Protein Overview: POL32

General Information:
Name(s) found:	POL32 / YJR043C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322503\|ref\|NP_012577.1\| Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p; Pol32p [Sacc [NCBI NR] gi\|45269727\|gb\|AAS56244.1\| YJR043C [Saccharomyces cerevisiae] [NCBI NR] gi\|1352880\|sp\|P47110.1\|DPOD3_YEAST RecName: Full=DNA polymerase delta subunit 3 [NCBI NR] gi\|1197071\|gb\|AAA88745.1\| ORF; putative [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S57062 hypothetical protein YJR043c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1015697\|emb\|CAA89571.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] DNA polymerase delta subunit 3 OS=Saccharomyces cerevisiae GN=POL32 PE=1 SV=1 [Swiss-Prot] (P47110) DNA polymerase delta subunit 3 [Swiss-Prot] POL32 Polymerase-associated gene, third (55 kDa) subunit of DNA polymerase delta [mips] Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p [SGD] POL32, Chr X from 516153-517205, reverse complement [yeast_orfs2] Chr X from 516153-517205, reverse complement [yeastorf3.fasta] Chr X from 516154-517206, reverse complement [yeastorf4.fasta] Chr X from 517207-516155, reverse complement [yeastorf5.fasta] POL32 SGDID:S0003804, Chr X from 517427-516375, reverse complement, Verified ORF [yeastflysequest.fasta] Pol32p [Saccharomyces cerevisiae]gi\|1015697\|emb\|CAA89571.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|1077882\|pir\|\|S57062 hypothetical protein YJR043c - yeast (Saccharomyces cerevisiae)gi\|45269727\|gb\|AAS56244.1\| YJR043C [Saccharomyces cerevi [nrdb_11152004_Chlamydomonas] POL32 SGDID:S000003804, Chr X from 517427-516375, reverse complement, Verified ORF, "Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; int [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] POL32 SGDID:S000003804, Chr X from 517506-516454, reverse complement, Verified ORF, "Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; int [yeast-contam.fasta] POL32 SGDID:S000003804, Chr X from 517505-516453, reverse complement, Verified ORF, "Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; int [yeast-200209-contam.fasta] ORF; putative [nr-271106-contam.fasta] YJR043C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA polymerase subunit delta 3 [nr-271106-contam.fasta] Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p; Pol32p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] POL32 SGDID:S000003804, Chr X from 517505-516453, reverse complement, Verified ORF, "Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p" [yeast-bovrabprots.fasta] POL32 SGDID:S000003804, Chr X from 517506-516454, reverse complement, Verified ORF, "Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p" [yeast-contam_custom.fasta] [chemostatdb.fasta] DNA polymerase delta subunit 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=POL32 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] POL32 SGDID:S000003804, Chr X from 517513-516461, Genome Release 64-1-1, reverse complement, Verified ORF, "Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p" [yeast-030211-contam.fasta] POL32 SGDID:S000003804, Chr X from 517513-516461, Genome Release 64-2-1, reverse complement, Verified ORF, "Third subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p" [20141208_orf_trans.fasta] YJR043C SGDID:S000003804, Chr X from 517513-516461, Genome Release 64-1-1, reverse complement, Verified ORF, "Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	350 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] delta DNA polymerase complex [TAS]
Biological Process:	mutagenesis [TAS] lagging strand elongation [TAS] base-excision repair [TAS] leading strand elongation [TAS] telomere maintenance [IMP] nucleotide-excision repair [TAS] mismatch repair [NAS ] postreplication repair [TAS]
Molecular Function:	single-stranded DNA specific 3'-5' exodeoxyribonuclease activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDQKASYFIN EKLFTEVKPV LFTDLIHHLK IGPSMAKKLM FDYYKQTTNA KYNCVVICCY  60
   61 KDQTIKIIHD LSNIPQQDSI IDCFIYAFNP MDSFIPYYDI IDQKDCLTIK NSYELKVSES 120
  121 SKIIERTKTL EEKSKPLVRP TARSKTTPEE TTGRKSKSKD MGLRSTALLA KMKKDRDDKE 180
  181 TSRQNELRKR KEENLQKINK QNPEREAQMK ELNNLFVEDD LDTEEVNGGS KPNSPKETDS 240
  241 NDKDKNNDDL EDLLETTAED SLMDVPKIQQ TKPSETEHSK EPKSEEEPSS FIDEDGYIVT 300
  301 KRPATSTPPR KPSPVVKRAL SSSKKQETPS SNKRLKKQGT LESFFKRKAK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		POL31 POL32
View Details	(MIPS) Mewes HW, et al. (2004)		CDC45 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32
View Details	Krogan NJ, et al. (2006)		POL3 POL30 POL31 POL32
View Details	Qiu et al. (2008)		CDC45 CDC9 DPB11 DPB2 DPB3 DPB4 HCS1 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC5 ORC6 POL1 POL2 POL3 POL30 POL31 POL32 PRI1 PRI2 REV3 REV7 RFC1 RFC4 RRM3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HMG1	POL32	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	POL32	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..74]	deduced	N/A	No confident structure predictions are available.
2	View Details	[75..350]	MSA	1.003997	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2