Protein Overview: GRX4

General Information:
Name(s) found:	GRX4 / YER174C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321022\|ref\|NP_011101.1\| Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; Grx4p [Saccharomyces cerevisiae] [NCBI NR] gi\|603415\|gb\|AAB64701.1\| Yer174cp [Saccharomyces cerevisiae] [NCBI NR] gi\|418432\|sp\|P32642.1\|GLRX4_YEAST RecName: Full=Monothiol glutaredoxin-4 [NCBI NR] pir\|\|S30860 hypothetical protein YER174c - yeast (Saccharomyces cerevisiae) [NCBI NR] (P32642) Monothiol glutaredoxin 4 [Swiss-Prot] Monothiol glutaredoxin-4 OS=Saccharomyces cerevisiae GN=GRX4 PE=1 SV=1 [Swiss-Prot] GRX4 Member of the subfamily of yeast glutaredoxins (Grx3, Grx4, and Grx5) [mips] Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage [SGD] GRX4, Chr V from 538429-539163, reverse complement [yeast_orfs2] Chr V from 538429-539163, reverse complement [yeastorf3.fasta] Chr V from 539163-538429, reverse complement [yeastorf5.fasta] (P32642) Hypothetical 27.5 kDa protein in RAD24-BMH1 intergenic region [4932.FASTACCERV.txt] GRX4 SGDID:S0000976, Chr V from 539163-538429, reverse complement, Verified ORF [yeastflysequest.fasta] Grx4p [Saccharomyces cerevisiae]gi\|320744\|pir\|\|S30860 hypothetical protein YER174c - yeast (Saccharomyces cerevisiae)gi\|603415\|gb\|AAB64701.1\| Yer174cp [Saccharomyces cerevisiae]gi\|418432\|sp\|P32642\|GRX4_YEAST Monothiol glutaredoxin 4 [nrdb_11152004_Chlamydomonas] GRX4 SGDID:S000000976, Chr V from 539163-538429, reverse complement, Verified ORF, "Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells fr [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; Grx4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Yer174cp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Monothiol glutaredoxin-4 [nr-271106-contam.fasta] GRX4 SGDID:S000000976, Chr V from 539163-538429, reverse complement, Verified ORF, "Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Monothiol glutaredoxin-4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GRX4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GRX4 SGDID:S000000976, Chr V from 539168-538434, Genome Release 64-1-1, reverse complement, Verified ORF, "Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance" [yeast-030211-contam.fasta] GRX4 SGDID:S000000976, Chr V from 539168-538434, Genome Release 64-2-1, reverse complement, Verified ORF, "Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; with Grx3p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; mutant has increased aneuploidy tolerance; transcription regulated by Yap5p; GRX4 has a paralog, GRX3, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YER174C SGDID:S000000976, Chr V from 539168-538434, Genome Release 64-1-1, reverse complement, Verified ORF, "Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	244 amino acids
Gene Ontology:
Cellular Component:	nucleus [IGI][IPI] intracellular [TAS]
Biological Process:	cellular iron ion homeostasis [IGI][IPI] response to oxidative stress [IGI][IMP ]
Molecular Function:	thiol-disulfide exchange intermediate activity [IGI][IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTVVEIKSQD QFTQLTTTNA ANKLIVLYFK AQWADPCKTM SQVLEAVSEK VRQEDVRFLS  60
   61 IDADEHPEIS DLFEIAAVPY FVFIQNGTIV KEISAADPKE FVKSLEILSN ASASLANNAK 120
  121 GPKSTSDEES SGSSDDEEDE TEEEINARLV KLVQAAPVML FMKGSPSEPK CGFSRQLVGI 180
  181 LREHQIRFGF FDILRDENVR QSLKKFSDWP TFPQLYINGE FQGGLDIIKE SIEEDPEYFQ 240
  241 HALQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BUD32 CGI121 GRX3 GRX4 KAE1
View Details	Krogan NJ, et al. (2006)		BUD32 GRX4
View Details	Ho Y, et al. (2002)		AAC3 BUD32 CAR2 CGI121 CPR6 CRM1 DIA4 FRA1 FRA2 GRX3 GRX4 HEF3 IDP2 IMD2 IMD4 KAE1 NPA3 PHO81 POR1 RNH202 RPN1 RPN5 RPN6 RPT1 RPT3 SEC18 SEC23 SGO1 TMA29 URA7 YHR033W

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	GRX4	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..137]	PSI-BLAST	97.29073	Thioredoxin
2	View Details	[138..244]	PSI-BLAST	4.69897	Glutaredoxin (Grx, thioltransferase)

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: GRX4

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: