Protein Overview: DPB11

General Information:
Name(s) found:	DPB11 / YJL090C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|929863\|emb\|CAA58477.1\| J0918 [Saccharomyces cerevisiae] [NCBI NR] gi\|6322371\|ref\|NP_012445.1\| Subunit of DNA Polymerase II Epsilon complex; has BRCT domain, required on the prereplicative complex at replication origins for loading DNA polymerases to initiate DNA synthesis, also required for S/M checkpoint control; Dpb11 [NCBI NR] gi\|1352999\|sp\|P47027.1\|DPB11_YEAST RecName: Full=DNA replication regulator DPB11 [NCBI NR] pir\|\|S56017 DPB11 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1008260\|emb\|CAA89383.1\| DPB11 [Saccharomyces cerevisiae] [NCBI NR] DNA replication regulator DPB11 OS=Saccharomyces cerevisiae GN=DPB11 PE=1 SV=1 [Swiss-Prot] (P47027) DNA replication regulator DPB11 [Swiss-Prot] DPB11 Protein involved in DNA replication and S-phase checkpoint [mips] Replication initiation protein that loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1 [SGD] DPB11, Chr X from 262453-264747, reverse complement [yeast_orfs2] Chr X from 262453-264747, reverse complement [yeastorf3.fasta] Chr X from 264747-262453, reverse complement [yeastorf5.fasta] (P47027) Hypothetical 87.2 kDa protein in SRS2-SIP4 intergenic region [4932.FASTACCERV.txt] DPB11 SGDID:S0003626, Chr X from 264967-262673, reverse complement, Verified ORF [yeastflysequest.fasta] Dpb11p [Saccharomyces cerevisiae]gi\|1008260\|emb\|CAA89383.1\| DPB11 [Saccharomyces cerevisiae]gi\|929863\|emb\|CAA58477.1\| J0918 [Saccharomyces cerevisiae]gi\|1077825\|pir\|\|S56017 DPB11 protein - yeast (Saccharomyces cerevisiae)gi\|1352999\|sp\|P47027\|DP11_YEAS [nrdb_11152004_Chlamydomonas] DPB11 SGDID:S000003626, Chr X from 264967-262673, reverse complement, Verified ORF, "Essential BRCT repeat protein, required on the prereplicative complex at replication origins for loading DNA polymerases to initiate DNA synthesis, also required for S/M [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] DPB11 SGDID:S000003626, Chr X from 265046-262752, reverse complement, Verified ORF, "Essential BRCT repeat protein, required on the prereplicative complex at replication origins for loading DNA polymerases to initiate DNA synthesis, also required for S/M [yeast-contam.fasta] DPB11 SGDID:S000003626, Chr X from 265046-262752, reverse complement, Verified ORF, "Subunit of DNA Polymerase II Epsilon complex; has BRCT domain, required on the prereplicative complex at replication origins for loading DNA polymerases to initiate DNA s [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] DPB11 SGDID:S000003626, Chr X from 265045-262751, reverse complement, Verified ORF, "Subunit of DNA Polymerase II Epsilon complex; has BRCT domain, required on the prereplicative complex at replication origins for loading DNA polymerases, also required fo [yeast-200209-contam.fasta] Essential BRCT repeat protein, required on the prereplicative complex at replication origins for loading DNA polymerases to initiate DNA synthesis, also required for S/M checkpoint control; Dpb11p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] J0918 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA replication regulator DPB11 [nr-271106-contam.fasta] DPB11 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DPB11 SGDID:S000003626, Chr X from 265045-262751, reverse complement, Verified ORF, "Replication initiation protein that loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the check [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] DPB11 SGDID:S000003626, Chr X from 265045-262751, reverse complement, Verified ORF, "Subunit of DNA Polymerase II Epsilon complex; has BRCT domain, required on the prereplicative complex at replication origins for loading DNA polymerases, also required for S/M checkpoint control; activates Mec1p kinase activity" [yeast-bovrabprots.fasta] DPB11 SGDID:S000003626, Chr X from 265046-262752, reverse complement, Verified ORF, "Essential BRCT repeat protein, required on the prereplicative complex at replication origins for loading DNA polymerases to initiate DNA synthesis, also required for S/M checkpoint control" [yeast-contam_custom.fasta] [chemostatdb.fasta] DPB11 SGDID:S000003626, Chr X from 265045-262751, reverse complement, Verified ORF, "Replication initiation protein that loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1" [SGDyeast.20101221.fasta] DNA replication regulator DPB11 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DPB11 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DPB11 SGDID:S000003626, Chr X from 265051-262757, Genome Release 64-1-1, reverse complement, Verified ORF, "Replication initiation protein that loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1" [yeast-030211-contam.fasta] DPB11 SGDID:S000003626, Chr X from 265051-262757, Genome Release 64-2-1, reverse complement, Verified ORF, "DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress" [20141208_orf_trans.fasta] YJL090C SGDID:S000003626, Chr X from 265051-262757, Genome Release 64-1-1, reverse complement, Verified ORF, "Replication initiation protein that loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	764 amino acids
Gene Ontology:
Cellular Component:	replication fork [IDA][TAS]
Biological Process:	DNA replication initiation [TAS] DNA replication checkpoint [IMP] lagging strand elongation [TAS] recombinational repair [IDA][IGI] leading strand elongation [TAS] nucleotide-excision repair [TAS] mismatch repair [NAS]
Molecular Function:	protein binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKPFQGITFC PTAINNEILA KKISKKIIKL GGIFSKDLTR QVNVLVVGST TNTNKFKFAV  60
   61 KHRFDIIFID IQAIDDIYQL WLSGENILPD SNTATMTGST YEMLKILYRR FSFKYLHNFN 120
  121 IFIGRITDTN ITSIDSLVRS IKKLGCSSYN YQNFVIKDTS SHNDDDDQGQ NGQISIFVTD 180
  181 TLLGARVNAA IEQNIPIVHF KWILDCQKRS ALLPYDPYYL LPNIKDLPYD SIGSNSCDCW 240
  241 DKINTTFPTN IDAQSSLQRQ QSSSTLTPSL PKTSSLLNKF KPKGEKIWDK AMSLQQHSKT 300
  301 NFSVLGQSPL SINNKQEDLS DNSTLIFKNC AFIIHHIFPG NHRSILTKIV VQNGGKIETS 360
  361 YLSGIYDHSY YIIPSNKALD SFNDLPEIID DNDGIVTEFF IERCLYYQKL LHPIDLWSKP 420
  421 FLSTIEFQVS SSSKLLHHEF SSSPFLNVTI TGFSGVELLH LTKVLNLLKP MGINYVEYLN 480
  481 KSTDILLINL AALPSIPKTH PLWSNEFSDL FTQFCINNNN DDPGDNNRKD FQNNSILRNS 540
  541 MKRKIEYIKK FHSIPVVTPA FIFKLLSAAS GENNEIFLNN IKWCIICPRG HKDDFKCKIK 600
  601 KPYYTSISSE KKYQNNDPKI DKTILLKRNN SSLSEHSMKD TKNELLQKIR ETDSGRKKRS 660
  661 VSSSIMDVSS ERQMPDTKRI KLESLPKNFV PKQIKRTTSW GTIMSENVPT EQPTAISNPE 720
  721 EIPRTEEVSH TQVTYGSIQD KKRTASLEKP MRRQTRNQTK ELDS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		CDC45 CDC6 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32 SLD3
View Details	Ho Y, et al. (2002)		DPB11 NMD3 SRP1 TIF4631
View Details	Qiu et al. (2008)		CDC45 DPB11 DPB2 DPB3 DPB4 ELG1 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 MRC1 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL30 POL31 POL32 RFC1 RFC2 RFC4 SLD3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	PAN1	No Comments	Toshima J, et al (2006)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ELA1	DPB11	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	DPB11	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..124]	ORFEUS	11.3	Breast cancer associated protein, BRCA1
2	View Details	[125..263]	ORFEUS	11.3	Breast cancer associated protein, BRCA1
3	View Details	[264..320]	deduced	N/A	No confident structure predictions are available.
4	View Details	[321..429]	Pfam	8.522879	BRCA1 C Terminus (BRCT) domain No confident structure predictions are available.
5	View Details	[430..764]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.92

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2