Protein Overview: RPC31

General Information:
Name(s) found:	RPC31 / YNL151C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|RNBY3C DNA-directed RNA polymerase (EC 2.7.7.6) III chain C31 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6324178\|ref\|NP_014248.1\| RNA polymerase III subunit C31; contains HMG-like C-terminal domain; Rpc31p [Saccharomyces cerevisiae] [NCBI NR] gi\|4377\|emb\|CAA35866.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|133370\|sp\|P17890.1\|RPC7_YEAST RecName: Full=DNA-directed RNA polymerase III subunit RPC7; Short=RNA polymerase III subunit C7; AltName: Full=DNA-directed RNA polymerase III 31 kDa polypeptide; Short=C31 [NCBI NR] gi\|1302108\|emb\|CAA96038.1\| RPC31 [Saccharomyces cerevisiae] [NCBI NR] gi\|1050801\|emb\|CAA63288.1\| RPC31 [Saccharomyces cerevisiae] [NCBI NR] DNA-directed RNA polymerase III 31 kDa polypeptide (EC 2.7.7.6) (C31) - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] DNA-directed RNA polymerase III subunit RPC7 OS=Saccharomyces cerevisiae GN=RPC31 PE=1 SV=1 [Swiss-Prot] RPC31 DNA-directed RNA polymerase III, 31 KD subunit [mips] RNA polymerase III subunit C31 [SGD] RPC31, Chr XIV from 347763-348518, reverse complement [yeast_orfs2] Chr XIV from 347763-348518, reverse complement [yeastorf3.fasta] Chr XIV from 348518-347763, reverse complement [yeastorf5.fasta] (P17890) DNA-directed RNA polymerase III 31 kDa polypeptide (EC 2.7.7.6) (C31) [4932.FASTACCERV.txt] RPC31 SGDID:S0005095, Chr XIV from 348521-347766, reverse complement, Verified ORF [yeastflysequest.fasta] RNA polymerase III subunit C31; contains HMG-like C-terminal domain [Saccharomyces cerevisiae]gi\|1050801\|emb\|CAA63288.1\| RPC31 [Saccharomyces cerevisiae]gi\|1302108\|emb\|CAA96038.1\| RPC31 [Saccharomyces cerevisiae]gi\|4377\|emb\|CAA35866.1\| unnamed protein [nrdb_11152004_Chlamydomonas] RPC31 SGDID:S000005095, Chr XIV from 348523-347768, reverse complement, Verified ORF, "RNA polymerase III subunit C31; contains HMG-like C-terminal domain" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] DNA-directed RNA polymerase III 31 kDa polypeptide (C31) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RPC31 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RNA polymerase III subunit C31; contains HMG-like C-terminal domain; Rpc31p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] DNA-directed RNA polymerase III subunit RPC7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPC31 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RPC31 SGDID:S000005095, Chr XIV from 348521-347766, Genome Release 64-1-1, reverse complement, Verified ORF, "RNA polymerase III subunit C31" [yeast-030211-contam.fasta] RPC31 SGDID:S000005095, Chr XIV from 348521-347766, Genome Release 64-2-1, reverse complement, Verified ORF, "RNA polymerase III subunit C31" [20141208_orf_trans.fasta] TR1813\|c0_g1_i1\|g.1231 ORF TR1813\|c0_g1_i1\|g.1231 TR1813\|c0_g1_i1\|m.1231 type:complete len:252 (+) TR1813\|c0_g1_i1:2380-3135(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YNL151C SGDID:S000005095, Chr XIV from 348521-347766, Genome Release 64-1-1, reverse complement, Verified ORF, "RNA polymerase III subunit C31" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_5701\|g.4005 ORF asmbl_5701\|g.4005 asmbl_5701\|m.4005 type:complete len:252 (+) asmbl_5701:68-823(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_6147\|g.4479 ORF asmbl_6147\|g.4479 asmbl_6147\|m.4479 type:complete len:252 (+) asmbl_6147:2396-3151(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_5698\|g.3455 ORF ID=asmbl_5698\|g.3455 ID=asmbl_5698\|m.3455 type:complete len:252 (+) ID=asmbl_5698:68-823(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_6174\|g.3675 ORF ID=asmbl_6174\|g.3675 ID=asmbl_6174\|m.3675 type:complete len:252 (+) ID=asmbl_6174:2397-3152(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	251 amino acids
Gene Ontology:
Cellular Component:	DNA-directed RNA polymerase III complex [TAS]
Biological Process:	transcription from RNA polymerase III promoter [TAS]
Molecular Function:	DNA-directed RNA polymerase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSYRGGSRG GGSNYMSNLP FGLGYGDVGK NHITEFPSIP LPINGPITNK ERSLAVKYIN  60
   61 FGKTVKDGPF YTGSMSLIID QQENSKSGKR KPNIILDEDD TNDGIERYSD KYLKKRKIGI 120
  121 SIDDHPYNLN LFPNELYNVM GINKKKLLAI SKFNNADDVF TGTGLQDENI GLSMLAKLKE 180
  181 LAEDVDDAST GDGAAKGSKT GEGEDDDLAD DDFEEDEDEE DDDDYNAEKY FNNGDDDDYG 240
  241 DEEDPNEEAA F

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		MAF1 RET1 RPB10 RPB5 RPB8 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO26 RPO31
View Details	(MIPS) Mewes HW, et al. (2004)		RET1 RPB10 RPB5 RPB8 RPC10 RPC19 RPC25 RPC31 RPC34 RPC40 RPC82 RPO26 RPO31
View Details	Krogan NJ, et al. (2006)		BDP1 CDC14 COY1 FAR7 MAF1 RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPC10 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO31 YDL144C YIR024C
View Details	Gavin AC, et al. (2006)		RET1 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO31
View Details	Gavin AC, et al. (2002)		AMS1 ASC1 CDC14 DHH1 FBA1 MAF1 RET1 RPA12 RPA135 RPA190 RPA43 RPA49 RPB10 RPB5 RPB8 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO26 RPO31 RSC8 SRO7
View Details	Qiu et al. (2008)		RET1 RPA12 RPA34 RPA43 RPB10 RPB11 RPB3 RPB4 RPB5 RPB7 RPB8 RPC10 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO26 RPO31

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC4	RPC31	1	Unpublished Fields Lab Data
View Screen	YJR141W	RPC31	1	Unpublished Fields Lab Data
View Screen	PET111	RPC31	1	Unpublished Fields Lab Data
View Screen	MSA1	RPC31	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..251]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2