Protein Overview: BSP1

General Information:
Name(s) found:	BSP1 / YPR171W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|786321\|gb\|AAB68105.1\| P9705.9 gene product [NCBI NR] gi\|74676417\|sp\|Q06604.1\|BSP1_YEAST RecName: Full=Protein BSP1; AltName: Full=Binding of synaptojanin polyphosphoinositide phosphatase domain protein 1 [NCBI NR] gi\|6325429\|ref\|NP_015497.1\| Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton; Bsp1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S59829 hypothetical protein YPR171w - yeast (Saccharomyces cerevisiae) [NCBI NR] Protein BSP1 OS=Saccharomyces cerevisiae GN=BSP1 PE=1 SV=1 [Swiss-Prot] BSP1 Protein involved in actin cytoskeleton organisation [mips] Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton [SGD] >Q06604 (Q06604) Chromosome XVI COSMID 9705 [4932.FASTACCERV.txt] Chr XVI from 883822-885552 [hmr_yeast5_DM3-1_pombe.fasta] Chr XVI from 883823-885553 [yeastorf3.fasta] YPR171W, Chr XVI from 883823-885553 [yeast_orfs2] BSP1 SGDID:S0006375, Chr XVI from 883822-885552, Verified ORF [yeastflysequest.fasta] BSP1 SGDID:S0006375, Chr XVI from 883824-885554, Verified ORF [10112005cervSGD.fasta] Binding protein of Synaptojanin Polyphosphoinositide phosphatase domain; may function to link synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton [Saccharomyces cerevisiae]gi\|2132312\|pir\|\|S59829 hypothetical protein YPR171w - yeast (Saccha [nrdb_11152004_Chlamydomonas] (Q06604) Ypr171wp [cerv4932.FASTAC] BSP1 SGDID:S000006375, Chr XVI from 883824-885554, Verified ORF, "Adapter that links synaptojanins Inp2p and Inp53p to the cortical actin cytoskeleton" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] BSP1 SGDID:S000006375, Chr XVI from 883824-885554, Verified ORF, "Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton" [yeast-contam.fasta] Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton; Bsp1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein BSP1 (Binding of synaptojanin polyphosphoinositide phosphatase domain protein 1) [nr-271106-contam.fasta] P9705.9 gene product [nr-271106-contam.fasta] [chemostatdb.fasta] Protein BSP1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BSP1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] BSP1 SGDID:S000006375, Chr XVI from 883828-885558, Genome Release 64-1-1, Verified ORF, "Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton" [yeast-030211-contam.fasta] BSP1 SGDID:S000006375, Chr XVI from 883828-885558, Genome Release 64-2-1, Verified ORF, "Adapter that links synaptojanins to the cortical actin cytoskeleton; the synaptojanins are Inp52p and Inp53p " [20141208_orf_trans.fasta] YPR171W SGDID:S000006375, Chr XVI from 883828-885558, Genome Release 64-1-1, Verified ORF, "Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	576 amino acids
Gene Ontology:
Cellular Component:	membrane fraction [IDA] cellular bud tip [IDA] cellular bud neck [IDA] actin cortical patch [IDA]
Biological Process:	actin cortical patch distribution [IMP]
Molecular Function:	protein binding, bridging [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTKYERDPEL VNFLSKVEDL NSKRYSNIPS SKPAGEALSP VRSHNSGEYR RADMMTGKNV  60
   61 EGCDNLAYRS AYNYEMTFSP KKTHYSLSEL NLERITPRPD LEGSASQKEK KFLISEEDYL 120
  121 LLQKLKASQT YNDSNADKNL PSFEKGPRMP SRGRPRPREK EIITIQYDFE LPGRADIPSS 180
  181 SSSSSPPPLP TRRDHIKITD GNEEKPLLPT RPNKAEVTES PSSRSIKPDA VVPERVKPAP 240
  241 PVSRSTKPAS FLSSLEDNKL TKAKSYNSEM ETPKTTVKSS HIDYLDSIQL KPTTLSPTMK 300
  301 NKPKPTPPSP PAKRIPRSES FIKSMLNSNL TTTSKPSLPE KPQKLRNANL AAHKTKPSIP 360
  361 PKKVELNIVL PELRPVETSP TKQNFENSID LPKLRSSNRN IKKEEEDSIP EAIKGIQNLK 420
  421 KTKQQKPAIP QKKSFLTNNS KNTTLKNGDD INKLNDEIEA LSLRNNLKKR PPTAPQRKIS 480
  481 LPEALRKVEL MKKSKTEPVL ESSNELSINA KLDAIIASRN LRASNTLPEL SGVNTNIATS 540
  541 DKYTTSRDET VKETKPLVHP NKNRTRGPRR KLPTRV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ABP1 BSP1 CMD1 CRN1 INP52 LSP1 MLC1 PRK1 RVS161 SLM1
View Details	Krogan NJ, et al. (2006)		BSP1 PTC7 SEC14 YKL091C
View Details	Gavin AC, et al. (2002)		BSP1 BZZ1 CHC1 ECM25 END3 INP52 LAS17 LSB3 RAD51 SLA1 SLA2 STM1 SYP1 TFP1 VRP1 YPT7 YSC84
View Details	Qiu et al. (2008)		ABP1 AIP1 BSP1 COF1 CRN1 END3 ENT1 HSK3 LAS17 MLC1 PAN1 SAC6 SCP1 SLA1 SLA2 TWF1 VRP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SLA1	BSP1	2	Drees BL, et al. (2001)
View Screen	GTS1	BSP1	2	Unpublished Fields Lab Data
View Screen	CAP1	BSP1	2	Drees BL, et al. (2001)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BSP1	Huh WK, et al. (2003)
View Data	BSP1 and SAC6	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..97]	deduced	N/A	No confident structure predictions are available.
2	View Details	[98..464]	MSA	12.260996	View MSA. No confident structure predictions are available.
3	View Details	[465..576]	MSA	1.230991	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2