Protein Overview: MRC1

General Information:
Name(s) found:	MRC1 / YCL061C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|34978392\|sp\|P25588.3\|MRC1_YEAST RecName: Full=Mediator of replication checkpoint protein 1; AltName: Full=DNA replication checkpoint mediator MRC1 [NCBI NR] gi\|14588900\|emb\|CAC42953.1\| hypothetical protein [Saccharomyces cerevisiae] [NCBI NR] gi\|10383754\|ref\|NP_009871.2\| S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphoryla [NCBI NR] Mediator of replication checkpoint protein 1 OS=Saccharomyces cerevisiae GN=MRC1 PE=1 SV=3 [Swiss-Prot] MRC1 Mediator of the Replication Checkpoint [mips] S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres [SGD] MRC1, Chr III from 18816-22106, reverse complement [yeast_orfs2] Chr III from 18816-22106, reverse complement [yeastorf3.fasta] Chr III from 22106-18816, reverse complement [yeastorf5.fasta] (P25588) DNA replication checkpoint mediator MRC1 [4932.FASTACCERV.txt] MRC1 SGDID:S0000566, Chr III from 22106-18816, reverse complement, Verified ORF [yeastflysequest.fasta] Mrc1p [Saccharomyces cerevisiae]gi\|14588900\|emb\|CAC42953.1\| hypothetical protein [Saccharomyces cerevisiae]gi\|34978392\|sp\|P25588\|MRC1_YEAST Mediator of replication checkpoint protein 1 (DNA replication checkpoint mediator MRC1) [nrdb_11152004_Chlamydomonas] (P25588) Mediator of replication checkpoint protein 1 (DNA replication checkpoint mediator MRC1) [cerv4932.FASTAC] MRC1 SGDID:S000000566, Chr III from 22106-18816, reverse complement, Verified ORF, "S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a re [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MRC1 SGDID:S000000566, Chr III from 22106-18816, reverse complement, Verified ORF, "S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex w [yeast-200209-contam.fasta] hypothetical protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mediator of replication checkpoint protein 1 (DNA replication checkpoint mediator MRC1) [nr-271106-contam.fasta] S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; protein involve [nr-271106-contam.fasta] MRC1 SGDID:S000000566, Chr III from 22106-18816, reverse complement, Verified ORF, "S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres" [yeast-bovrabprots.fasta] MRC1 SGDID:S000000566, Chr III from 22106-18816, reverse complement, Verified ORF, "S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; protein involved in replication checkpoint" [yeast-contam_custom.fasta] [chemostatdb.fasta] Mediator of replication checkpoint protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MRC1 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MRC1 SGDID:S000000566, Chr III from 22106-18816, Genome Release 64-1-1, reverse complement, Verified ORF, "S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres" [yeast-030211-contam.fasta] MRC1 SGDID:S000000566, Chr III from 22106-18816, Genome Release 64-2-1, reverse complement, Verified ORF, "S-phase checkpoint protein required for DNA replication; couples DNA helicase and DNA polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; with Hog1p defines a novel S-phase checkpoint that permits eukaryotic cells to prevent conflicts between DNA replication and transcription; protects uncapped telomeres; degradation via Dia2p help cells resume cell cycle" [20141208_orf_trans.fasta] YCL061C SGDID:S000000566, Chr III from 22106-18816, Genome Release 64-1-1, reverse complement, Verified ORF, "S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1096 amino acids
Gene Ontology:
Cellular Component:	replication fork [IDA] nucleus [IDA] nuclear chromosome [IPI]
Biological Process:	DNA replication checkpoint [IGI][IMP ][IPI] telomere maintenance [IMP ] chromatin silencing at silent mating-type cassette [IGI][IMP] chromatin silencing at telomere [IGI][IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDDALHALSS LTAKKRTTTY KKVAVPILDE NDNTNGNGPN DIDNPPELTG NGFLFANATL  60
   61 NRVKNRLEGK KAPEQNHNNG KDRSENSLPT QLISNLYDGG EELEKSEVKD NSYSEKNVSS 120
  121 SFTQTQRIPV SIQQDKVFNV PIHSVNDGKP TQLIKEDGLV NETSQALKTP LTTGRPGATQ 180
  181 RIDSSGATSQ TQPIKSIEPQ SQIITTSSNH SNALSPKIPI IPTELIGTSP LFQSIQNRGP 240
  241 DTQMDVPPQT AHDEDKTQAI GIPQATHQEQ KTQIDTVAQT LQDEVPHTLK IREIQSELAS 300
  301 EDSKREKARN VEYKKPQKPI PTKKFFSKES FLADFDDSSS NEDDDIKLEN AHPKPVQNDD 360
  361 ELHENKSVEL NLTDETRINE KRVPLLSSYA NNLKREIDSS KCITLDLDSD SDEYGDDDMD 420
  421 SIKLSKDESV LPISQLSKAT ILNLKARLSK QNQKLSQRPN KSKDPKVDHN VLLNTLRKAS 480
  481 RKQILDHQKE VIETKGLKLE DMAKEKEIVE NLLEQEILRN KRIRQKEKRR EKLEENDFQL 540
  541 NAHDSGSDSG SESSGFALSG NEIADYESSG SENDNRRESD SEKEDDEIIL KQKKSHHVKH 600
  601 IINESDSDTE VEAKPKEKAD ESLPKRIAIN LGHYGDNIGE DTDKFQETNV LDTQNIEEVM 660
  661 AERNTIENEV KDDVYVNEEA DEAIRRQLID KEKLQLKQKE KEHEAKIKEL KKRGVTNFFE 720
  721 MEAEESEDEW HGIGGADGEG SDDYDSDLEK MIDDYSKNNF NPHEIREMLA AENKEMDIKM 780
  781 INKILYDIKN GGFRNKRAKN SLELELSDDD EDDVLQQYRL KRRELMRKRR LEIGDDAKLV 840
  841 KNPKSSAFFE SMVEDIIEYK NPFGAEEEYN LDITSTATDL DTQDNSINVG DNTGNNEQKP 900
  901 VDQKNKKVII SEDFVQKSLS FLKSNNYEDF ETDKELSRIQ HGNDEAIEDL YTLKQNSSIK 960
  961 SFTNSQTDST TSKTVNTIID LEKRPEDEDE VENGDTSLVG VFKHPSIIKS FASRTDINDK1020
 1021 FKEGNKTVKI LKSYKTVGSS KASITYMGKT RKLIAPKRKT EGSHRYHHDH HNKKMKMKTK1080
 1081 TKSNKLFESG QDSFDN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Hazbun TR, et al. (2003)		AIM25 DED81 GWT1 MRC1 SEC63 SEC7
View Details	Krogan NJ, et al. (2006)		ADE2 BSC2 CWH41 DBF4 DIG1 ECM29 HAL5 LAA1 MRC1 OPI9 PEX32 PRP5 PTH2 RGA2 RMA1 RPN3 SIR1 STE12 THR1 TLG2 VPS45 YDL199C YEL057C YPL035C
View Details	Qiu et al. (2008)		DPB11 ELG1 MCM4 MRC1 POL30

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	GWT1	No Comments	Hazbun TR, et al. (2003)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RNR3	MRC1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MRC1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..242]	deduced	N/A	No confident structure predictions are available.
2	View Details	[243..564]	MSA	1.003862	View MSA. No confident structure predictions are available.
3	View Details	[565..639]	deduced	N/A	No confident structure predictions are available.
4	View Details	[640..893]	MSA	1.004921	View MSA. No confident structure predictions are available.
5	View Details	[894..1096]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2