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Protein Overview: SUP35

General Information:
Name(s) found: SUP35 / YDR172W [SGD]
Description(s) found:
Found 31 descriptions.
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  • Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 685 amino acids

    Gene Ontology:
    Cellular Component: cytosol [TASLink to PubMed article]
    translation release factor complex [IDALink to PubMed article]
    Biological Process: translational termination [TASLink to PubMed article]
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IMPLink to PubMed article][IPILink to PubMed article]
    Molecular Function: translation release factor activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDSNQGNNQ QNYQQYSQNG NQQQGNNRYQ GYQAYNAQAQ PAGGYYQNYQ GYSGYQQGGY  60
   61 QQYNPDAGYQ QQYNPQGGYQ QYNPQGGYQQ QFNPQGGRGN YKNFNYNNNL QGYQAGFQPQ 120
  121 SQGMSLNDFQ KQQKQAAPKP KKTLKLVSSS GIKLANATKK VGTKPAESDK KEEEKSAETK 180
  181 EPTKEPTKVE EPVKKEEKPV QTEEKTEEKS ELPKVEDLKI SESTHNTNNA NVTSADALIK 240
  241 EQEEEVDDEV VNDMFGGKDH VSLIFMGHVD AGKSTMGGNL LYLTGSVDKR TIEKYEREAK 300
  301 DAGRQGWYLS WVMDTNKEER NDGKTIEVGK AYFETEKRRY TILDAPGHKM YVSEMIGGAS 360
  361 QADVGVLVIS ARKGEYETGF ERGGQTREHA LLAKTQGVNK MVVVVNKMDD PTVNWSKERY 420
  421 DQCVSNVSNF LRAIGYNIKT DVVFMPVSGY SGANLKDHVD PKECPWYTGP TLLEYLDTMN 480
  481 HVDRHINAPF MLPIAAKMKD LGTIVEGKIE SGHIKKGQST LLMPNKTAVE IQNIYNETEN 540
  541 EVDMAMCGEQ VKLRIKGVEE EDISPGFVLT SPKNPIKSVT KFVAQIAIVE LKSIIAAGFS 600
  601 CVMHVHTAIE EVHIVKLLHK LEKGTNRKSK KPPAFAKKGM KVIAVLETEA PVCVETYQDY 660
  661 PQLGRFTLRD QGTTIAIGKI VKIAE

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) SUP35 SUP45
    View Details Krogan NJ, et al. (2006) PLB3 RPS10B SCM3 SUP35 SUP45 TRM12 VPS3
    View Details Gavin AC, et al. (2006) SUP35 SUP45
    View Details Gavin AC, et al. (2002) CLU1 CRN1 ECM29 KAP123 MEC1 MIP6 MIS1 MRPL23 NAB3 NAB6 NRD1 ROM2 RPA135 SEN54 SUI3 SUP35 SUP45 TAO3 USO1 UTP20 VAC14 VPS5 YJL017W
    View Details Qiu et al. (2008) SUP35 SUP45

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run TOR1 No Comments Reinke A, et al. (2004)
    View Run TOR1 No Comments Reinke A, et al. (2004)
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run NOP9 No Comments Hazbun TR, et al. (2003)
    View Run LST8 No Comments Reinke A, et al. (2004)
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run KOG1 No Comments Reinke A, et al. (2004)
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment Keck JM, et al. (2011)
    View Run MLP2 #05 Alpha Factor Prep2-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #07 Alpha Factor Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #09 Alpha Factor Prep4-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data SUP35 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..254] ORFEUS 13.27 No description for 1ldvA was found.
    2 View Details [255..482] PSI-BLAST 1862.218487 Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain
    3 View Details [483..576] PSI-BLAST 1862.218487 Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain
    4 View Details [577..685] PSI-BLAST 1862.218487 Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • translation elongation factor activity
    		•  8.90683166637832 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  4.8378725567094 bayes_pls_golite062009
  • translation regulator activity
    		•  4.79814772079421 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  4.56509805974548 bayes_pls_golite062009
  • structural molecule activity
    		•  3.58406348799895 bayes_pls_golite062009
  • transporter activity
    		•  3.10463051760206 bayes_pls_golite062009
  • transmembrane transporter activity
    		•  2.95886887664984 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  2.95079405147356 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  2.87427509025394 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides
    		•  2.85499564954033 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
    		•  2.85178667290149 bayes_pls_golite062009
  • binding
    		•  2.64537357529343 bayes_pls_golite062009
  • hydrolase activity
    		•  2.55157966585974 bayes_pls_golite062009
  • active transmembrane transporter activity
    		•  2.50494515048649 bayes_pls_golite062009
  • translation release factor activity
    		•  2.49148102494524 bayes_pls_golite062009
  • translation termination factor activity
    		•  2.46464632547226 bayes_pls_golite062009
  • primary active transmembrane transporter activity
    		•  2.39629638016861 bayes_pls_golite062009
  • P-P-bond-hydrolysis-driven transmembrane transporter activity
    		•  2.39600931083427 bayes_pls_golite062009
  • ATPase activity
    		•  2.39535714892224 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
    		•  2.36051733655256 bayes_pls_golite062009
  • ATPase activity, coupled to movement of substances
    		•  2.35906629925754 bayes_pls_golite062009
  • ATPase activity, coupled to transmembrane movement of substances
    		•  2.35679406876731 bayes_pls_golite062009
  • RNA binding
    		•  2.29590582680214 bayes_pls_golite062009
  • ATPase activity, coupled
    		•  2.24901251684901 bayes_pls_golite062009
  • actin filament binding
    		•  2.1683792904715 bayes_pls_golite062009
    		• 2.15810909411803 bayes_pls_golite062009
  • motor activity
    		•  2.11140484670646 bayes_pls_golite062009
  • substrate-specific transporter activity
    		•  1.65609907941094 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.49633029954726 bayes_pls_golite062009
  • actin binding
    		•  1.42666717383185 bayes_pls_golite062009
  • cytoskeletal protein binding
    		•  1.40488517180956 bayes_pls_golite062009
  • microtubule motor activity
    		•  1.35938517416356 bayes_pls_golite062009
  • microfilament motor activity
    		•  1.33993901826495 bayes_pls_golite062009
  • substrate-specific transmembrane transporter activity
    		•  1.3182108176243 bayes_pls_golite062009
  • catalytic activity
    		•  1.00104173493322 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.989226939888218 bayes_pls_golite062009
  • ATP binding
    		•  0.98146619596929 bayes_pls_golite062009
  • DNA binding
    		•  0.967303417236485 bayes_pls_golite062009
  • adenyl ribonucleotide binding
    		•  0.949788783884952 bayes_pls_golite062009
  • adenyl nucleotide binding
    		•  0.931486373329219 bayes_pls_golite062009
  • purine nucleotide binding
    		•  0.929901285538132 bayes_pls_golite062009
  • nucleotide binding
    		•  0.922442819194247 bayes_pls_golite062009
  • purine ribonucleotide binding
    		•  0.920987930180051 bayes_pls_golite062009
  • ribonucleotide binding
    		•  0.920926158369519 bayes_pls_golite062009
  • protein methyltransferase activity
    		•  0.876296121718575 bayes_pls_golite062009
  • mRNA binding
    		•  0.76699944561918 bayes_pls_golite062009
  • ion transmembrane transporter activity
    		•  0.704934361725919 bayes_pls_golite062009
  • kinase activity
    		•  0.691112783105676 bayes_pls_golite062009
  • histone methyltransferase activity
    		•  0.63002958837536 bayes_pls_golite062009
  • RNA helicase activity
    		•  0.6022754485555 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.499834566483656 bayes_pls_golite062009
  • GTPase activity
    		•  0.437665353870877 bayes_pls_golite062009
  • anion transmembrane-transporting ATPase activity
    		•  0.353150780751661 bayes_pls_golite062009
  • guanyl nucleotide binding
    		•  0.296406132096546 bayes_pls_golite062009
  • GTP binding
    		•  0.295705904888768 bayes_pls_golite062009
  • guanyl ribonucleotide binding
    		•  0.29028316917561 bayes_pls_golite062009
  • cation transmembrane transporter activity
    		•  0.282397764628576 bayes_pls_golite062009
  • ribonuclease activity
    		•  0.222424272323909 bayes_pls_golite062009
  • protein binding
    		•  0.170837278876362 bayes_pls_golite062009
  • inorganic anion transmembrane transporter activity
    		•  0.12168417344278 bayes_pls_golite062009
  • helicase activity
    		•  0.0775311459068899 bayes_pls_golite062009
  • general RNA polymerase II transcription factor activity
    		•  0.0716139935283535 bayes_pls_golite062009
  • transcription factor activity
    		•  0.0434163635422169 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  0.0430764033739892 bayes_pls_golite062009
  • protein transporter activity
    		•  0.0397092337273124 bayes_pls_golite062009
  • protein transmembrane transporter activity
    		•  0.0378638259240902 bayes_pls_golite062009
  • protein kinase activity
    		•  0.0364322017534667 bayes_pls_golite062009
  • peptide transporter activity
    		•  0.0135927378126404 bayes_pls_golite062009
  • signal sequence binding
    		•  6.53725193420218E-4 bayes_pls_golite062009
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle