Protein Overview: Mdr50-PA

General Information:
Name(s) found:	Mdr50-PA / FBpp0086666 [FlyBase]
Description(s) found: SHOW ONLY BEST	gi\|85726357\|ref\|NP_523740.3\| multi drug resistance 50 [Drosophila melanogaster] [NCBI NR] gi\|84795750\|gb\|AAF58271.3\| multi drug resistance 50 [Drosophila melanogaster] [NCBI NR] gi\|60678007\|gb\|AAX33510.1\| LP14331p [Drosophila melanogaster] [NCBI NR] The gene Multi drug resistance 50 is referred to in FlyBase by the symbol Dmel\Mdr50 (CG8523, FBgn0010241). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is 2R:10134471..10140102. It has the cytological map location 50E6. Based on sequence similarity, it is predicted to have molecular function</span>: ATPase activity, coupled; multidrug transporter activity. Based on sequence similarity, it is predicted to be involved in the biological process</span>: drug transport. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. [FlyBase] (AE003815) Mdr50 gene product [Drosophila melanogaster] [yeastflysequest.fasta] type=protein; loc=2R:join(10134575..10134690,10135505..10135730,10135800..10136083,10136261..10138220,10138280..10138585,10138650..10138789,10138859..10139420,10139483..10139629,10139692..10139892); ID=FBpp0086666; name=Mdr50-PA; parent=FBgn0010241,FBtr00 [fly-100309-contam.fasta] CG8523-PA [Drosophila melanogaster] [nr-271106-contam.fasta] LP14331p [Drosophila melanogaster] [nr-271106-contam.fasta] Multi drug resistance 50 CG8523-PA [Drosophila melanogaster] [nr-271106-contam.fasta] type=protein; loc=2R:join(10134575..10134690,10135505..10135730,10135800..10136083,10136261..10138220,10138280..10138585,10138650..10138789,10138859..10139420,10139483..10139629,10139692..10139892); ID=FBpp0086666; name=Mdr50-PA; parent=FBgn0010241,FBtr0087537; dbxref=FlyBase_Annotation_IDs:CG8523-PA,FlyBase:FBpp0086666,GB_protein:AAF58271.2,GB_protein:AAF58271.3,REFSEQ:NP_523740,GB_protein:AAF58271; MD5=93abfd737c4c63da3a32f868efbc4527; length=1313; release=r5.16; species=Dmel; [fly-contam-His-TEV-Tub4complex.fasta] type=protein; loc=2R:join(10134575..10134690,10135505..10135730,10135800..10136083,10136261..10138220,10138280..10138585,10138650..10138789,10138859..10139420,10139483..10139629,10139692..10139892); ID=FBpp0086666; name=Mdr50-PA; parent=FBgn0010241,FBtr0087537; dbxref=FlyBase_Annotation_IDs:CG8523-PA,FlyBase:FBpp0086666,GB_protein:AAF58271.2,GB_protein:AAF58271.3,REFSEQ:NP_523740,GB_protein:AAF58271; MD5=93abfd737c4c63da3a32f868efbc4527; length=1313; release=r5.21; species=Dmel; [Other_drosophila_na_09-09-2009_reversed.fasta] LP14331p OS=Drosophila melanogaster GN=Mdr50 PE=2 SV=1 [UniProt_drosophila_07-01-2011_reversed.fasta] type=protein; loc=2R:join(10134575..10134690,10135505..10135730,10135800..10136083,10136261..10138220,10138280..10138585,10138650..10138789,10138859..10139420,10139483..10139629,10139692..10139892); ID=FBpp0086666; name=Mdr50-PA; parent=FBgn0010241,FBtr0087537; dbxref=FlyBase_Annotation_IDs:CG8523-PA,FlyBase:FBpp0086666,GB_protein:AAF58271.2,GB_protein:AAF58271.3,REFSEQ:NP_523740,GB_protein:AAF58271,FlyMine:FBpp0086666,modMine:FBpp0086666; MD5=93abfd737c4c63da3a32f868efbc4527; length=1313; release=r5.46; species=Dmel; [fly-062812-sfps-sandra-contam.fasta] type=protein; loc=2R:join(14247070..14247185,14248000..14248225,14248295..14248578,14248756..14250715,14250775..14251080,14251145..14251284,14251354..14251915,14251978..14252124,14252187..14252387); ID=FBpp0086666; name=Mdr50-PA; parent=FBgn0010241,FBtr0087537; dbxref=FlyBase_Annotation_IDs:CG8523-PA,FlyBase:FBpp0086666,GB_protein:AAF58271.2,GB_protein:AAF58271.3,REFSEQ:NP_523740,GB_protein:AAF58271,FlyMine:FBpp0086666,modMine:FBpp0086666; MD5=93abfd737c4c63da3a32f868efbc4527; length=1313; release=r6.02; species=Dmel; [fly-2014-05-contam.fasta] type=protein; loc=2R:join(14247070..14247185,14248000..14248225,14248295..14248578,14248756..14250715,14250775..14251080,14251145..14251284,14251354..14251915,14251978..14252124,14252187..14252387); ID=FBpp0086666; name=Mdr50-PA; parent=FBgn0010241,FBtr0087537; dbxref=FlyBase_Annotation_IDs:CG8523-PA,FlyBase:FBpp0086666,GB_protein:AAF58271.2,GB_protein:AAF58271.3,REFSEQ:NP_523740,GB_protein:AAF58271,FlyMine:FBpp0086666,modMine:FBpp0086666; MD5=93abfd737c4c63da3a32f868efbc4527; length=1313; release=r6.07; species=Dmel; [fly-08202015-contam.fasta] LP14331p OS=Drosophila melanogaster GN=Mdr50 PE=1 SV=1 [DROME.fasta.20160308] FBpp0086666 type=protein; loc=2R:join(14247070..14247185,14248000..14248225,14248295..14248578,14248756..14250715,14250775..14251080,14251145..14251284,14251354..14251915,14251978..14252124,14252187..14252387); ID=FBpp0086666; name=Mdr50-PA; parent=FBgn0010241,FBtr0087537; dbxref=FlyBase_Annotation_IDs:CG8523-PA,FlyBase:FBpp0086666,GB_protein:AAF58271.2,GB_protein:AAF58271.3,REFSEQ:NP_523740,GB_protein:AAF58271,FlyMine:FBpp0086666,modMine:FBpp0086666; MD5=93abfd737c4c63da3a32f868efbc4527; length=1313; release=r6.09; species=Dmel; [dmel-all-translation-r6.09.fasta] [fly-122215-dmel-6-09-contam.fasta] type=protein; loc=2R:join(14247070..14247185,14248000..14248225,14248295..14248578,14248756..14250715,14250775..14251080,14251145..14251284,14251354..14251915,14251978..14252124,14252187..14252387); ID=FBpp0086666; name=Mdr50-PA; parent=FBgn0010241,FBtr0087537; dbxref=FlyBase_Annotation_IDs:CG8523-PA,FlyBase:FBpp0086666,GB_protein:AAF58271.2,GB_protein:AAF58271.3,REFSEQ:NP_523740,GB_protein:AAF58271,UniProt/TrEMBL:Q5BI62,FlyMine:FBpp0086666,modMine:FBpp0086666; MD5=93abfd737c4c63da3a32f868efbc4527; length=1313; release=r6.14; species=Dmel; [fly-dmel-jan2017-sandracontam.fasta]
Organism:	Drosophila melanogaster
Length:	1313 amino acids
Gene Ontology:
Cellular Component:	integral to membrane [IEA]
Biological Process:	drug transport [ISS] transmembrane transport [IEA]
Molecular Function:	ATP binding [IEA] ATPase activity, coupled to transmembrane movement of substances [IEA] drug transmembrane transporter activity [ISS][NAS] ATPase activity, coupled [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTVKNGDVVK DDVNSRSQYK TNIVLGAKLE DSDRDRKSFE PNKSKKKSKH DESDASDEED  60
   61 GSQYHEDVKQ VSYFQLFRYA TKKDRALYVI GLLSAVATGL TTPANSLIFG NLANDMIDLG 120
  121 GLLESGKSYR ADDAISTLLL DKVRQFSLQN TYIGIIMLVC SYLSITCFNY AAHSQILTIR 180
  181 SKFFRSILHQ DMKWYDFNQS GEVASRMNED LSKMEDGLAE KVVMFVHYLV AFVGSLVLAF 240
  241 VKGWQLSLVC LTSLPLTFIA MGLVAVATSR LAKKEVTMYA GAAVVAEGAL SGIRTVKAFE 300
  301 GEAKEVAAYK ERVVAAKILN IKRNMFSGIG FGLLWFFIYA SYALAFWYGV GLVIKGYHEP 360
  361 AYENYDAGTM ITVFFSVMMG SMNIGMAAPY IEAFGIAKGA CAKVFHIIEQ IPEINPIDGE 420
  421 GKKLNEPLTT IEFKEVEFQY PTRPEVSILN KLNLKIHRGQ TVALVGPSGC GKSTCIQLVQ 480
  481 RFYDPQAGNL LFNGTNLKDL DINWLRSRIG VVGQEPILFA TSIYENIRYG REDATREEIE 540
  541 AAAAAANAAI FIKKLPKGYD TLVGERGAQL SGGQKQRIAI ARALIRDPEI LLLDEATSAL 600
  601 DTASEAKVQA ALEKVSAGRT TIIVAHRLST VRRADRIVVI NKGEVVESGT HQELMELKDH 660
  661 YFNLVTTQLG EDDGSVLSPT GDIYKNFDIK DEDEEEIKVL SEDEDEDVMV TDEKNKKKKK 720
  721 KKVKDPNEVK PMLEVMKMNK PEWLQIAVGC ISSVIMGCAM PIFAVLFGSI LQILSVKDND 780
  781 QYVRENSNQY SLYFLIAGIV VGIATFLQIY FFGIAGERLT ERLRGLMFEA MLRQEVAWFD 840
  841 DKANGTGSLC ARLSGDAAAV QGATGQRIGT IVQSISTLAL GIALSMYYEW SLGLVALAFT 900
  901 PFILIAFYMQ RTLMAKENMG SAKTMENCTK LAVEVVSNIR TVASLGREEM FHQNYIGMLI 960
  961 PAVEISKRNT HFRGLVYGLA RSLMFFAYAA CMYYGTWCVI HRGILFGDVF KVSQALIMGT1020
 1021 ASIANALAFA PNMQKGVSAA KTIFTFLRRQ PSIVDRPGVS RDPWHSEGYV RFDKVKFSYP1080
 1081 TRSEIQVLKG LELAVSKGQK IALVGPSGCG KSTCIQLIQR FYDVDEGATL IDECDVRNVS1140
 1141 MTNLRNQLGI VSQEPILFDR TIRENISYGD NARNVTDQEI ISACKKSNIH EFIANLPLGY1200
 1201 DTRMGEKGAQ LSGGQKQRIA IARALIRNPK IMLLDEATSA LDAESEKVVQ DALDAASEGR1260
 1261 TTISIAHRLS TVVHSDVIFV FENGLVCEAG DHKQLLANRG LYYTLYKLQS GAM

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..426]	FFAS03	4.26	No description for 2hydA was found.
2	View Details	[427..715]	PSI-BLAST	62.09691	No description for 2cbzA was found.
3	View Details	[716..1313]	PSI-BLAST	144.0	No description for 2hydA was found.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
lipid transporter activity	6.95325922226092	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	5.4467861893359	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	5.44654566127945	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	5.44654566127945	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	5.43909190876515	bayes_pls_golite062009
primary active transmembrane transporter activity	5.4304944906989	bayes_pls_golite062009
active transmembrane transporter activity	5.38112407181712	bayes_pls_golite062009
ATPase activity, coupled	4.87869064889244	bayes_pls_golite062009
ATPase activity	4.60773943958528	bayes_pls_golite062009
nucleoside-triphosphatase activity	4.38591214726007	bayes_pls_golite062009
pyrophosphatase activity	4.32673473878039	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	4.31087891816858	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4.31037981322306	bayes_pls_golite062009
transporter activity	3.47953169973151	bayes_pls_golite062009
hydrolase activity	3.11706952898443	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.96171085039592	bayes_pls_golite062009
transmembrane transporter activity	2.94175800357966	bayes_pls_golite062009
substrate-specific transporter activity	2.90743799425416	bayes_pls_golite062009
ATP binding	2.83986218503994	bayes_pls_golite062009
adenyl ribonucleotide binding	2.79195527951815	bayes_pls_golite062009
adenyl nucleotide binding	2.74359323245512	bayes_pls_golite062009
purine ribonucleotide binding	2.55506944753783	bayes_pls_golite062009
ribonucleotide binding	2.55426312645972	bayes_pls_golite062009
purine nucleotide binding	2.51344800975739	bayes_pls_golite062009
nucleotide binding	2.48057252852174	bayes_pls_golite062009
binding	1.66695393603756	bayes_pls_golite062009
catalytic activity	0.372934161904337	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
lipid transporter activity	7.47642599298853	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	5.57844095962502	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	5.57813579764186	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	5.57813579764186	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	5.5689620139321	bayes_pls_golite062009
primary active transmembrane transporter activity	5.55992639965745	bayes_pls_golite062009
active transmembrane transporter activity	5.50005167063544	bayes_pls_golite062009
ATPase activity, coupled	4.98276715440477	bayes_pls_golite062009
peptide transporter activity	4.7442815698413	bayes_pls_golite062009
anion transmembrane-transporting ATPase activity	4.71935767060308	bayes_pls_golite062009
ATPase activity	4.69795387297193	bayes_pls_golite062009
peptide-transporting ATPase activity	4.63590351019108	bayes_pls_golite062009
nucleoside-triphosphatase activity	4.45923622153365	bayes_pls_golite062009
pyrophosphatase activity	4.39781350157199	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	4.38133275795019	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4.38085303582726	bayes_pls_golite062009
oligopeptide-transporting ATPase activity	4.09365342567844	bayes_pls_golite062009
transporter activity	3.71239480053329	bayes_pls_golite062009
dipeptide transporter activity	3.65103732558911	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	3.57926427233638	bayes_pls_golite062009
ion transmembrane transporter activity	3.35436118751376	bayes_pls_golite062009
phosphate transmembrane-transporting ATPase activity	3.20894563245332	bayes_pls_golite062009
inorganic anion transmembrane transporter activity	3.16975154423199	bayes_pls_golite062009
hydrolase activity	3.14498892233323	bayes_pls_golite062009
transmembrane transporter activity	3.13960906549884	bayes_pls_golite062009
anion transmembrane transporter activity	3.13913638619234	bayes_pls_golite062009
substrate-specific transporter activity	3.1318898376276	bayes_pls_golite062009
cation transmembrane transporter activity	3.08390071341252	bayes_pls_golite062009
iron ion transmembrane transporter activity	3.05511942104215	bayes_pls_golite062009
ATP binding	3.01346604299372	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	3.00745742755195	bayes_pls_golite062009
drug transmembrane transporter activity	2.97119034312001	bayes_pls_golite062009
adenyl ribonucleotide binding	2.96271391116269	bayes_pls_golite062009
adenyl nucleotide binding	2.91131545957759	bayes_pls_golite062009
metal ion transmembrane transporter activity	2.88476360771975	bayes_pls_golite062009
purine ribonucleotide binding	2.70262534541085	bayes_pls_golite062009
ribonucleotide binding	2.70178191749524	bayes_pls_golite062009
amine-transporting ATPase activity	2.69529191205818	bayes_pls_golite062009
organic acid transmembrane transporter activity	2.67957846350199	bayes_pls_golite062009
carboxylic acid transmembrane transporter activity	2.67815672695824	bayes_pls_golite062009
purine nucleotide binding	2.6583477492092	bayes_pls_golite062009
nucleotide binding	2.62374702628143	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	2.61311581579476	bayes_pls_golite062009
sterol transporter activity	2.48473697371886	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	2.40292717311506	bayes_pls_golite062009
amine transmembrane transporter activity	2.29486577878686	bayes_pls_golite062009
	2.20174656498665	bayes_pls_golite062009
phosphate transmembrane transporter activity	2.15147988861177	bayes_pls_golite062009
oligopeptide transporter activity	2.05580564974083	bayes_pls_golite062009
L-aspartate transmembrane transporter activity	2.05274761921715	bayes_pls_golite062009
transition metal ion transmembrane transporter activity	1.82177108193332	bayes_pls_golite062009
organic anion transmembrane transporter activity	1.76999970080179	bayes_pls_golite062009
protein transporter activity	1.71214667007262	bayes_pls_golite062009
binding	1.69327117652379	bayes_pls_golite062009
amino acid transmembrane transporter activity	1.59627719545436	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	1.58971273330352	bayes_pls_golite062009
phosphonate transmembrane transporter activity	1.40269234722336	bayes_pls_golite062009
cation channel activity	1.33585529285301	bayes_pls_golite062009
molybdate transmembrane-transporting ATPase activity	1.28313088935613	bayes_pls_golite062009
molybdate ion transmembrane transporter activity	1.28313088935613	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of ions	1.23799860254751	bayes_pls_golite062009
potassium channel activity	1.17288958041707	bayes_pls_golite062009
sulfate transmembrane-transporting ATPase activity	1.1220946941463	bayes_pls_golite062009
voltage-gated cation channel activity	1.00384984515681	bayes_pls_golite062009
voltage-gated ion channel activity	0.889208591820696	bayes_pls_golite062009
motor activity	0.87979065815418	bayes_pls_golite062009
voltage-gated channel activity	0.871525745291603	bayes_pls_golite062009
ion channel activity	0.848261056209476	bayes_pls_golite062009
GTPase activity	0.83849854735032	bayes_pls_golite062009
channel activity	0.806331380134838	bayes_pls_golite062009
passive transmembrane transporter activity	0.806331380134838	bayes_pls_golite062009
substrate-specific channel activity	0.799997700906738	bayes_pls_golite062009
copper ion transmembrane transporter activity	0.798934081014025	bayes_pls_golite062009
di-, tri-valent inorganic cation transmembrane transporter activity	0.767424210804186	bayes_pls_golite062009
gated channel activity	0.650050847753837	bayes_pls_golite062009
cholesterol transporter activity	0.644421039250979	bayes_pls_golite062009
guanyl nucleotide binding	0.640772201693186	bayes_pls_golite062009
guanyl ribonucleotide binding	0.63468044350616	bayes_pls_golite062009
GTP binding	0.621689261700018	bayes_pls_golite062009
protein transmembrane transporter activity	0.46552249844591	bayes_pls_golite062009
branched-chain aliphatic amino acid transmembrane transporter activity	0.442220326523999	bayes_pls_golite062009
catalytic activity	0.368034275052766	bayes_pls_golite062009
neutral amino acid transmembrane transporter activity	0.227570438294493	bayes_pls_golite062009
signal recognition particle binding	0.20866768220789	bayes_pls_golite062009
protein binding	0.193768631623192	bayes_pls_golite062009
amino acid-transporting ATPase activity	0.147644650870465	bayes_pls_golite062009
nucleic acid binding	0.144904409289829	bayes_pls_golite062009
microtubule motor activity	0.11674780318211	bayes_pls_golite062009
sulfate transmembrane transporter activity	0.104477131120205	bayes_pls_golite062009
cation-transporting ATPase activity	0.0536898657672154	bayes_pls_golite062009
P-P-bond-hydrolysis-driven protein transmembrane transporter activity	0.00571427458217011	bayes_pls_golite062009
macromolecule transmembrane transporter activity	0.00571427458217011	bayes_pls_golite062009

Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Protein Overview: Mdr50-PA

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: