genes that complement yeast hexokinase and glucokinase mutants

J Bacteriol. 1983 May;154(2):1002-4. doi: 10.1128/jb.154.2.1002-1004.1983.

Abstract

Genes complementing the glucose-negative fructose-negative Saccharomyces cerevisiae triple mutant strain (hxkl hxk2 glk1), which lacks hexokinase PI, hexokinase PII, and glucokinase, were obtained from a pool of yeast DNA in the multicopy plasmid YEp13.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cloning, Molecular*
  • Genes*
  • Genetic Complementation Test
  • Glucokinase / genetics*
  • Hexokinase / genetics*
  • Mutation
  • Plasmids
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*

Substances

  • Hexokinase
  • Glucokinase