Protein Overview: STE4

General Information:
Name(s) found:	STE4 / YOR212W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324786\|ref\|NP_014855.1\| G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains [NCBI NR] pir\|\|S60939 GTP-binding protein beta chain STE4 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1420495\|emb\|CAA99427.1\| STE4 [Saccharomyces cerevisiae] [NCBI NR] gi\|1346108\|sp\|P18851.2\|GBB_YEAST RecName: Full=Guanine nucleotide-binding protein subunit beta [NCBI NR] gi\|1050764\|emb\|CAA63175.1\| STE4 [Saccharomyces cerevisiae] [NCBI NR] Guanine nucleotide-binding protein subunit beta OS=Saccharomyces cerevisiae GN=STE4 PE=1 SV=2 [Swiss-Prot] STE4 GTP-binding protein beta subunit of the pheromone pathway [mips] G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats [SGD] (P18851) Guanine nucleotide-binding protein beta subunit [4932.FASTACCERV.txt] Chr XV from 742910-744181 [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 742909-744180 [yeastorf3.fasta] STE4, Chr XV from 742909-744180 [yeast_orfs2] STE4 SGDID:S0005738, Chr XV from 742910-744181, Verified ORF [yeastflysequest.fasta] Ste4p [Saccharomyces cerevisiae]gi\|1420495\|emb\|CAA99427.1\| STE4 [Saccharomyces cerevisiae]gi\|1050764\|emb\|CAA63175.1\| STE4 [Saccharomyces cerevisiae]gi\|2133158\|pir\|\|S60939 GTP-binding protein beta chain STE4 - yeast (Saccharomyces cerevisiae)gi\|1346108 [nrdb_11152004_Chlamydomonas] STE4 SGDID:S000005738, Chr XV from 742910-744181, Verified ORF, "G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] STE4 SGDID:S000005738, Chr XV from 742911-744182, Verified ORF, "G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized [yeast-contam.fasta] Guanine nucleotide-binding protein subunit beta [nr-271106-contam.fasta] STE4 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats; Ste4p [Sacchar [nr-271106-contam.fasta] STE4 SGDID:S000005738, Chr XV from 742911-744182, Verified ORF, "G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Guanine nucleotide-binding protein subunit beta OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=STE4 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] STE4 SGDID:S000005738, Chr XV from 742910-744181, Genome Release 64-1-1, Verified ORF, "G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats" [yeast-030211-contam.fasta] STE4 SGDID:S000005738, Chr XV from 742910-744181, Genome Release 64-2-1, Verified ORF, "G protein beta subunit; forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats" [20141208_orf_trans.fasta] TR6471\|c0_g1_i1\|g.4565 ORF TR6471\|c0_g1_i1\|g.4565 TR6471\|c0_g1_i1\|m.4565 type:complete len:424 (+) TR6471\|c0_g1_i1:249-1520(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YOR212W SGDID:S000005738, Chr XV from 742910-744181, Genome Release 64-1-1, Verified ORF, "G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_2487\|g.1680 ORF ID=asmbl_2487\|g.1680 ID=asmbl_2487\|m.1680 type:complete len:424 (+) ID=asmbl_2487:248-1519(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	423 amino acids
Gene Ontology:
Cellular Component:	heterotrimeric G-protein complex [IDA][IMP] mating projection [IDA] plasma membrane [IDA]
Biological Process:	heterotrimeric G-protein complex cycle [IMP] invasive growth in response to glucose limitation [IMP] pheromone-dependent signal transduction involved in conjugation with cellular fusion [IGI][IPI]
Molecular Function:	signal transducer activity [IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH AQINKAKHKI  60
   61 QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW SRDSKRILSA SQDGFMLIWD 120
  121 SASGLKQNAI PLDSQWVLSC AISPSSTLVA SAGLNNNCTI YRVSKENRVA QNVASIFKGH 180
  181 TCYISDIEFT DNAHILTASG DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS 240
  241 NTFASCGSDG YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR 300
  301 SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ GVVSLDFSAS 360
  361 GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS PDGLAVCTGS WDSTMKIWSP 420
  421 GYQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		GPA1 STE18 STE4
View Details	Krogan NJ, et al. (2006)		AIR1 AIR2 MTR4 PAP2 PSO2 RPL18A, RPL18B STE4 TRF5
View Details	Ho Y, et al. (2002)		ADE17 AHA1 ARP2 CCT2 CCT3 CCT5 CCT6 GCD11 GPA1 PGM2 RNQ1 SEC53 STE4 TCP1 YHR033W
View Details	Qiu et al. (2008)		BEM1 CBK1 CDC24 CDC42 FUS3 GPA1 SHO1 STE18 STE2 STE3 STE4 STE5 STE7

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	STE4	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..423]	PSI-BLAST	10094.522879	No description for 1scgB_ was found.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Protein Overview: STE4

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: