Protein Overview: SEN54

General Information:
Name(s) found:	SEN54 / YPL083C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6325174\|ref\|NP_015242.1\| Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen54p [Saccharomyces cerevisiae] [NCBI NR] gi\|3914974\|sp\|Q02825.1\|SEN54_YEAST RecName: Full=tRNA-splicing endonuclease subunit SEN54; AltName: Full=tRNA-intron endonuclease SEN54; AltName: Full=Splicing endonuclease of 54 kDa [NCBI NR] pir\|\|S61105 hypothetical protein YPL083c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1147611\|gb\|AAB68256.1\| Lpf3p [NCBI NR] tRNA-splicing endonuclease subunit SEN54 OS=Saccharomyces cerevisiae GN=SEN54 PE=1 SV=1 [Swiss-Prot] (Q02825) tRNA-splicing endonuclease subunit SEN54 (tRNA-intron endonuclease SEN54) (Splicing endonuclease of 54 kDa) [Swiss-Prot] SEN54 tRNA splicing endonuclease alpha subunit [mips] Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p [SGD] (Q02825) tRNA-splicing endonuclease subunit SEN54 (EC 3.1.27.9) (tRNA-intron endonuclease) [4932.FASTACCERV.txt] Chr XVI from 398100-396697, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XVI from 396697-398100, reverse complement [yeastorf3.fasta] SEN54, Chr XVI from 396697-398100, reverse complement [yeast_orfs2] SEN54 SGDID:S0006004, Chr XVI from 398100-396697, reverse complement, Verified ORF [yeastflysequest.fasta] SEN54 SGDID:S0006004, Chr XVI from 398102-396699, reverse complement, Verified ORF [10112005cervSGD.fasta] Sen54p [Saccharomyces cerevisiae]gi\|2132181\|pir\|\|S61105 hypothetical protein YPL083c - yeast (Saccharomyces cerevisiae)gi\|1147611\|gb\|AAB68256.1\| Lpf3pgi\|3914974\|sp\|Q02825\|SE54_YEAST tRNA-splicing endonuclease subunit SEN54 (tRNA-intron endonuclease SEN [nrdb_11152004_Chlamydomonas] SEN54 SGDID:S000006004, Chr XVI from 398102-396699, reverse complement, Verified ORF, "Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Lpf3p [nr-271106-contam.fasta] tRNA-splicing endonuclease subunit SEN54 (tRNA-intron endonuclease SEN54) (Splicing endonuclease of 54 kDa) [nr-271106-contam.fasta] Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen54p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] tRNA-splicing endonuclease subunit SEN54 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SEN54 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SEN54 SGDID:S000006004, Chr XVI from 398105-396702, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p" [yeast-030211-contam.fasta] SEN54 SGDID:S000006004, Chr XVI from 398105-396702, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease is composed of Sen2p, Sen15p, Sen34p, and Sen54p" [20141208_orf_trans.fasta] YPL083C SGDID:S000006004, Chr XVI from 398105-396702, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	467 amino acids
Gene Ontology:
Cellular Component:	nuclear inner membrane [TAS] mitochondrion [IDA] tRNA-intron endonuclease complex [TAS] mitochondrial outer membrane [IDA ][IMP][IPI]
Biological Process:	tRNA splicing, via endonucleolytic cleavage and ligation [TAS]
Molecular Function:	tRNA-intron endonuclease activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MQFAGKKTDQ VTTSNPGFEE EEEEEEELQQ DWSQLASLVS KNAALSLPKR GEKDYEPDGT  60
   61 NLQDLLLYNA SKAMFDTISD SIRGTTVKSE VRGYYVPHKH QAVLLKPKGS FMQTMGRADS 120
  121 TGELWLDFHE FVYLAERGTI LPYYRLEAGS NKSSKHETEI LLSMEDLYSL FSSQQEMDQY 180
  181 FVFAHLKRLG FILKPSNQEA AVKTSFFPLK KQRSNLQAIT WRLLSLFKIQ ELSLFSGFFY 240
  241 SKWNFFFRKY TTSPQLYQGL NRLVRSVAVP KNKKELLDAQ SDREFQKVKD IPLTFKVWKP 300
  301 HSNFKKRDPG LPDFQVFVYN KNDDLQHFPT YKELRSMFSS LDYKFEFLSE IEDDDDWETN 360
  361 SYVEDIPRKE YIHKRSAKSQ TEKSESSMKA SFQKKTAQSS TKKKRKAYPP HIQQNRRLKT 420
  421 GYRSFIIAIM DNGLISFVKM SEADFGSESV WYTPNTQKKV DQRWKKH

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		LOS1 SEN15 SEN2 SEN34 SEN54 STP1 STP2 STP3 STP4 TPT1 TRL1
View Details	Gavin AC, et al. (2006)		SEN54 TUF1 VAC14
View Details	Gavin AC, et al. (2002)		CLU1 CRN1 ECM29 KAP123 MEC1 MIP6 MIS1 MRPL23 NAB3 NAB6 NRD1 ROM2 RPA135 SEN54 SUI3 SUP35 SUP45 TAO3 USO1 UTP20 VAC14 VPS5 YJL017W
View Details	Qiu et al. (2008)		SEN15 SEN2 SEN34 SEN54 TRL1 TRM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	SEN54	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..381]	MSA	3.011962	View MSA. No confident structure predictions are available.
2	View Details	[382..467]	deduced	N/A	No confident structure predictions are available.
3	View Details	[207..328]	deduced	N/A	No confident structure predictions are available.
4	View Details	[329..467]	deduced	N/A	No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.93

Protein Overview: SEN54

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: