Protein Overview: XRS2

General Information:
Name(s) found:	XRS2 / YDR369C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|849190\|gb\|AAB64805.1\| Xrs2p: DNA repair protein [Saccharomyces cerevisiae] [NCBI NR] gi\|6320577\|ref\|NP_010657.1\| Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling; Xrs2p [Saccharomyces cerevisiae] [NCBI NR] gi\|465488\|sp\|P33301.1\|XRS2_YEAST RecName: Full=DNA repair protein XRS2 [NCBI NR] gi\|349198\|gb\|AAA35220.1\| XRS2 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S61164 repair/recombination protein XRS2 - yeast (Saccharomyces cerevisiae) [NCBI NR] DNA repair protein XRS2 OS=Saccharomyces cerevisiae GN=XRS2 PE=1 SV=1 [Swiss-Prot] XRS2 DNA repair protein [mips] Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling [SGD] XRS2, Chr IV from 1215005-1217569, reverse complement [yeast_orfs2] Chr IV from 1215005-1217569, reverse complement [yeastorf3.fasta] Chr IV from 1215004-1217568, reverse complement [yeastorf4.fasta] Chr IV from 1217568-1215004, reverse complement [yeastorf5.fasta] (P33301) DNA repair protein XRS2 [4932.FASTACCERV.txt] XRS2 SGDID:S0002777, Chr IV from 1217568-1215004, reverse complement, Verified ORF [yeastflysequest.fasta] XRS2 SGDID:S0002777, Chr IV from 1217570-1215006, reverse complement, Verified ORF [10112005cervSGD.fasta] Xrs2p [Saccharomyces cerevisiae]gi\|849190\|gb\|AAB64805.1\| Xrs2p: DNA repair protein [Saccharomyces cerevisiae]gi\|465488\|sp\|P33301\|XRS2_YEAST DNA repair protein XRS2gi\|2133087\|pir\|\|S61164 repair/recombination protein XRS2 - yeast (Saccharomyces cerevisia [nrdb_11152004_Chlamydomonas] XRS2 SGDID:S000002777, Chr IV from 1217570-1215006, reverse complement, Verified ORF, "Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] XRS2 SGDID:S000002777, Chr IV from 1217572-1215008, reverse complement, Verified ORF, "Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint [yeast-contam.fasta] XRS2 SGDID:S000002777, Chr IV from 1217573-1215009, reverse complement, Verified ORF, "Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] DNA repair protein XRS2 [nr-271106-contam.fasta] XRS2 [nr-271106-contam.fasta] Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling; Xrs2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Xrs2p: DNA repair protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] XRS2 SGDID:S000002777, Chr IV from 1217573-1215009, reverse complement, Verified ORF, "Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling" [yeast-bovrabprots.fasta] XRS2 SGDID:S000002777, Chr IV from 1217572-1215008, reverse complement, Verified ORF, "Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling" [yeast-contam_custom.fasta] [chemostatdb.fasta] DNA repair protein XRS2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=XRS2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] XRS2 SGDID:S000002777, Chr IV from 1217580-1215016, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling" [yeast-030211-contam.fasta] XRS2 SGDID:S000002777, Chr IV from 1217580-1215016, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling" [20141208_orf_trans.fasta] YDR369C SGDID:S000002777, Chr IV from 1217580-1215016, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	854 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] Mre11 complex [IPI ]
Biological Process:	double-strand break repair via break-induced replication [TAS] telomere maintenance [IMP ] double-strand break repair via nonhomologous end joining [IMP] sporulation resulting in formation of a cellular spore [IMP] meiotic DNA double-strand break formation [IMP]
Molecular Function:	DNA binding [IDA] protein binding [IPI] telomeric DNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MWVVRYQNTL EDGSISFISC CLQAFKTYSI GRSSKNPLII KNDKSISRQH ITFKWEINNS  60
   61 SDLKHSSLCL VNKGKLTSLN KKFMKVGETF TINASDVLKS TIIELGTTPI RIEFEWINEV 120
  121 WNIPPHLTQF RTMLSEYGIS TEISINDIPA NLMISDYPKS EDNSIRELYA LVSTIPMKKS 180
  181 RFLMELCNTL LPTSKTNLKF DEMWNDMISN PEYNVFDFDP NILLSKFMRL NNIRVLTTIK 240
  241 SEPRLSSLLR TFNINLFAFD NIDSLYKYVD SLEASTEYLI LTTTDKKENG KILCTIKTML 300
  301 TSIIDGTLSA VINMKGASSR TLDNGKFDQI SEGMSTILKT SRAPEVEASP VVSKKRKLNR 360
  361 RRVLPLDSLD FFAGGLSTKT LSENRSLTDA KRLNCGAESK TVISSPNIAE ADEKHAPFLQ 420
  421 NALKPTEDIG KKSGHSSPGA IIVSSPNLGT VNTSEDSLDK SLQSHKLPQP SLPEVAGIGS 480
  481 QTISSNSADY ETAAVNSMDD AEVTKNFRVN HHQNIEQPSK NIRKLSNYSR EISSPLQENC 540
  541 KSPVKELSIK EKSGTPHAFV EAIQETKNRE VKRVKSTIVE LKDEELSEEA INQLKNLAIV 600
  601 EPSNNLLRKS FDSEGNKTSR TTEKWENSLM EPEWHKRKNF KTFVKVRPKS KAHKEEGKNN 660
  661 TQSSDFIRNA AFLITRNYVP LKKYSKKDTT TKWGTEENED MFALTEMERF GSNTFMSDNI 720
  721 NSNTIQKRSQ ALNRFTNEDS SNEGEEDSFS FSRCSGTAAS VQPLKNKIFI TDEDDLGDID 780
  781 DKSDRLNHRE NNRNLFVVKE MNLRPNLSEE CSKQSRHSRS ATSRSRGSFG ASNNGDGDDD 840
  841 DDDGPKFTFK RRKG

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		MRE11 XRS2
View Details	(MIPS) Mewes HW, et al. (2004)		MRE11 RAD50 XRS2
View Details	Krogan NJ, et al. (2006)		DMC1 MRE11 RAD50 XRS2 YBL044W
View Details	Ho Y, et al. (2002)		AHP1 CDC16 DUN1 ERG20 GPH1 MAM33 MKT1 MRE11 PST2 RAD50 REX2 RPT3 SEC27 TFP1 VMA8 XRS2 YBR063C
View Details	Qiu et al. (2008)		MRE11 RAD50 XRS2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC14	XRS2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	XRS2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..157]	Pfam	9.468521	FHA domain No confident structure predictions are available.
2	View Details	[158..487]	deduced	N/A	No confident structure predictions are available.
3	View Details	[488..854]	MSA	1.002821	View MSA. No confident structure predictions are available.
4	View Details	[498..716]	deduced	N/A	No confident structure predictions are available.
5	View Details	[717..854]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	3.29282091000252	bayes_pls_golite062009
protein binding	2.10386508653975	bayes_pls_golite062009
histone binding	1.9804501088198	bayes_pls_golite062009
structure-specific DNA binding	1.80379362156554	bayes_pls_golite062009
DNA binding	1.75711393068958	bayes_pls_golite062009
nucleic acid binding	1.68733623580238	bayes_pls_golite062009
double-stranded DNA binding	1.64875926701369	bayes_pls_golite062009
transcription regulator activity	1.58213576302578	bayes_pls_golite062009
ubiquitin-protein ligase activity	1.32517364912417	bayes_pls_golite062009
chromatin binding	1.24086020252344	bayes_pls_golite062009
small conjugating protein ligase activity	1.23750705623221	bayes_pls_golite062009
transforming growth factor beta receptor, cytoplasmic mediator activity	1.09317005113308	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase signaling protein activity	0.896922420561378	bayes_pls_golite062009
SMAD binding	0.724800831671259	bayes_pls_golite062009
hydrolase activity	0.721388646103233	bayes_pls_golite062009
sequence-specific DNA binding	0.670370530131427	bayes_pls_golite062009
transcription factor activity	0.539168752590071	bayes_pls_golite062009
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity	0.537420543086991	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.520434329676854	bayes_pls_golite062009
transcription activator activity	0.444903517674531	bayes_pls_golite062009
ubiquitin binding	0.306130468133543	bayes_pls_golite062009
acid-amino acid ligase activity	0.295271541480997	bayes_pls_golite062009
small conjugating protein binding	0.217876362477623	bayes_pls_golite062009
damaged DNA binding	0.182219412947843	bayes_pls_golite062009
receptor signaling protein activity	0.00902288416721542	bayes_pls_golite062009
promoter binding	0.00673732596278498	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2