Protein Overview: TCO89

General Information:
Name(s) found:	TCO89 / YPL180W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74583764\|sp\|Q08921.1\|TCO89_YEAST RecName: Full=Target of rapamycin complex 1 subunit TCO89; Short=TORC1 subunit TCO89; AltName: Full=89 kDa TOR complex 1 protein [NCBI NR] gi\|6325077\|ref\|NP_015145.1\| Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to [NCBI NR] pir\|\|S65192 hypothetical protein YPL180w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1370379\|emb\|CAA97887.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Target of rapamycin complex 1 subunit TCO89 OS=Saccharomyces cerevisiae GN=TCO89 PE=1 SV=1 [Swiss-Prot] TCO89 TOR complex 1, 89 kDa subunit [mips] Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin [SGD] >Q08921 (Q08921) Chromosome XVI reading frame ORF YPL180W [4932.FASTACCERV.txt] Chr XVI from 205247-207646 [hmr_yeast5_DM3-1_pombe.fasta] SHD7, Chr XVI from 205247-207646 [yeast_orfs2] TCO89 SGDID:S0006101, Chr XVI from 205247-207646, Verified ORF [yeastflysequest.fasta] Tco89p [Saccharomyces cerevisiae]gi\|1370379\|emb\|CAA97887.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|2132221\|pir\|\|S65192 hypothetical protein YPL180w - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] (Q08921) S.cerevisiae chromosome XVI reading frame ORF YPL180w [cerv4932.FASTAC] TCO89 SGDID:S000006101, Chr XVI from 205247-207646, Verified ORF, "Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin; Tco89p [Saccharom [nr-271106-contam.fasta] TCO89 SGDID:S000006101, Chr XVI from 205247-207646, Verified ORF, "Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Target of rapamycin complex 1 subunit TCO89 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TCO89 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] TCO89 SGDID:S000006101, Chr XVI from 205248-207647, Genome Release 64-1-1, Verified ORF, "Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin" [yeast-030211-contam.fasta] TCO89 SGDID:S000006101, Chr XVI from 205248-207647, Genome Release 64-2-1, Verified ORF, "Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1 complex regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin" [20141208_orf_trans.fasta] YPL180W SGDID:S000006101, Chr XVI from 205248-207647, Genome Release 64-1-1, Verified ORF, "Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	799 amino acids
Gene Ontology:
Cellular Component:	fungal-type vacuole membrane [IDA] extrinsic to vacuolar membrane [IDA] extrinsic to internal side of plasma membrane [IDA] TORC1 complex [IPI]
Biological Process:	glycerol metabolic process [IGI] fungal-type cell wall organization [IMP] response to salt stress [IMP] regulation of cell growth [IPI]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVHRGRTLKS DTDVTSLNAS TVSHQSKPFR QFSTRSRAKS NASFKGLRRV LTHDGTLDND  60
   61 YFNKHNVSQK CKSSDALFRK RTISGLNMTA LTRVKSNQGK RSASFHSPVH NTLLSPKNSS 120
  121 HSNTGTAGFG LKPRRSKSTQ SVLSLRDAQE SKKSESTTDE EVECFSEDNI EDGKVNNDKV 180
  181 IAEHVMPEEK KNVQQLNQNE LQSPDSIDEQ EEDKSGTDGK ENHRAVSLPL PHLSSNNYFG 240
  241 ESSHSIEHQK DGETSPSSIE TKLNATSVIN EEGQSKVTKE ADIDDLSSHS QNLRASLVKA 300
  301 GDNISEAPYD KEKKILDVGN TLAAHKSNQK PSHSDEQFDQ EDHIDAPRSN SSRKSDSSFM 360
  361 SLRRQSSKQH KLLNEEEDLI KPDDISSAGT KDIEGHSLLE NYAPNMILSQ STGVERRFEN 420
  421 SSSIQNSLGN EIHDSGEHMA SGDTFNELDD GKLRKSKKNG GRSQLGQNIP NSQSTFPTIA 480
  481 NIGSKDNNVP QHNFSTSISS LTNNLRRAAP ESFHGSRMNN IFHKKGNQNL LLRSNDLNKN 540
  541 SAAPASPLSN EHITSSTNSG SDANRQSNSG AKFNSFAQFL KSDGIDAESR TQRKLWLQRE 600
  601 NSIMDLSSQN DGSDSIFMAG NIDAKREFER ISHEYSNVKR FYNPLDEALL RVQPIITGNA 660
  661 NNIRKKSHND AQSIAHSSSD TDHKDEDDLL FTNYDKKFDD LYPHLASAKI QAVLSGIWKS 720
  721 ESYLFNKDVN PINKNRTTST NHSVGHTASQ NARNLLRGPM GSSTTLHHQR VINSLQPTTR 780
  781 AVNRRMENVG YMHTQPQQR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		AIM26 APC9 BEM3 FMP27 IRA1 MDM36 MID1 NUP42 REV1 SEC20 SML1 TCO89 TOP1 YDR396W YGL024W ZAP1 ZIM17
View Details	Qiu et al. (2008)		KOG1 LST8 TCO89 TOR1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	KOG1	No Comments	Reinke A, et al. (2004)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PDS1	TCO89	1	Unpublished Fields Lab Data
View Screen	MYO1	TCO89	1	Unpublished Fields Lab Data
View Screen	MDM30	TCO89	1	Unpublished Fields Lab Data
View Screen	UBC7	TCO89	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TCO89	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..568]	MSA	25.094998	View MSA. No confident structure predictions are available.
2	View Details	[569..799]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2