Protein Overview: SEC3

General Information:
Name(s) found:	SEC3 / YER008C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320845\|ref\|NP_010924.1\| Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark f [NCBI NR] gi\|603600\|gb\|AAB64541.1\| Sec3p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S41794 SEC3 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|464482\|sp\|P33332.1\|SEC3_YEAST RecName: Full=Exocyst complex component SEC3; AltName: Full=Protein PSL1 [NCBI NR] gi\|347716\|gb\|AAB49380.1\| Psl1p [NCBI NR] Exocyst complex component SEC3 OS=Saccharomyces cerevisiae GN=SEC3 PE=1 SV=1 [Swiss-Prot] SEC3 Component of exocyst complex [mips] Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion [SGD] SEC3, Chr V from 167807-171817, reverse complement [yeast_orfs2] Chr V from 167807-171817, reverse complement [yeastorf3.fasta] Chr V from 171817-167807, reverse complement [yeastorf5.fasta] (P33332) Exocyst complex component SEC3 (PSL1 protein) [4932.FASTACCERV.txt] SEC3 SGDID:S0000810, Chr V from 171817-167807, reverse complement, Verified ORF [yeastflysequest.fasta] Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion [Saccharomyces [nrdb_11152004_Chlamydomonas] SEC3 SGDID:S000000810, Chr V from 171817-167807, reverse complement, Verified ORF, "Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of activ [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Psl1p [nr-271106-contam.fasta] Exocyst complex component SEC3 (Protein PSL1) [nr-271106-contam.fasta] Sec3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion; Sec3p [Sacchar [nr-271106-contam.fasta] SEC3 SGDID:S000000810, Chr V from 171817-167807, reverse complement, Verified ORF, "Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] SEC3 SGDID:S000000810, Chr V from 171817-167807, reverse complement, Verified ORF, "Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion" [yeast-bovrabprots.fasta] [chemostatdb.fasta] SEC3 SGDID:S000000810, Chr V from 171817-167807, reverse complement, Verified ORF, "Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion" [SGDyeast.20101221.fasta] Exocyst complex component SEC3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SEC3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SEC3 SGDID:S000000810, Chr V from 171818-167808, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion" [yeast-030211-contam.fasta] SEC3 SGDID:S000000810, Chr V from 171818-167808, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress" [20141208_orf_trans.fasta] YER008C SGDID:S000000810, Chr V from 171818-167808, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1336 amino acids
Gene Ontology:
Cellular Component:	incipient cellular bud site [IDA] cellular bud tip [IDA] site of polarized growth [IDA] cellular bud neck [IDA] exocyst [IPI][TAS]
Biological Process:	vesicle fusion [TAS] bipolar cellular bud site selection [IMP] vesicle docking during exocytosis [TAS] Golgi to plasma membrane transport [TAS] cytokinesis [IMP] exocytosis [IDA]
Molecular Function:	protein binding [IGI][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MRSSKSPFKR KSHSRETSHD ENTSFFHKRT ISGSSAHHSR NVSQGAVPSS APPVSGGNYS  60
   61 HKRNVSRASN SSQTSNFLAE QYERDRKAII NCCFSRPDHK TGEPPNNYIT HVRIIEDSKF 120
  121 PSSRPPPDSK LENKKKRLLI LSAKPNNAKL IQIHKARENS DGSFQIGRTW QLTELVRVEK 180
  181 DLEISEGFIL TMSKKYYWET NSAKERTVFI KSLITLYIQT FEGHVPELVN WDLSLFYLDE 240
  241 RSYQRAVITN RPGSVSPIKS PTSNFTTNTT QSVGSVPFSA PTERTRRSET ESVNPVSTPA 300
  301 SVEYHAGMKS LNKAPYSSNS TLNEVNKRYE LEQQQQQEEA ELRRLEEQKR LQLQKENEMK 360
  361 RLEEERRIKQ EERKRQMELE HQRQLEEEER KRQMELEAKK QMELKRQRQF EEEQRLKKER 420
  421 ELLEIQRKQR EQETAERLKK EEQEALAKKE EEEKSKRNKV DNESYTQEIN GKVDNLLEDL 480
  481 NAVLAEETET TPTMQNGTYV PERSTARAHD QLKKPLNIAK VESLGGSDLN DSISLSDEIA 540
  541 GLNTSNLSGE DQDEKNDLSF EKGDEVRYSN NFEGEAPHVY HEVSIIQEEA PAVSQKLILP 600
  601 EENNESEALI ESKEEIKTME NIDDEVLLEI LTDINWSIED DADSMIERID LRLAETEYLF 660
  661 NQNLLSLQKI GPNIRPYEDK VNDECHRIIP TLSLFLMEMS NFSNDIENVE SQDNGLQVES 720
  721 ANKKLLWNTL DELLKTVSLD EISLNQLLEC PIREKNLPWM ENQLNLLLKA FQAIGSDGNE 780
  781 VEYNLREISG LKQRLQFYEK VTKIFLNRIV EEMQKKFSNI RGQDISHDQM IRILTTLLIF 840
  841 SPLILFCKEI SQKSYQAIVE NWNVSIQPVY MELWTKKISQ LQGIDTNDEK MNELSLSQLL 900
  901 NEWDTFRKER KTNDINPVFK NSFSLLTECL QTMRQECIVY QNFVEVFFHI SSKHNFEEYI 960
  961 KHFNDPDAPP ILLDTVKVMQ SDREAAVIET QLVSRIFQPI VTRLSSYFVE LVKAEPTVAP1020
 1021 ALTFYLENEI KSLESSNHEF LLSAVTRMYT QIKQVWSDNV EEQVLHFERI SNATTNGEIL1080
 1081 PGILDLPVGL KNSEDLFQFA KRSMDIKDTD EGYESIELMN SSFRKLSIAA TRSITHKEVN1140
 1141 SSINPNLSDT AALNNDYMET ISLLVNSNWL TEMLSMLNFN KDGIFDTSLQ NVKKVFDVEK1200
 1201 ESYASFLLRD TMPKLTAFVY GVSNIIENTN NVNMTNPSRW AAYSRQNLEN ILLAYTSHEI1260
 1261 ETLVKRLHTH MVNDFGYHQE NAINNVLCDK LWSCIQGQTV SLYLKLYTVI DKHYRGTNIR1320
 1321 FTKNDIISAF EEYKNA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		EXO84 SEC10 SEC15 SEC3 SEC5 SEC6 SEC8
View Details	(MIPS) Mewes HW, et al. (2004)		EXO70 SEC10 SEC15 SEC3 SEC5 SEC6 SEC8
View Details	Gavin AC, et al. (2006)		EXO84 SEC10 SEC3 SEC5 SEC6 SEC8
View Details	Gavin AC, et al. (2002)		ACE2 ALT2 BCK1 CDC11 DRE2 ECM30 ENP2 EXO84 IML1 MNL1 MTR4 MYO2 RGC1 SEC10 SEC3 SEC5 SEC6 SEC8 UBP15 VPS9 YBL044W YJR098C YNL313C
View Details	Qiu et al. (2008)		EXO70 EXO84 GYL1 SEC10 SEC15 SEC3 SEC5 SEC6 SEC8

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	SEC10	No Comments	De Craene, J., et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	SEC3	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..160]	MSA	2.865996	View MSA. No confident structure predictions are available.
2	View Details	[161..274] [308..340]	ORFEUS	10.32	No description for 1lu7A was found.
3	View Details	[275..307] [341..382]	ORFEUS	10.32	No description for 1lu7A was found.
4	View Details	[383..777]	PSI-BLAST	18.09691	Heavy meromyosin subfragment
5	View Details	[778..968]	MSA	14.252997	View MSA. No confident structure predictions are available.
6	View Details	[969..1336]	deduced	N/A	No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: SEC3

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: