Protein Overview: AFR1

General Information:
Name(s) found:	AFR1 / YDR085C [SGD]
Description(s) found: Found 35 descriptions. SHOW ALL	Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	620 amino acids
Gene Ontology:
Cellular Component:	mating projection base [IDA]
Biological Process:	cell morphogenesis involved in conjugation [IMP] regulation of G-protein coupled receptor protein signaling pathway [IMP][IPI] pheromone-dependent signal transduction involved in conjugation with cellular fusion [IGI][IMP][IPI]
Molecular Function:	receptor signaling protein activity [IMP][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEGSYLSAQE NQPIPERLIP RSNSTSNLFA LSSTFSKLNV RNDADYNYSN PNKKRHIYSG  60
   61 EIDCRSVTAA RKFPVRSCSM TAAQQRKRTA LFTVRERNSY HEGFNNDQDY VSQYQKPQYT 120
  121 FGVYKELTPY QLQRSKMKRS FQFPNGEIYK PKLDGKCTHS LKKPELNSRD SSLFKFSEKK 180
  181 GRNLSKDFVG PHNGTSVIHI PPNDTGYGVN SLELNTSVPS TIKSSVSSTS PISAVNTLTS 240
  241 LPESQTDDDD GYENKTVTIS YCFENTVNEK HGSHIEKLDL STKEKTKPTT NSGLFDRKKK 300
  301 TILGTEKYRC IKSQSKLKLG SVLKKLWRTS GNSNTKHGKK DTKRRRIPID DMVTHSDGNS 360
  361 EAENDIELMD ANLDGIEFDD DETLMDTDSI FDDLLSKEND KYDLRRRQLE IRQKLHETSH 420
  421 NDDGKVSFRD TEKHNVNEGL IDKTIIEEFS KLGEYIIDTR NQPPPRSSKR PSLDDNESAR 480
  481 YFYNISTDLR QSLSGPISLP MHVGNDMVNR LRNDWEYIRF EDRRNSLPDS SFDKVETPPK 540
  541 PIKKDVRFAK EVCLASTWSS NAYERANPEF IMNRHRLLWM MKVHPSMNSA MNEIKLELNS 600
  601 YKKNEMVVHE NSKCFTHYLI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		AAT2 ACH1 ACO1 AFR1 AHP1 ARC1 ARF1 ATP3 CAR2 CDC33 CLU1 COF1 COR1 CPR1 CYR1 CYS4 EGD1 ERG13 ERG6 FPR1 FRS2 GND1 GND2 GRX1 GUS1 HEF3 HHF1, HHF2 LRO1 LSP1 MET6 NTF2 OLA1 OYE2 PFK1 PIL1 PRM2 RNR2 RSN1 SAH1 SCP160 SEC53 SEN15 SES1 SNU13 SOD1 TEF4 THS1 TIF1, TIF2 TMA19 TSA2 UBA1 VMA4 VMA5 WTM1 YNK1
View Details	Qiu et al. (2008)		AFR1 STE5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HMT1	AFR1	1	Unpublished Fields Lab Data
View Screen	GLC7	AFR1	1	Unpublished Fields Lab Data
View Screen	UBP6	AFR1	1	Unpublished Fields Lab Data
View Screen	SPT21	AFR1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..620]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2