Protein Overview: MDJ1

General Information:
Name(s) found:	MDJ1 / YFL016C [SGD]
Description(s) found: Found 29 descriptions. SHOW ALL	Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones [SGD]
Organism:	Saccharomyces cerevisiae
Length:	511 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA] mitochondrial inner membrane [IDA]
Biological Process:	proteolysis [IMP] protein folding [IDA]
Molecular Function:	chaperone binding [IMP] unfolded protein binding [IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAFQQGVLSR CSGVFRHHVG HSRHINNILY RHAIAFASIA PRIPKSSFHT SAIRNNEAFK  60
   61 DPYDTLGLKK SATGAEIKKA YYKLAKKYHP DINKEPDAEK KFHDLQNAYE ILSDETKRQQ 120
  121 YDQFGPAAFG GGGAAGGAGG GSGSPFGSQF HDFSGFTSAG GSPFGGINFE DLFGAAFGGG 180
  181 GRGSGGASRS SSMFRQYRGD PIEIVHKVSF KDAVFGSKNV QLRFSALDPC STCSGTGMKP 240
  241 NTHKVSCSTC HGTGTTVHIR GGFQMMSTCP TCNGEGTMKR PQDNCTKCHG EGVQVNRAKT 300
  301 ITVDLPHGLQ DGDVVRIPGQ GSYPDIAVEA DLKDSVKLSR GDILVRIRVD KDPNFSIKNK 360
  361 YDIWYDKEIP ITTAALGGTV TIPTVEGQKI RIKVAPGTQY NQVISIPNMG VPKTSTIRGD 420
  421 MKVQYKIVVK KPQSLAEKCL WEALADVTND DMAKKTMQPG TAAGTAINEE ILKKQKQEEE 480
  481 KHAKKDDDNT LKRLENFITN TFRKIKGDKK N

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		MDJ1 RPL11B
View Details	Krogan NJ, et al. (2006)		MDJ1 SCC2 SCC4
View Details	Ho Y, et al. (2002)		ACC1 CCT6 CDC33 CPR6 DMC1 DPM1 FMP10 MDJ1 MTC5 NOP14 NPR2 POX1 RNQ1 RVB2 SEH1 THI21 TRP3 YHR033W
View Details	Qiu et al. (2008)		HSP78 MDJ1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	MIA40	No Comments	Hazbun TR, et al. (2003)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PRE1	MDJ1	1	Unpublished Fields Lab Data
View Screen	RAD6	MDJ1	1	Unpublished Fields Lab Data
View Screen	GTS1	MDJ1	1	Unpublished Fields Lab Data
View Screen	UBC7	MDJ1	1	Unpublished Fields Lab Data
View Screen	CTF13	MDJ1	1	Unpublished Fields Lab Data
View Screen	HRB1	MDJ1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MDJ1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..180]	PSI-BLAST	169.0	DnaJ chaperone, N-terminal (J) domain
2	View Details	[181..296]	PSI-BLAST	142.64593	Cysteine-rich domain of the chaperone protein DnaJ.
3	View Details	[297..511]	PSI-BLAST	22.30103	Heat shock protein 40 Sis1

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
unfolded protein binding	3.04403163860617	bayes_pls_golite062009
chaperone binding	2.86745334638583	bayes_pls_golite062009
protein binding	2.65982681685488	bayes_pls_golite062009
binding	2.63138872417193	bayes_pls_golite062009
transcription regulator activity	2.31739509739943	bayes_pls_golite062009
heat shock protein binding	2.17994194200939	bayes_pls_golite062009
DNA binding	1.88444505115207	bayes_pls_golite062009
nucleic acid binding	1.73387701301727	bayes_pls_golite062009
transporter activity	1.29863098651348	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	0.927337691912581	bayes_pls_golite062009
substrate-specific transporter activity	0.812144232432417	bayes_pls_golite062009
enzyme regulator activity	0.675735261884317	bayes_pls_golite062009
protein transporter activity	0.599800483479766	bayes_pls_golite062009
Hsp70 protein binding	0.541755903922701	bayes_pls_golite062009
GTPase regulator activity	0.130185025729857	bayes_pls_golite062009
catalytic activity	0.106988907594358	bayes_pls_golite062009

Term	Confidence	Notes
binding	2.7298921704023	bayes_pls_golite062009
protein binding	2.6297172985864	bayes_pls_golite062009
unfolded protein binding	2.04158808514315	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	0.594882036388263	bayes_pls_golite062009
receptor binding	0.395342079633271	bayes_pls_golite062009
enzyme regulator activity	0.301226589648736	bayes_pls_golite062009
protein disulfide isomerase activity	0.290861970307477	bayes_pls_golite062009
intramolecular oxidoreductase activity, transposing S-S bonds	0.290861970307477	bayes_pls_golite062009
catalytic activity	0.276917867171685	bayes_pls_golite062009
intramolecular oxidoreductase activity, interconverting keto- and enol-groups	0.0964866497718595	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.68

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2