Protein Overview: PLC1

General Information:
Name(s) found:	PLC1 / YPL268W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|7548846\|gb\|AAB27349.2\| delta class phosphoinositide-specific phospholipase C homolog [Saccharomyces cerevisiae] [NCBI NR] gi\|6324987\|ref\|NP_015055.1\| Phosphoinositide-specific phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in kinetochore function and pseudohyphal dif [NCBI NR] pir\|\|A47257 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase (EC 3.1.4.11) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|417490\|sp\|P32383.1\|PLC1_YEAST RecName: Full=1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase 1; AltName: Full=Phosphoinositide phospholipase C; AltName: Full=Phospholipase C-1; Short=PLC-1 [NCBI NR] gi\|287612\|dbj\|BAA02230.1\| phospholipase C [Saccharomyces cerevisiae] [NCBI NR] gi\|1370553\|emb\|CAA98004.1\| PLC1 [Saccharomyces cerevisiae] [NCBI NR] 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase 1 OS=Saccharomyces cerevisiae GN=PLC1 PE=1 SV=1 [Swiss-Prot] PLC1 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase [mips] Phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes [SGD] (P32383) 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase 1 (EC 3.1.4.11) (Phosphoinositide phospholipase C) (PLC-1) (Phospholipase C-1) [4932.FASTACCERV.txt] Chr XVI from 35236-37845 [hmr_yeast5_DM3-1_pombe.fasta] PLC1, Chr XVI from 35236-37845 [yeast_orfs2] PLC1 SGDID:S0006189, Chr XVI from 35236-37845, Verified ORF [yeastflysequest.fasta] Phosphoinositide-specific phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); affects kinetochore function possibly by modulating the structure of centromeric [nrdb_11152004_Chlamydomonas] PLC1 SGDID:S000006189, Chr XVI from 35236-37845, Verified ORF, "Phosphoinositide-specific phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in kinet [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PLC1 SGDID:S000006189, Chr XVI from 35236-37845, Verified ORF, "Phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulat [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Phosphoinositide-specific phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in kinetochore function and pseudohyphal differentiation; Plc1p [Sacchar [nr-271106-contam.fasta] PLC1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] phospholipase C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase 1 (Phosphoinositide phospholipase C) (PLC-1) (Phospholipase C-1) [nr-271106-contam.fasta] delta class phosphoinositide-specific phospholipase C homolog [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PLC1 SGDID:S000006189, Chr XVI from 35236-37845, Verified ORF, "Phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes" [yeast-bovrabprots.fasta] PLC1 SGDID:S000006189, Chr XVI from 35236-37845, Verified ORF, "Phosphoinositide-specific phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in kinetochore function and pseudohyphal differentiation" [yeast-contam_custom.fasta] [chemostatdb.fasta] 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PLC1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PLC1 SGDID:S000006189, Chr XVI from 35236-37845, Genome Release 64-1-1, Verified ORF, "Phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes" [yeast-030211-contam.fasta] PLC1 SGDID:S000006189, Chr XVI from 35236-37845, Genome Release 64-2-1, Verified ORF, "Phospholipase C; hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes; Plc1p and inositol polyphosphates are required for acetyl-CoA homeostasis which regulates global histone acetylation" [20141208_orf_trans.fasta] YPL268W SGDID:S000006189, Chr XVI from 35236-37845, Genome Release 64-1-1, Verified ORF, "Phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	869 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] condensed nuclear chromosome kinetochore [IDA]
Biological Process:	signal transduction [IMP] regulation of phosphate metabolic process [IMP] pseudohyphal growth [IMP] vacuole fusion, non-autophagic [IMP]
Molecular Function:	phosphoinositide phospholipase C activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTESAIDDQR FNLTKELQRH SCRDQGKITQ KDDALDFISY SSFQSSFNTD QKSANNGSTV  60
   61 RRSIRSIFRR AAELPRVHMG PLTYSHGINE LVNKKLRKDC DLSTLCRVLQ RGIRMIRMTR 120
  121 RRRKFYEFKL INNNGQIIWK DGSKYLELDS VKDIRIGDTA STYQEEVDPK RLRSDSKLWI 180
  181 AIIYKVSNKL KALHVVALNE LDFNTFLSCI CGLVKLRREL MESILLPDNS QFARIHWQIT 240
  241 VSEKEEDEKK DTLSFADVKK LCDKFHIYVS TGQLLEFFQL ADINHNGLLN YFEFEKFIKI 300
  301 LKNRKEVNMI WSKFTKPPHS HLSFENFFQF LITEQHEQVD RQTAWSYFIK YREPTQLTMG 360
  361 QDGFTKFLKE QPYLVEVKEE LYSKPLNHYF IASSHNTYLL GKQIAETPSV EGYIQVLQQG 420
  421 CRCVEIDIWD GENGPVVCHG FLTSAIPLKT VIRVIKKYAF ITSPYPLIIS LEINCNKDNQ 480
  481 KLASLIMREV LAEQLYFVGT RTDKLPSPRE LKHKILLKSK KTSEATRGLS VNEPFPSSFS 540
  541 SSYESANEQE LRMKDDSTNS SSATNSSSMQ RIKRIGLKKH ADIINDVSNI SGIHGIKFRN 600
  601 FSLPESKTIA HCFSLNERKV EYMIKDKHLK LSLDKHNRRY LMRVYPHVLR YKSSNFNPIP 660
  661 FWKAGVQMVA TNWQTNDIGQ QLNLAMFQIL DHQPDGSFKS GYVLKPKKLL PVVTKAKMIP 720
  721 LIYEHFENGS DPVTVKIRIL STQLLPRLND TSPSRNNTNS FVKVEFHTDD EPTMPISIDK 780
  781 GTRISATEAS TKSSQGNGFN PIWDAEVSIT LKDTDLTFIK FMVISEETQI ASVCLKLNYL 840
  841 RMGYRHIPLF NMEGEQYIFC TLFIHTQIL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BTT1 CAF130 EGD1 EGD2 PLC1 YJR011C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD51	PLC1	1	Unpublished Fields Lab Data
View Screen	UBC7	PLC1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..86]	deduced	N/A	No confident structure predictions are available.
2	View Details	[87..224]	PSI-BLAST	20.09691	Phospholipase C delta-1
3	View Details	[225..369]	PSI-BLAST	1576.9897	Phosphoinositide-specific phospholipase C, isozyme D1 (PLC-D!); PI-specific phospholipase C isozyme D1 (PLC-D1), C-terminal domain; Phospholipase C isozyme D1 (PLC-D1)
4	View Details	[370..726]	PSI-BLAST	1576.9897	Phosphoinositide-specific phospholipase C, isozyme D1 (PLC-D!); PI-specific phospholipase C isozyme D1 (PLC-D1), C-terminal domain; Phospholipase C isozyme D1 (PLC-D1)
5	View Details	[727..869]	PSI-BLAST	1576.9897	Phosphoinositide-specific phospholipase C, isozyme D1 (PLC-D!); PI-specific phospholipase C isozyme D1 (PLC-D1), C-terminal domain; Phospholipase C isozyme D1 (PLC-D1)

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
SH2 domain binding	3.65625799344693	bayes_pls_golite062009
binding	3.29080729281876	bayes_pls_golite062009
protein binding	3.17213698546013	bayes_pls_golite062009
phosphoinositide 3-kinase binding	2.88942227773914	bayes_pls_golite062009
insulin receptor binding	1.93662993549038	bayes_pls_golite062009
transcription regulator activity	1.57653457217606	bayes_pls_golite062009
nucleic acid binding	1.35724984140766	bayes_pls_golite062009
DNA binding	1.35019502849174	bayes_pls_golite062009
signal transducer activity	1.34134699517825	bayes_pls_golite062009
molecular transducer activity	1.34134699517825	bayes_pls_golite062009
transporter activity	1.28986800087234	bayes_pls_golite062009
cytoskeletal protein binding	1.20299294320291	bayes_pls_golite062009
enzyme binding	1.17212675145623	bayes_pls_golite062009
actin binding	1.15845374680034	bayes_pls_golite062009
receptor activity	1.02045773197699	bayes_pls_golite062009
transmembrane receptor activity	0.990048359575281	bayes_pls_golite062009
insulin-like growth factor receptor binding	0.893216890968741	bayes_pls_golite062009
substrate-specific transporter activity	0.79883446648737	bayes_pls_golite062009
receptor binding	0.57115269387131	bayes_pls_golite062009
protein domain specific binding	0.51731521446502	bayes_pls_golite062009
GTPase regulator activity	0.355387992614734	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	0.335498703113274	bayes_pls_golite062009
protein complex binding	0.22543440052247	bayes_pls_golite062009
enzyme regulator activity	0.189879136353541	bayes_pls_golite062009
Ran GTPase binding	0.0789278191015399	bayes_pls_golite062009
pyrophosphatase activity	0.0611639610381174	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.0524559849408587	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.0498422520006935	bayes_pls_golite062009

Term	Confidence	Notes
phosphoric diester hydrolase activity	4.7164034947025	bayes_pls_golite062009
glycerophosphodiester phosphodiesterase activity	4.27697417361287	bayes_pls_golite062009
phospholipase C activity	4.02608863873812	bayes_pls_golite062009
inositol or phosphatidylinositol phosphodiesterase activity	3.53845975637639	bayes_pls_golite062009
phosphoinositide phospholipase C activity	3.53845975637639	bayes_pls_golite062009
phospholipase activity	3.3535603060637	bayes_pls_golite062009
phosphoric ester hydrolase activity	3.27299307330343	bayes_pls_golite062009
ion transmembrane transporter activity	2.57032504567157	bayes_pls_golite062009
cation transmembrane transporter activity	2.55650624542181	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.37465721152046	bayes_pls_golite062009
metal ion transmembrane transporter activity	1.99763594451773	bayes_pls_golite062009
hydrolase activity	1.77704560318103	bayes_pls_golite062009
transcription regulator activity	1.70224798223084	bayes_pls_golite062009
binding	1.68488937304436	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	1.48964499825589	bayes_pls_golite062009
protein binding	1.44033231686342	bayes_pls_golite062009
nucleic acid binding	1.33654529506691	bayes_pls_golite062009
DNA binding	1.3098416020159	bayes_pls_golite062009
lipase activity	1.30779031242608	bayes_pls_golite062009
catalytic activity	1.22328099675039	bayes_pls_golite062009
cytoskeletal protein binding	1.21223335767967	bayes_pls_golite062009
transporter activity	1.15928534291452	bayes_pls_golite062009
molecular transducer activity	0.949608579595809	bayes_pls_golite062009
signal transducer activity	0.949608579595809	bayes_pls_golite062009
transmembrane transporter activity	0.945691505472547	bayes_pls_golite062009
cation channel activity	0.802117767859521	bayes_pls_golite062009
amine transmembrane transporter activity	0.717054504236607	bayes_pls_golite062009
substrate-specific transporter activity	0.693724160233023	bayes_pls_golite062009
motor activity	0.55768019172905	bayes_pls_golite062009
channel activity	0.386960491413065	bayes_pls_golite062009
passive transmembrane transporter activity	0.386960491413065	bayes_pls_golite062009
structural constituent of muscle	0.36302486156653	bayes_pls_golite062009
substrate-specific channel activity	0.362205423422174	bayes_pls_golite062009
actin binding	0.361058618811406	bayes_pls_golite062009
ion channel activity	0.343477643719352	bayes_pls_golite062009
voltage-gated cation channel activity	0.278054748078249	bayes_pls_golite062009
active transmembrane transporter activity	0.246383459363256	bayes_pls_golite062009
voltage-gated channel activity	0.203504070557827	bayes_pls_golite062009
potassium channel activity	0.200283164564768	bayes_pls_golite062009
voltage-gated ion channel activity	0.199755412261029	bayes_pls_golite062009
gated channel activity	0.150185445887481	bayes_pls_golite062009
voltage-gated potassium channel activity	0.109511951498555	bayes_pls_golite062009
structural molecule activity	0.0585855631213987	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2