Protein Overview: NBP1

General Information:
Name(s) found:	NBP1 / YLR457C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|717076\|gb\|AAB64721.1\| Nbp1p [Saccharomyces cerevisiae] [NCBI NR] gi\|6323491\|ref\|NP_013562.1\| Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; Nbp1p [Sac [NCBI NR] gi\|1709231\|sp\|P52919.1\|NBP1_YEAST RecName: Full=NAP1-binding protein [NCBI NR] pir\|\|S59416 hypothetical protein YLR457c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1272509\|dbj\|BAA07740.1\| Nap1-binding protein [Saccharomyces cerevisiae] [NCBI NR] NAP1-binding protein OS=Saccharomyces cerevisiae GN=NBP1 PE=1 SV=1 [Swiss-Prot] NBP1 Nap1p-binding protein [mips] Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex [SGD] NBP1, Chr XII from 1055808-1056767, reverse complement [yeast_orfs2] Chr XII from 1055808-1056767, reverse complement [yeastorf3.fasta] Chr XII from 1056767-1055808, reverse complement [yeastorf5.fasta] (P52919) NAP1-binding protein [4932.FASTACCERV.txt] NBP1 SGDID:S0004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF [yeastflysequest.fasta] Nap1p Binding Protein ; Nap1p binding protein [Saccharomyces cerevisiae]gi\|1709231\|sp\|P52919\|NBP1_YEAST NAP1-binding proteingi\|1363733\|pir\|\|S59416 hypothetical protein YLR457c - yeast (Saccharomyces cerevisiae)gi\|717076\|gb\|AAB64721.1\| Nbp1p [Saccharomy [nrdb_11152004_Chlamydomonas] NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of the mitotic apparatus containing a coiled-coil domain, essential for the G2/M transition" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NBP1 SGDID:S000004449, Chr XII from 1056769-1055810, reverse complement, Verified ORF, "Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle [yeast-contam.fasta] Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; Nbp1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Nbp1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NAP1-binding protein [nr-271106-contam.fasta] Nap1-binding protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NBP1 SGDID:S000004449, Chr XII from 1056769-1055810, reverse complement, Verified ORF, "Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex" [yeast-bovrabprots.fasta] [chemostatdb.fasta] NAP1-binding protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NBP1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NBP1 SGDID:S000004449, Chr XII from 1056771-1055812, Genome Release 64-1-1, reverse complement, Verified ORF, "Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex" [yeast-030211-contam.fasta] NBP1 SGDID:S000004449, Chr XII from 1056771-1055812, Genome Release 64-2-1, reverse complement, Verified ORF, "Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YLR457C SGDID:S000004449, Chr XII from 1056771-1055812, Genome Release 64-1-1, reverse complement, Verified ORF, "Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	319 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] central plaque of spindle pole body [IDA] spindle pole body [IDA]
Biological Process:	spindle pole body duplication in nuclear envelope [IGI][IMP][IPI]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLKSVQGLWK DFFGIRDDGR KREYGSLDEV RKRSALRSRR KQMRPTGKSV LKRPRKVTDR  60
   61 KTEEKIRTNR RKTPKRRLTK IFQTIRDVFS NDNENMSKMQ NVCGDMTRIL KKRSQGRPSY 120
  121 MDTDTAKSRI LRSDAFKRKI SELKYNKQRI SELRSGSSDG SSGKDRNQSL YLDREILLQR 180
  181 QIKKRDEKIK ALESKLQSLQ EALNYSNEKY RILEDLLDSS NIHPSYTKSR RTMSNLAREN 240
  241 DEIKPLKIDL SPSPIRRTNS LFTSSPMKTY NRDGNIPEMQ PLQENISPAC PTPPYRSRET 300
  301 EKEDETLSPI SVDFSSYLS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		HSK3 NBP1 SFI1 SPC29 SPC42

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#34 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#01 Alpha factor Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#16 Mitotic-New search criteria	Keck JM, et al. (2011)
View Run	MLP2	#15 Mitotic 2nd-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#05 Alpha Factor Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#07 Alpha Factor Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	None Entered	#18 Mitotic Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	FRT2	NBP1	2	Unpublished Fields Lab Data
View Screen	SST2	NBP1	2	Unpublished Fields Lab Data
View Screen	IMP4	NBP1	2	Unpublished Fields Lab Data
View Screen	FAR10	NBP1	1	Unpublished Fields Lab Data
View Screen	MEC3	NBP1	1	Unpublished Fields Lab Data
View Screen	CTF3	NBP1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NBP1 and SPC110	Sundin BA, et al. (2004)
View Data	NBP1 and SPC110	Sundin BA, et al. (2004)
View Data	YPR174C and NBP1	Sundin BA, et al. (2004)
View Data	YPR174C and NBP1	Sundin BA, et al. (2004)
View Data	NBP1	Huh WK, et al. (2003)
View Data	NBP1 and SPC42	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..161]	deduced	N/A	No confident structure predictions are available.
2	View Details	[162..319]	MSA	1.002967	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2