Protein Overview: BUD14

General Information:
Name(s) found:	BUD14 / YAR014C [SGD]
Description(s) found: Found 18 descriptions. SHOW ALL	Protein involved in bud-site selection, Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern [SGD]
Organism:	Saccharomyces cerevisiae
Length:	707 amino acids
Gene Ontology:
Cellular Component:	incipient cellular bud site [IDA] cellular bud tip [IDA] cellular bud neck [IDA]
Biological Process:	cytoskeleton organization [IGI][IMP][IPI] cell morphogenesis during vegetative growth [IGI] regulation of transcription, DNA-dependent [IDA][IGI][IMP]
Molecular Function:	protein phosphatase type 1 regulator activity [IDA][IMP][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSNKEEHVDE TSASGVKEVS SIAARHDNGY APSLITSTSG MDSFQSHALL NDPTLIEDYS  60
   61 DIINNRPTSG SKLTLGNEDS ESMGGSVVVT PTSNKSSPFN SKLNILSNAA EKGHDVLRNR 120
  121 DDDKELEEEN VEKHMHSNSK RDQRHYKENS SELPDSYDYS DSEFEDNLER RLQEIETDSV 180
  181 DSADKDEVHF SVNNTMNPDV DDFSDGLKYA ISEDEDEEEN YSDDDDFDRK FQDSGFQGEK 240
  241 DDLEEENDDY QPLSPPRELD PDKLYALYAF NGHDSSHCQL GQDEPCILLN DQDAYWWLVK 300
  301 RITDGKIGFA PAEILETFPE RLARLNCWKN ENMSSQSVAS SDSKDDSISS GNKNQSDAES 360
  361 IIPTPALNGY GKGNKSVSFN DVVGYADRFI DDAIEDTSLD SNDDGGEGNG QSYDDDVDND 420
  421 KETKVTHRDE YTEAKLNFAK FQDDDTSDVV SDVSFSTSLN TPLNVKKVRR QDNKNESEPK 480
  481 TSSSKDREDD YNANRYVGQE KSEPVDSDYD TDLKKVFEAP RMPFANGMAK SDSQNSLSTI 540
  541 GEFSPSSSEW TNESPSTPIV EESSSIPSSR AIRTFTYIMQ NRKLRDNKRG KHRGQIQASL 600
  601 GSSGGMPNQT DAEQPKEELE KHHSTPEEEK QSTLSLHSSS EEDFYMDEQR AVSSASINSS 660
  661 LSGSRALSNT NMSDPASKPN SLVQHLYAPV FDRMDVLMKQ LDEIIRK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BNI4 BUD14 GIP3 GLC7 GLC8 HER1 PPZ1 PUF3 SDS22 SOL1 SOL2 YLR257W YPI1
View Details	Gavin AC, et al. (2002)		AMN1 ASC1 BEM2 BUD14 KIN3 MIS1 SCP160 SKI3 SSZ1 TIF4631 ZUO1
View Details	Ho Y, et al. (2002)		ABP1 ACO1 AIM41 ATP4 BUD14 CAR2 CMP2 COR1 CRZ1 CYS4 DCP1 DCP2 DNM1 DOG1 EDC3 EDE1 EGD2 ENP1 GAS1 GCN3 GIS4 GPH1 HHF1, HHF2 HRR25 HSP104 HXT7 HYP2 IPP1 KEL1 KEL2 KIN2 KRE6 LOC1 LSP1 LTV1 MDH1 MDS3 MGE1 NSA1 NUG1 OYE2 PEX19 PIL1 PIN4 POP2 PTC4 PUF3 RIO2 RPC19 RPM2 SAP185 SAP190 SAS10 SEC2 SEC23 SES1 SFB3 SGM1 SIT4 TEF4 TFC4 TMA19 TSR1 UBA1 VMA4 YBR225W YER138C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BUD14	YLR072W	2	Unpublished Fields Lab Data
View Screen	BUD14	SPO20	2	Unpublished Fields Lab Data
View Screen	BUD14	MAD2	2	Unpublished Fields Lab Data
View Screen	BUD14	NMD5	2	Unpublished Fields Lab Data
View Screen	BUD14	DRS1	2	Unpublished Fields Lab Data
View Screen	RAS2	BUD14	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BUD14	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..186]	MSA	2.097997	View MSA. No confident structure predictions are available.
2	View Details	[187..259]	deduced	N/A	No confident structure predictions are available.
3	View Details	[260..419]	PSI-BLAST	33.0	Fyn proto-oncogene tyrosine kinase, SH3 domain; Tyrosine kinase Fyn
4	View Details	[420..491]	deduced	N/A	No confident structure predictions are available.
5	View Details	[492..600]	deduced	N/A	No confident structure predictions are available.
6	View Details	[601..707]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
binding	2.48735671726158	bayes_pls_golite062009
protein binding	2.14586566310919	bayes_pls_golite062009
cytoskeletal protein binding	2.02807082768447	bayes_pls_golite062009
actin binding	1.61769239689139	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.29663830666504	bayes_pls_golite062009
transcription regulator activity	1.28186497060277	bayes_pls_golite062009
nucleic acid binding	1.22075469862949	bayes_pls_golite062009
kinase activity	1.07563262976041	bayes_pls_golite062009
DNA binding	0.96398527572881	bayes_pls_golite062009
ligase activity	0.701001687425066	bayes_pls_golite062009
actin filament binding	0.58289829357591	bayes_pls_golite062009
structural molecule activity	0.537901980414372	bayes_pls_golite062009
molecular adaptor activity	0.494024302340603	bayes_pls_golite062009
SH3/SH2 adaptor activity	0.409062479526042	bayes_pls_golite062009
protein binding, bridging	0.370788151516215	bayes_pls_golite062009
transcription factor activity	0.256358801491972	bayes_pls_golite062009
signal transducer activity	0.150891693412241	bayes_pls_golite062009
molecular transducer activity	0.150891693412241	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2