Protein Overview: RET3

General Information:
Name(s) found:	RET3 / YPL010W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|965083\|gb\|AAB68095.1\| Lpa7p [NCBI NR] gi\|683780\|emb\|CAA88376.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6325247\|ref\|NP_015315.1\| Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER; Ret3p [Saccharomyces cerevisiae] [NCBI NR] gi\|45270766\|gb\|AAS56764.1\| YPL010W [Saccharomyces cerevisiae] [NCBI NR] gi\|1706002\|sp\|P53600.1\|COPZ_YEAST RecName: Full=Coatomer subunit zeta; AltName: Full=Zeta-coat protein; Short=Zeta-COP [NCBI NR] gi\|1314066\|emb\|CAA95031.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S52521 coatomer protein zeta chain - yeast (Saccharomyces cerevisiae) [NCBI NR] Coatomer subunit zeta OS=Saccharomyces cerevisiae GN=RET3 PE=1 SV=1 [Swiss-Prot] RET3 Coatomer complex zeta chain [mips] Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER [SGD] (P53600) Coatomer zeta subunit (Zeta-coat protein) (Zeta-COP) [4932.FASTACCERV.txt] Chr XVI from 535013-535582 [hmr_yeast5_DM3-1_pombe.fasta] RET3, Chr XVI from 535013-535582 [yeast_orfs2] RET3 SGDID:S0005931, Chr XVI from 535013-535582, Verified ORF [yeastflysequest.fasta] RET3 SGDID:S0005931, Chr XVI from 535015-535584, Verified ORF [10112005cervSGD.fasta] Ret3p [Saccharomyces cerevisiae]gi\|683780\|emb\|CAA88376.1\| unknown [Saccharomyces cerevisiae]gi\|1314066\|emb\|CAA95031.1\| unknown [Saccharomyces cerevisiae]gi\|45270766\|gb\|AAS56764.1\| YPL010W [Saccharomyces cerevisiae]gi\|965083\|gb\|AAB68095.1\| Lpa7pgi\|107 [nrdb_11152004_Chlamydomonas] RET3 SGDID:S000005931, Chr XVI from 535015-535584, Verified ORF, "Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER; Ret3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YPL010W [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Lpa7p [nr-271106-contam.fasta] Coatomer subunit zeta (Zeta-coat protein) (Zeta-COP) [nr-271106-contam.fasta] [chemostatdb.fasta] Coatomer subunit zeta OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RET3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RET3 SGDID:S000005931, Chr XVI from 535018-535587, Genome Release 64-1-1, Verified ORF, "Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER" [yeast-030211-contam.fasta] RET3 SGDID:S000005931, Chr XVI from 535018-535587, Genome Release 64-2-1, Verified ORF, "Zeta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER" [20141208_orf_trans.fasta] TR1732\|c0_g1_i1\|g.1258 ORF TR1732\|c0_g1_i1\|g.1258 TR1732\|c0_g1_i1\|m.1258 type:complete len:190 (+) TR1732\|c0_g1_i1:68-637(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR3355\|c0_g1_i1\|g.2183 ORF TR3355\|c0_g1_i1\|g.2183 TR3355\|c0_g1_i1\|m.2183 type:complete len:190 (+) TR3355\|c0_g1_i1:70-639(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YPL010W SGDID:S000005931, Chr XVI from 535018-535587, Genome Release 64-1-1, Verified ORF, "Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_3225\|g.2302 ORF asmbl_3225\|g.2302 asmbl_3225\|m.2302 type:complete len:190 (+) asmbl_3225:100-669(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_3196\|g.2130 ORF ID=asmbl_3196\|g.2130 ID=asmbl_3196\|m.2130 type:complete len:190 (+) ID=asmbl_3196:84-653(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_3225\|g.2033 ORF ID=asmbl_3225\|g.2033 ID=asmbl_3225\|m.2033 type:complete len:190 (+) ID=asmbl_3225:100-669(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	189 amino acids
Gene Ontology:
Cellular Component:	COPI vesicle coat [IMP][ISS]
Biological Process:	retrograde vesicle-mediated transport, Golgi to ER [IMP]
Molecular Function:	protein binding [IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSLSLYTVQ AVLILDQQGE RIYAKYYQPP HRSDEGHQLL FNSVKKQKEF EKQLYRKTHK  60
   61 QDSEILIFED HLVLYKEYID ITIYLVASLE ENEIVLQQGF SAIRGALDLI LNSGMDKKNI 120
  121 QENYDMVLLA IDETIDNGVI LETDSNTIAS RVSKPPTNEP QMALDLDKGF LGAWGFAKSK 180
  181 FQERLQQGL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
View Details	(MIPS) Mewes HW, et al. (2004)		ARF1 COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
View Details	Krogan NJ, et al. (2006)		COP1 FCF2 FMP23 LCB5 PIP2 RET2 RET3 SCS2 SEC2 SEC21 SEC26 SEC27 SEC28
View Details	Gavin AC, et al. (2006)		COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
View Details	Gavin AC, et al. (2002)		COP1 GLO3 GPT2 ILV1 PGK1 RET2 RET3 SAM1 SCJ1 SEC21 SEC26 SEC27 SEC28 SEH1 TOS1
View Details	Qiu et al. (2008)		APL4 ARF1 COP1 GLO3 RET2 RET3 SEC21 SEC26 SEC27 VRG4

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	TMA23	RET3	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RET3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..189]	PSI-BLAST	42.221849	Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor; Mu2 adaptin (clathrin coat assembly protein AP50)

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.87

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2