Protein Overview: LEO1

General Information:
Name(s) found:	LEO1 / YOR123C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324697\|ref\|NP_014766.1\| Component of the Paf1 complex, which associates with RNA polymerase II and is involved in histone methylation; Leo1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S45363 LEO1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|585391\|sp\|P38439.1\|LEO1_YEAST RecName: Full=RNA polymerase-associated protein LEO1 [NCBI NR] gi\|443971\|emb\|CAA54391.1\| Leo1 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1420322\|emb\|CAA99322.1\| LEO1 [Saccharomyces cerevisiae] [NCBI NR] gi\|1164967\|emb\|CAA64042.1\| YOR3278c [Saccharomyces cerevisiae] [NCBI NR] gi\|1050827\|emb\|CAA62122.1\| ORF O3278 [Saccharomyces cerevisiae] [NCBI NR] RNA polymerase-associated protein LEO1 OS=Saccharomyces cerevisiae GN=LEO1 PE=1 SV=1 [Swiss-Prot] LEO1 Extremely hydrophilic protein [mips] Component of the Paf1 complex, which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition [SGD] (P38439) LEO1 protein [4932.FASTACCERV.txt] Chr XV from 554570-553176, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 553175-554569, reverse complement [yeastorf3.fasta] Chr XV from 553176-554570, reverse complement [yeastorf4.fasta] LEO1, Chr XV from 553175-554569, reverse complement [yeast_orfs2] LEO1 SGDID:S0005649, Chr XV from 554570-553176, reverse complement, Verified ORF [yeastflysequest.fasta] Component of the Paf1 complex, which associates with RNA polymerase II and is involved in histone methylation [Saccharomyces cerevisiae]gi\|1420322\|emb\|CAA99322.1\| LEO1 [Saccharomyces cerevisiae]gi\|1164967\|emb\|CAA64042.1\| YOR3278c [Saccharomyces cerevisi [nrdb_11152004_Chlamydomonas] LEO1 SGDID:S000005649, Chr XV from 554570-553176, reverse complement, Verified ORF, "Component of the Paf1 complex, which associates with RNA polymerase II and is involved in histone methylation" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] LEO1 SGDID:S000005649, Chr XV from 554571-553177, reverse complement, Verified ORF, "Component of the Paf1 complex, which associates with RNA polymerase II and is involved in histone methylation" [yeast-contam.fasta] LEO1 SGDID:S000005649, Chr XV from 554571-553177, reverse complement, Verified ORF, "Component of the Paf1 complex, which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] LEO1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Component of the Paf1 complex, which associates with RNA polymerase II and is involved in histone methylation; Leo1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Leo1 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ORF O3278 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RNA polymerase-associated protein LEO1 [nr-271106-contam.fasta] YOR3278c [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] RNA polymerase-associated protein LEO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LEO1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] LEO1 SGDID:S000005649, Chr XV from 554570-553176, Genome Release 64-1-1, reverse complement, Verified ORF, "Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay" [yeast-030211-contam.fasta] LEO1 SGDID:S000005649, Chr XV from 554570-553176, Genome Release 64-2-1, reverse complement, Verified ORF, "Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay" [20141208_orf_trans.fasta] YOR123C SGDID:S000005649, Chr XV from 554570-553176, Genome Release 64-1-1, reverse complement, Verified ORF, "Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	464 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] transcription elongation factor complex [IPI] Cdc73/Paf1 complex [IPI][TAS]
Biological Process:	histone methylation [IMP] telomere maintenance [IMP] transcription from RNA polymerase II promoter [IMP] RNA elongation from RNA polymerase II promoter [IPI]
Molecular Function:	RNA polymerase II transcription elongation factor activity [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSESPQDQP QKEQISNNVG VTTNSTSNEE TSRSQDDNVK EVNGNDDTKE EEQEEDAELD  60
   61 DLFGDDNDDD DDDDVKKSET EKSDSDSDED DEGENINHRS RHRESLGLDD DEAEEQAMYT 120
  121 RKFYGEDANN FSDQDETTHT FKEENVELVR HIIPSKANVN ETASHNEIFY ARIPNFLTID 180
  181 PIPFDPPSFE AKVNERASNS ASREDQLDDR LIDENTVRWR YSRDKDQHVF KESNTQIVQW 240
  241 SDGTYSLKVG EECTDILVND TSNTFLTVSH DQQELIQCYE GGEIKKTLMF IPTSTNSKIH 300
  301 QKLSKAVIRR NQRQSKGPGT YIVSMDPEVE KKELERKQSQ ILRDRRRRQL KEKEKQESPD 360
  361 AAFETGFRKQ NSPTTYGASR RNEYEEDDFL VDDDEEEEAA FDDEEDDNEE EEEEEDADEE 420
  421 NASRLRNLKR EGAAMYREEE EEEKDRSETK RRRVAVIEDD EDED

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CDC73 CKA1 CKA2 CKB1 CKB2 CTR9 LEO1 PAF1 POB3 RTF1 SPT16
View Details	Krogan NJ, et al. (2006)		ARA2 AYT1 BRO1 CDC73 CTR9 GLO4 HHT1, HHT2 LEO1 PAF1 PML39 POB3 PSH1 RTF1 SEN1 SPT16 YHR035W
View Details	Gavin AC, et al. (2002)		CDC73 CKA1 CKA2 CKB1 CKB2 CTR9 IML1 LEO1 LYS12 PAF1 POB3 RTF1 SPT16
View Details	Qiu et al. (2008)		CDC73 CHD1 CTR9 ELA1 ELC1 LEO1 PAF1 RTF1 SPT5 TFB5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	LEO1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..181]	MSA	2.010996	View MSA. No confident structure predictions are available.
2	View Details	[182..464]	deduced	N/A	No confident structure predictions are available.
3	View Details	[166..240]	MSA	2.044993	View MSA. No confident structure predictions are available.
4	View Details	[241..464]	MSA	2.044977	View MSA. No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: LEO1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: