Protein Overview: CDC31

General Information:
Name(s) found:	CDC31 / YOR257W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|729075\|sp\|P06704.2\|CDC31_YEAST RecName: Full=Cell division control protein 31; AltName: Full=Nucleoporin CDC31; AltName: Full=Nuclear pore protein CDC31 [NCBI NR] gi\|6324831\|ref\|NP_014900.1\| Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p; Cdc31p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S47549 cell division control protein CDC31 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|525276\|emb\|CAA52609.1\| Cdc31p [Saccharomyces cerevisiae] [NCBI NR] gi\|45270758\|gb\|AAS56760.1\| YOR257W [Saccharomyces cerevisiae] [NCBI NR] gi\|226887809\|pdb\|3FWC\|M Chain M, Sac3:sus1:cdc31 Complex [NCBI NR] gi\|226887805\|pdb\|3FWC\|I Chain I, Sac3:sus1:cdc31 Complex [NCBI NR] gi\|226887801\|pdb\|3FWC\|E Chain E, Sac3:sus1:cdc31 Complex [NCBI NR] gi\|226887797\|pdb\|3FWC\|A Chain A, Sac3:sus1:cdc31 Complex [NCBI NR] gi\|226887792\|pdb\|3FWB\|A Chain A, Sac3:sus1:cdc31 Complex [NCBI NR] gi\|1420581\|emb\|CAA99479.1\| CDC31 [Saccharomyces cerevisiae] [NCBI NR] Cell division control protein 31 OS=Saccharomyces cerevisiae GN=CDC31 PE=1 SV=2 [Swiss-Prot] (P06704) Cell division control protein 31 (Nucleoporin CDC31) (Nuclear pore protein CDC31) [Swiss-Prot] CDC31 Spindle pole body component, centrin [mips] Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation [SGD] (P06704) Cell division control protein 31 [4932.FASTACCERV.txt] Chr XV from 811006-811491 [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 811005-811490 [yeastorf3.fasta] CDC31, Chr XV from 811005-811490 [yeast_orfs2] CDC31 SGDID:S0005783, Chr XV from 811006-811491, Verified ORF [yeastflysequest.fasta] Cdc31p [Saccharomyces cerevisiae]gi\|1420581\|emb\|CAA99479.1\| CDC31 [Saccharomyces cerevisiae]gi\|525276\|emb\|CAA52609.1\| Cdc31p [Saccharomyces cerevisiae]gi\|45270758\|gb\|AAS56760.1\| YOR257W [Saccharomyces cerevisiae]gi\|626119\|pir\|\|S47549 cell division con [nrdb_11152004_Chlamydomonas] CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] CDC31 SGDID:S000005783, Chr XV from 811008-811493, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" [yeast-contam.fasta] CDC31 SGDID:S000005783, Chr XV from 811008-811493, Verified ORF, "Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] YOR257W [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p; Cdc31p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Cdc31p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] CDC31 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Cell division control protein 31 (Nucleoporin CDC31) (Nuclear pore protein CDC31) [nr-271106-contam.fasta] CDC31 SGDID:S000005783, Chr XV from 811008-811493, Verified ORF, "Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Cell division control protein 31 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CDC31 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CDC31 SGDID:S000005783, Chr XV from 811008-811493, Genome Release 64-1-1, Verified ORF, "Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation" [yeast-030211-contam.fasta] CDC31 SGDID:S000005783, Chr XV from 811008-811493, Genome Release 64-2-1, Verified ORF, "Calcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation" [20141208_orf_trans.fasta] YOR257W SGDID:S000005783, Chr XV from 811008-811493, Genome Release 64-1-1, Verified ORF, "Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	161 amino acids
Gene Ontology:
Cellular Component:	half bridge of spindle pole body [TAS] nuclear pore [TAS]
Biological Process:	microtubule nucleation [IPI] spindle pole body duplication in nuclear envelope [IMP]
Molecular Function:	structural constituent of cytoskeleton [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKNRSSLQS GPLNSELLEE QKQEIYEAFS LFDMNNDGFL DYHELKVAMK ALGFELPKRE  60
   61 ILDLIDEYDS EGRHLMKYDD FYIVMGEKIL KRDPLDEIKR AFQLFDDDHT GKISIKNLRR 120
  121 VAKELGETLT DEELRAMIEE FDLDGDGEIN ENEFIAICTD S

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		BBP1 CDC31 CMD1 CNM67 KAR1 NUD1 SPC105 SPC110 SPC24 SPC25 SPC29 SPC42 SPC72
View Details	Krogan NJ, et al. (2006)		CDC31 HOS3 PSA1 PTK2 RPL1A, RPL1B
View Details	Qiu et al. (2008)		CDC31 CMD1 CNM67 KAR1 MPS2 MPS3 NUD1 NUF2 SFI1 SPC105 SPC110 SPC19 SPC24 SPC29 SPC42 SPC72 SPC97 SPC98 STU1 TID3 TUB4

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#33 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#27 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps3-5	Keck JM, et al. (2011)
View Run	MLP2	#21 Asynchronous Prep2-IMAC Phosphopeptide enrichment A1	Keck JM, et al. (2011)
View Run	MLP2	#22 Asynchronous Prep2-IMAC Phosphopeptide enrichment B1-2	Keck JM, et al. (2011)
View Run	MLP2	#34 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#35 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#01 Alpha factor Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#16 Mitotic-New search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#05 Alpha Factor Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#07 Alpha Factor Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	None Entered	#18 Mitotic Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPE1	CDC31	1	Unpublished Fields Lab Data
View Screen	PEX13	CDC31	1	Unpublished Fields Lab Data
View Screen	UBC12	CDC31	1	Unpublished Fields Lab Data
View Screen	CIN4	CDC31	1	Unpublished Fields Lab Data
View Screen	TPT1	CDC31	1	Unpublished Fields Lab Data
View Screen	HSH49	CDC31	1	Unpublished Fields Lab Data
View Screen	SEC17	CDC31	1	Unpublished Fields Lab Data
View Screen	KAP104	CDC31	1	Unpublished Fields Lab Data
View Screen	KAP104	CDC31	1	Unpublished Fields Lab Data
View Screen	RPN5	CDC31	1	Unpublished Fields Lab Data
View Screen	MSN5	CDC31	1	Unpublished Fields Lab Data
View Screen	RPN11	CDC31	1	Unpublished Fields Lab Data
View Screen	AIM13	CDC31	1	Unpublished Fields Lab Data
View Screen	TOS8	CDC31	1	Unpublished Fields Lab Data
View Screen	RSR1	CDC31	1	Unpublished Fields Lab Data
View Screen	NAS6	CDC31	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

No microscopy data found in the PDR for this protein.

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..89]	PSI-BLAST	287.165202	Calmodulin
2	View Details	[90..161]	PSI-BLAST	287.165202	Calmodulin

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2