Sec3p is a spatial landmark for polarized secretion in budding yeast

Cell. 1998 Feb 20;92(4):559-71. doi: 10.1016/s0092-8674(00)80948-4.

Abstract

Exocytosis in yeast occurs at plasma membrane subdomains whose locations vary with the cell cycle, but the primary protein determinants of these sites are unknown. A functional fusion of Sec3 protein with green fluorescent protein (Sec3-GFP) localizes to the site of polarized exocytosis for each cell-cycle stage, where it colocalizes with Sec4p and Sec8p. Sec3-GFP localization is independent of secretory pathway function, of the actin and septin cytoskeletons, and of the polarity establishment proteins. We propose that Sec3p is a spatial landmark defining sites of exocytosis. Polarized secretion would result from the coupling of actin-dependent vesicle targeting with Sec3p-dependent establishment of the vesicle fusion site.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / analysis
  • Actins / metabolism
  • Carrier Proteins*
  • Cell Cycle Proteins / analysis
  • Cell Cycle Proteins / genetics
  • Cell Polarity / physiology*
  • Cytoskeletal Proteins*
  • Cytoskeleton / chemistry
  • Cytoskeleton / metabolism
  • Exocytosis / physiology*
  • Fungal Proteins / analysis
  • Fungal Proteins / metabolism*
  • GTP Phosphohydrolases
  • GTP-Binding Proteins / analysis
  • Green Fluorescent Proteins
  • Indicators and Reagents
  • Intracellular Membranes / physiology
  • Luminescent Proteins
  • Membrane Proteins
  • Mutation
  • Profilins
  • Proteins / analysis
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors / analysis
  • Vesicular Transport Proteins
  • rab GTP-Binding Proteins*

Substances

  • Actins
  • CDC12 protein, S cerevisiae
  • CDC3 protein, S cerevisiae
  • Carrier Proteins
  • Cell Cycle Proteins
  • Cytoskeletal Proteins
  • Fungal Proteins
  • Indicators and Reagents
  • Luminescent Proteins
  • Membrane Proteins
  • Profilins
  • Proteins
  • SEC3 protein, S cerevisiae
  • SEC8 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • Vesicular Transport Proteins
  • cdc10 protein, S pombe
  • Green Fluorescent Proteins
  • CDC10 protein, S cerevisiae
  • GTP Phosphohydrolases
  • GTP-Binding Proteins
  • SEC4 protein, S cerevisiae
  • rab GTP-Binding Proteins