Protein Overview: CDC5

General Information:
Name(s) found:	CDC5 / YMR001C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|728648\|emb\|CAA88516.1\| Cdc5p [Saccharomyces cerevisiae] [NCBI NR] gi\|6323643\|ref\|NP_013714.1\| Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate; Cdc5p [NCBI NR] gi\|416768\|sp\|P32562.1\|CDC5_YEAST RecName: Full=Cell cycle serine/threonine-protein kinase CDC5/MSD2 [NCBI NR] gi\|172187\|gb\|AAA02576.1\| PKX2 protein kinase [Saccharomyces cerevisiae] [NCBI NR] pir\|\|A48144 protein kinase CDC5 (EC 2.7.1.-) - yeast (Saccharomyces cerevisiae) [NCBI NR] Cell cycle serine/threonine-protein kinase CDC5/MSD2 OS=Saccharomyces cerevisiae GN=CDC5 PE=1 SV=1 [Swiss-Prot] CDC5 Protein kinase, involved in regulation of DNA replication [mips] Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate [SGD] CDC5, Chr XIII from 269019-271136, reverse complement [yeast_orfs2] Chr XIII from 269019-271136, reverse complement [yeastorf3.fasta] Chr XIII from 271136-269019, reverse complement [yeastorf5.fasta] (P32562) Cell cycle protein kinase CDC5/MSD2 (EC 2.7.1.37) [4932.FASTACCERV.txt] CDC5 SGDID:S0004603, Chr XIII from 271136-269019, reverse complement, Verified ORF [yeastflysequest.fasta] Cdc5p [Saccharomyces cerevisiae]gi\|728648\|emb\|CAA88516.1\| Cdc5p [Saccharomyces cerevisiae]gi\|416768\|sp\|P32562\|CDC5_YEAST Cell cycle serine/threonine-protein kinase CDC5/MSD2gi\|1078302\|pir\|\|A48144 protein kinase CDC5 (EC 2.7.1.-) - yeast (Saccharomyces [nrdb_11152004_Chlamydomonas] (P32562) Cell cycle serine/threonine-protein kinase CDC5/MSD2 (EC 2.7.1.37) [cerv4932.FASTAC] CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phos [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate; Cdc5p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Cell cycle serine/threonine-protein kinase CDC5/MSD2 [nr-271106-contam.fasta] Cdc5p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PKX2 protein kinase [nr-271106-contam.fasta] CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Cell cycle serine/threonine-protein kinase CDC5/MSD2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CDC5 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CDC5 SGDID:S000004603, Chr XIII from 271136-269019, Genome Release 64-1-1, reverse complement, Verified ORF, "Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate" [yeast-030211-contam.fasta] CDC5 SGDID:S000004603, Chr XIII from 271136-269019, Genome Release 64-2-1, reverse complement, Verified ORF, "Polo-like kinase; controls targeting and activation of Rho1p at cell division site via Rholp guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate" [20141208_orf_trans.fasta] TR6309\|c0_g1_i1\|g.4457 ORF TR6309\|c0_g1_i1\|g.4457 TR6309\|c0_g1_i1\|m.4457 type:complete len:706 (+) TR6309\|c0_g1_i1:567-2684(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YMR001C SGDID:S000004603, Chr XIII from 271136-269019, Genome Release 64-1-1, reverse complement, Verified ORF, "Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_4086\|g.3085 ORF asmbl_4086\|g.3085 asmbl_4086\|m.3085 type:complete len:706 (+) asmbl_4086:789-2906(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_4091\|g.2615 ORF ID=asmbl_4091\|g.2615 ID=asmbl_4091\|m.2615 type:complete len:706 (+) ID=asmbl_4091:789-2906(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	705 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cellular bud neck [IDA] spindle pole [IDA]
Biological Process:	DNA-dependent DNA replication [IDA][IMP] protein amino acid phosphorylation [IDA ][IMP ]
Molecular Function:	protein kinase activity [IDA ][IMP ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLGPLKAIN DKQLNTRSKL VHTPIKGNTA DLVGKENHFK QTKRLDPNND HHHQPAQKKK  60
   61 REKLSALCKT PPSLIKTRGK DYHRGHFLGE GGFARCFQIK DDSGEIFAAK TVAKASIKSE 120
  121 KTRKKLLSEI QIHKSMSHPN IVQFIDCFED DSNVYILLEI CPNGSLMELL KRRKVLTEPE 180
  181 VRFFTTQICG AIKYMHSRRV IHRDLKLGNI FFDSNYNLKI GDFGLAAVLA NESERKYTIC 240
  241 GTPNYIAPEV LMGKHSGHSF EVDIWSLGVM LYALLIGKPP FQARDVNTIY ERIKCRDFSF 300
  301 PRDKPISDEG KILIRDILSL DPIERPSLTE IMDYVWFRGT FPPSIPSTVM SEAPNFEDIP 360
  361 EEQSLVNFKD CMEKSLLLES MSSDKIQRQK RDYISSIKSS IDKLEEYHQN RPFLPHSLSP 420
  421 GGTKQKYKEV VDIEAQRRLN DLAREARIRR AQQAVLRKEL IATSTNVIKS EISLRILASE 480
  481 CHLTLNGIVE AEAQYKMGGL PKSRLPKIKH PMIVTKWVDY SNKHGFSYQL STEDIGVLFN 540
  541 NGTTVLRLAD AEEFWYISYD DREGWVASHY LLSEKPRELS RHLEVVDFFA KYMKANLSRV 600
  601 STFGREEYHK DDVFLRRYTR YKPFVMFELS DGTFQFNFKD HHKMAISDGG KLVTYISPSH 660
  661 ESTTYPLVEV LKYGEIPGYP ESNFREKLTL IKEGLKQKST IVTVD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		CDC5 HHF1, HHF2 HHO1 ICP55 IRR1 IVY1 KAP95 LOC1 MCD1 MGM101 MUS81 NOP12 NOP13 NUG1 SMC1 SMC3 SPC72 SRP1 TAF14 TMA29 YRA1
View Details	Qiu et al. (2008)		CBC2 CDC14 CDC5 ELM1 PRP28 PRP39

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RIX1	No Comments	Hazbun TR, et al. (2003)
View Run	MLP2	#34 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#16 Mitotic-New search criteria	Keck JM, et al. (2011)
View Run	MLP2	#15 Mitotic 2nd-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	None Entered	#18 Mitotic Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	LIA1	CDC5	1	Unpublished Fields Lab Data
View Screen	CPR6	CDC5	1	Unpublished Fields Lab Data
View Screen	NUP2	CDC5	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CDC5	Huh WK, et al. (2003)
View Data	CDC5	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..72] [125..144] [160..368]	PSI-BLAST	486.0	cAMP-dependent PK, catalytic subunit
2	View Details	[73..124] [145..159] [369..382]	PSI-BLAST	486.0	cAMP-dependent PK, catalytic subunit
3	View Details	[383..455]	PSI-BLAST	17.69897	Calmodulin
4	View Details	[456..519]	PSI-BLAST	17.69897	Calmodulin
5	View Details	[520..602]	ORFEUS	8.38	Sak kinase Polo domain
6	View Details	[603..705]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2