Protein Overview: MSH1

General Information:
Name(s) found:	MSH1 / YHR120W [SGD]
Description(s) found: Found 22 descriptions. SHOW ALL	DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis [SGD]
Organism:	Saccharomyces cerevisiae
Length:	959 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ]
Biological Process:	DNA repair [IMP] [NOT]reciprocal meiotic recombination [IMP] mismatch repair [IMP] mitochondrion organization [IMP ] mitochondrial genome maintenance [IMP]
Molecular Function:	DNA-dependent ATPase activity [IDA] dinucleotide insertion or deletion binding [IDA] guanine/thymine mispair binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKHFFRLPTA FRPISRVSLR YSSTDTAQPK ISKLKISFNK ISESNSEKKD NLGSIDTRNC  60
   61 LSTQQDDKLS STEPSKASLP PSLQYVRDLM DLYKDHVVLT QMGSFYELYF EQAIRYAPEL 120
  121 NISLTNRAYS HGKVPFAGFP VHQLSRHLKM LVNNCGYSVT IAEQFKKKDV ADNEANKFYR 180
  181 RVTRIVTPGT FIDEAFENLR ENTYLLNIEF PENCMSQVAD TSLKVGICWC DVSTGEIFVQ 240
  241 QVYLRDLVSA ITRIQPKEIL LDERLLEFHI ESGTWYPELV ELKKFFIKYQ KMPSQHRTIE 300
  301 SFYGLFNLGG KEATERQLKI QFQTFTQKEL AALRNTLIYV SNHLPDFSIN FQIPQRQLAT 360
  361 AIMQIDSRTS TALELHSTVR DNNKKGSLLS SIRRTVTPSG TRLLSQWLSG PSLDLKEIKK 420
  421 RQKIVAFFKD NRDITETLRT MLKKVNDLSR ILQKFSFGRG EALELIQMAR SLEVSREIRK 480
  481 YLLNNTSLMK ATLKSQITQL TESLNFEKNL IDDILKFLNE EELAKSQDAK QNADVTRMLD 540
  541 IDVKDKKESN KDEIFELRDF IVNPSFNTKL RKLHDTYQGV WQKKTEYNAL LKGFFVGDLG 600
  601 AKTFTLKERQ NGEYALHVTG TASSLKKIDE LISKSTEYHG SCFHILQKSS QTRWLSHKIW 660
  661 TDLGHELELL NLKIRNEEAN IIDLFKRKFI DRSNEVRQVA TTLGYLDTLS SFAVLANERN 720
  721 LVCPKVDESN KLEVVNGRHL MVEEGLSARS LETFTANNCE LAKDNLWVIT GPNMGGKSTF 780
  781 LRQNAIIVIL AQIGCFVPCS KARVGIVDKL FSRVGSADDL YNEMSTFMVE MIETSFILQG 840
  841 ATERSLAILD EIGRGTSGKE GISIAYATLK YLLENNQCRT LFATHFGQEL KQIIDNKCSK 900
  901 GMSEKVKFYQ SGITDLGGNN FCYNHKLKPG ICTKSDAIRV AELAGFPMEA LKEAREILG

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		MAS1 MAS2 MSH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	MSH1	YOR302W	2	Unpublished Fields Lab Data
View Screen	MSH1	HSP26	1	Unpublished Fields Lab Data
View Screen	MSH1	TCB3	1	Unpublished Fields Lab Data
View Screen	MSH1	IST2	1	Unpublished Fields Lab Data
View Screen	MSH1	MVP1	1	Unpublished Fields Lab Data
View Screen	MSH1	UMP1	1	Unpublished Fields Lab Data
View Screen	MSH1	RTT106	1	Unpublished Fields Lab Data
View Screen	MSH1	RMD5	1	Unpublished Fields Lab Data
View Screen	MSH1	SRP101	1	Unpublished Fields Lab Data
View Screen	UBC7	MSH1	1	Unpublished Fields Lab Data
View Screen	MSH1	YER187W	1	Unpublished Fields Lab Data
View Screen	MSH1	HAP2	1	Unpublished Fields Lab Data
View Screen	MSH1	YAP5	1	Unpublished Fields Lab Data
View Screen	MSH1	SMC3	1	Unpublished Fields Lab Data
View Screen	MSH1	YJL113W	1	Unpublished Fields Lab Data
View Screen	MSH1	SSC1	1	Unpublished Fields Lab Data
View Screen	MSH1	HIR3	1	Unpublished Fields Lab Data
View Screen	MSH1	VBA5	1	Unpublished Fields Lab Data
View Screen	MSH1	SSA2	1	Unpublished Fields Lab Data
View Screen	MSH1	ENT4	1	Unpublished Fields Lab Data
View Screen	HMT1	MSH1	1	Unpublished Fields Lab Data
View Screen	MSH1	JIP3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MSH1	Huh WK, et al. (2003)
View Data	MSH1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..68]	deduced	N/A	No confident structure predictions are available.
2	View Details	[69..185]	PSI-BLAST	1421.54902	DNA repair protein MutS, domain III; DNA repair protein MutS, the C-terminal domain; DNA repair protein MutS, domain II; DNA repair protein MutS, domain I
3	View Details	[186..360]	PSI-BLAST	1421.54902	DNA repair protein MutS, domain III; DNA repair protein MutS, the C-terminal domain; DNA repair protein MutS, domain II; DNA repair protein MutS, domain I
4	View Details	[361..386] [717..959]	PSI-BLAST	1421.54902	DNA repair protein MutS, domain III; DNA repair protein MutS, the C-terminal domain; DNA repair protein MutS, domain II; DNA repair protein MutS, domain I
5	View Details	[387..577] [669..716]	PSI-BLAST	1421.54902	DNA repair protein MutS, domain III; DNA repair protein MutS, the C-terminal domain; DNA repair protein MutS, domain II; DNA repair protein MutS, domain I
6	View Details	[578..668]	PSI-BLAST	1421.54902	DNA repair protein MutS, domain III; DNA repair protein MutS, the C-terminal domain; DNA repair protein MutS, domain II; DNA repair protein MutS, domain I

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
DNA-dependent ATPase activity	2.72462789867829	bayes_pls_golite062009
mismatched DNA binding	2.51625633651862	bayes_pls_golite062009
binding	2.4974940028864	bayes_pls_golite062009
DNA helicase activity	2.10134737488849	bayes_pls_golite062009
double-strand/single-strand DNA junction binding	2.02697209084624	bayes_pls_golite062009
	1.85506769032946	bayes_pls_golite062009
nucleoside-triphosphatase activity	1.84050240798436	bayes_pls_golite062009
pyrophosphatase activity	1.81138802583782	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	1.78927528875848	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	1.78903889318615	bayes_pls_golite062009
ATP-dependent DNA helicase activity	1.71882026185027	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
guanine/thymine mispair binding	1.69026972999845	bayes_pls_golite062009
DNA insertion or deletion binding	1.68890613599528	bayes_pls_golite062009
microtubule motor activity	1.6537049553461	bayes_pls_golite062009
RNA helicase activity	1.63960194106079	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.61735400124552	bayes_pls_golite062009
nucleic acid binding	1.60768715809391	bayes_pls_golite062009
RNA-dependent ATPase activity	1.60404584546501	bayes_pls_golite062009
oxidized purine DNA binding	1.30673778631233	bayes_pls_golite062009
oxidized DNA binding	1.30673778631233	bayes_pls_golite062009
single guanine insertion binding	1.30673778631233	bayes_pls_golite062009
hydrolase activity	1.29419296935491	bayes_pls_golite062009
structure-specific DNA binding	1.2539059700735	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATPase activity	1.18431260772292	bayes_pls_golite062009
motor activity	1.14667913749579	bayes_pls_golite062009
double-stranded DNA binding	1.117585097627	bayes_pls_golite062009
DNA binding	1.11699929651302	bayes_pls_golite062009
protein-DNA loading ATPase activity	1.08464552192299	bayes_pls_golite062009
DNA-directed DNA polymerase activity	1.02269224635866	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
transcription regulator activity	0.989226939888218	bayes_pls_golite062009
DNA polymerase activity	0.987648886158896	bayes_pls_golite062009
DNA clamp loader activity	0.91282941965371	bayes_pls_golite062009
dinucleotide repeat insertion binding	0.645326857719269	bayes_pls_golite062009
single base insertion or deletion binding	0.629563765934841	bayes_pls_golite062009
single-stranded DNA binding	0.568457189190858	bayes_pls_golite062009
cytoskeletal protein binding	0.500462538667228	bayes_pls_golite062009
protein binding	0.193768631623192	bayes_pls_golite062009
nucleotide binding	0.114848452385726	bayes_pls_golite062009
purine nucleotide binding	0.10713474095071	bayes_pls_golite062009
purine ribonucleotide binding	0.0916519414118148	bayes_pls_golite062009
ribonucleotide binding	0.0915913646217636	bayes_pls_golite062009
transcription factor activity	0.0434163635422169	bayes_pls_golite062009
actin binding	0.00482938540552702	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.95

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2