Protein Overview: HSP82

General Information:
Name(s) found:	HSP82 / YPL240C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|HHBY90 heat shock protein 90 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6325016\|ref\|NP_015084.1\| Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, C [NCBI NR] gi\|171725\|gb\|AAA02743.1\| hsp82 protein [NCBI NR] gi\|1370495\|emb\|CAA97961.1\| HSP82 [Saccharomyces cerevisiae] [NCBI NR] gi\|123677\|sp\|P02829.1\|HSP82_YEAST RecName: Full=ATP-dependent molecular chaperone HSP82; AltName: Full=Heat shock protein Hsp90 heat-inducible isoform; AltName: Full=82 kDa heat shock protein [NCBI NR] gi\|1061249\|emb\|CAA91604.1\| HSP90/HSP82? [Saccharomyces cerevisiae] [NCBI NR] ATP-dependent molecular chaperone HSP82 OS=Saccharomyces cerevisiae GN=HSP82 PE=1 SV=1 [Swiss-Prot] (P02829) Heat shock protein HSP82 [Swiss-Prot] HSP82 Heat shock protein [mips] Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, Cpr7p, Sti1p [SGD] Chr XVI from 98625-96496, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XVI from 96496-98625, reverse complement [yeastorf3.fasta] HSP82, Chr XVI from 96496-98625, reverse complement [yeast_orfs2] HSP82 SGDID:S0006161, Chr XVI from 98625-96496, reverse complement, Verified ORF [yeastflysequest.fasta] Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p [Saccharomyces [nrdb_11152004_Chlamydomonas] HSP82 SGDID:S000006161, Chr XVI from 98625-96496, reverse complement, Verified ORF, "Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] HSP82 SGDID:S000006161, Chr XVI from 98625-96496, reverse complement, Verified ORF, "Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA bindin [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p; Hsp82p [Sacch [nr-271106-contam.fasta] hsp82 protein [nr-271106-contam.fasta] ATP-dependent molecular chaperone HSP82 (Heat shock protein Hsp90 heat-inducible isoform) (82 kDa heat shock protein) [nr-271106-contam.fasta] HSP90/HSP82? [Saccharomyces cerevisiae] [nr-271106-contam.fasta] HSP82 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] HSP82 SGDID:S000006161, Chr XVI from 98625-96496, reverse complement, Verified ORF, "Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, Cpr7p, Sti1p" [yeast-bovrabprots.fasta] HSP82 SGDID:S000006161, Chr XVI from 98625-96496, reverse complement, Verified ORF, "Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p" [yeast-contam_custom.fasta] [chemostatdb.fasta] ATP-dependent molecular chaperone HSP82 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP82 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] HSP82 SGDID:S000006161, Chr XVI from 98625-96496, Genome Release 64-1-1, reverse complement, Verified ORF, "Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, Cpr7p, Sti1p" [yeast-030211-contam.fasta] HSP82 SGDID:S000006161, Chr XVI from 98625-96496, Genome Release 64-2-1, reverse complement, Verified ORF, "Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YPL240C SGDID:S000006161, Chr XVI from 98625-96496, Genome Release 64-1-1, reverse complement, Verified ORF, "Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, Cpr7p, Sti1p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	709 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA ]
Biological Process:	response to osmotic stress [IMP] protein refolding [IMP] proteasome assembly [IDA][IGI][IMP] 'de novo' protein folding [IDA][IMP] response to stress [IMP]
Molecular Function:	unfolded protein binding [IDA] ATPase activity, coupled [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASETFEFQA EITQLMSLII NTVYSNKEIF LRELISNASD ALDKIRYKSL SDPKQLETEP  60
   61 DLFIRITPKP EQKVLEIRDS GIGMTKAELI NNLGTIAKSG TKAFMEALSA GADVSMIGQF 120
  121 GVGFYSLFLV ADRVQVISKS NDDEQYIWES NAGGSFTVTL DEVNERIGRG TILRLFLKDD 180
  181 QLEYLEEKRI KEVIKRHSEF VAYPIQLVVT KEVEKEVPIP EEEKKDEEKK DEEKKDEDDK 240
  241 KPKLEEVDEE EEKKPKTKKV KEEVQEIEEL NKTKPLWTRN PSDITQEEYN AFYKSISNDW 300
  301 EDPLYVKHFS VEGQLEFRAI LFIPKRAPFD LFESKKKKNN IKLYVRRVFI TDEAEDLIPE 360
  361 WLSFVKGVVD SEDLPLNLSR EMLQQNKIMK VIRKNIVKKL IEAFNEIAED SEQFEKFYSA 420
  421 FSKNIKLGVH EDTQNRAALA KLLRYNSTKS VDELTSLTDY VTRMPEHQKN IYYITGESLK 480
  481 AVEKSPFLDA LKAKNFEVLF LTDPIDEYAF TQLKEFEGKT LVDITKDFEL EETDEEKAER 540
  541 EKEIKEYEPL TKALKEILGD QVEKVVVSYK LLDAPAAIRT GQFGWSANME RIMKAQALRD 600
  601 SSMSSYMSSK KTFEISPKSP IIKELKKRVD EGGAQDKTVK DLTKLLYETA LLTSGFSLDE 660
  661 PTSFASRINR LISLGLNIDE DEETETAPEA STAAPVEEVP ADTEMEEVD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CPR6 FBA1 HSC82 HSP104 HSP82 STI1 TIF1, TIF2 YEF3
View Details	Krogan NJ, et al. (2006)		AHA1 BUD3 CNS1 CPR6 DIG2 DNM1 DSF1, YNR073C FRT2 GPA2 HOM6 HSC82 HSP104 HSP82 MBB1 MRPL50 PAM1 PPT1 QNS1 RMD8 RTS1 SMT3 STI1 STP4 TDH1 UTP20 YBL104C
View Details	Gavin AC, et al. (2002)		HSC82 HSP82 PPT1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ALG13	No Comments	Hazbun TR, et al. (2003)
View Run	PSF2	No Comments	Hazbun TR, et al. (2003)
View Run	UTP23	No Comments	Hazbun TR, et al. (2003)
View Run	SDO1	No Comments	Hazbun TR, et al. (2003)
View Run	DSN1	No Comments	Hazbun TR, et al. (2003)
View Run	PAN1	No Comments	Toshima J, et al (2006)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	HSP82	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..219]	PSI-BLAST	1236.39794	HSP90
2	View Details	[220..709]	Pfam	1000.0	Hsp90 protein No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.85

Protein Overview: HSP82

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: