Protein Overview: BCH1

General Information:
Name(s) found:	BCH1 / YMR237W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|887618\|emb\|CAA90208.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6323893\|ref\|NP_013964.1\| Member of the ChAPs family (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact wi [NCBI NR] gi\|2497198\|sp\|Q05029.1\|BCH1_YEAST RecName: Full=Protein BCH1; AltName: Full=BUD7 and CHS6 homolog 1 [NCBI NR] pir\|\|S57604 probable membrane protein YMR237w - yeast (Saccharomyces cerevisiae) [NCBI NR] (Q05029) Hypothetical 82.0 kDa protein in RNA1-RNT1 intergenic region [Swiss-Prot] Protein BCH1 OS=Saccharomyces cerevisiae GN=BCH1 PE=1 SV=1 [Swiss-Prot] BCH1 Protein involved in transport at the trans-Golgi [mips] Member of the ChAPs family (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes [SGD] YMR237W, Chr XIII from 743748-745922 [yeast_orfs2] Chr XIII from 743748-745922 [yeastorf3.fasta] YMR237W SGDID:S0004850, Chr XIII from 743748-745922, Uncharacterized ORF [yeastflysequest.fasta] BCH1 SGDID:S0004850, Chr XIII from 743748-745922, Uncharacterized ORF [10112005cervSGD.fasta] Bch1p [Saccharomyces cerevisiae]gi\|887618\|emb\|CAA90208.1\| unknown [Saccharomyces cerevisiae]gi\|2497198\|sp\|Q05029\|YM76_YEAST Hypothetical 82.0 kDa protein in RNA1-RNT1 intergenic regiongi\|1362401\|pir\|\|S57604 probable membrane protein YMR237w - yeast (Sa [nrdb_11152004_Chlamydomonas] YMR237W SGDID:S000004850, Chr XIII from 743748-745922, Uncharacterized ORF, "Protein that colocalizes with clathrin-coated vesicles; involved in transport at the trans-Golgi" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] BCH1 SGDID:S000004850, Chr XIII from 743748-745922, Verified ORF, "Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chiti [yeast-contam.fasta] BCH1 SGDID:S000004850, Chr XIII from 743748-745922, Verified ORF, "Member of the ChAPs family (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chitin synthase Chs3p, from the Golgi to plasma membrane; Bch1p [Sacchar [nr-271106-contam.fasta] Hypothetical 82.0 kDa protein in RNA1-RNT1 intergenic region [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] BCH1 SGDID:S000004850, Chr XIII from 743748-745922, Verified ORF, "Member of the ChAPs family (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes" [yeast-bovrabprots.fasta] BCH1 SGDID:S000004850, Chr XIII from 743748-745922, Verified ORF, "Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chitin synthase Chs3p, from the Golgi to plasma membrane" [yeast-contam_custom.fasta] [chemostatdb.fasta] Protein BCH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BCH1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] BCH1 SGDID:S000004850, Chr XIII from 743749-745923, Genome Release 64-1-1, Verified ORF, "Member of the ChAPs family (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes" [yeast-030211-contam.fasta] BCH1 SGDID:S000004850, Chr XIII from 743749-745923, Genome Release 64-2-1, Verified ORF, "Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YMR237W SGDID:S000004850, Chr XIII from 743749-745923, Genome Release 64-1-1, Verified ORF, "Member of the ChAPs family (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	724 amino acids
Gene Ontology:
Cellular Component:	ribosome [IDA] clathrin-coated vesicle [IDA]
Biological Process:	Golgi to plasma membrane transport [IGI] cell wall chitin biosynthetic process [IGI][IPI]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLSQTSIPEV KEDVIGYALH QRRARVGQFQ DLGPPDLITL IKSLPSSSST TTATASANDN  60
   61 GATSNINGQD PTTIVTELHS HDKLKGQIGT FFYCMGIDTS DPTSITIFAK KITDLFLDTP 120
  121 QIWFGKKKHF HVSKISISSW NAFRKYDVNI IVHIPGTVQT YIINSDGEQS QLPSVAEASS 180
  181 GRNSQDLNVN MIWAETFMSG IVRDIMIMKD NRADGESQNL VETLIFNPFT SGELEDVANN 240
  241 FIKLFPLVYE KGVYLDAPTH VLNPSLTNNY LVETLVEIVR LTKSLEACRK MLKKLIEIHP 300
  301 EAVIILIRVY FACDLEIDAV DLINEQLNSP SSFLADDSKT SHIQLIFKSE LLSIQSEFLL 360
  361 DVKRDYKLAK EVAMEAVNCA PNEFKTWYLL TRIYIKLNDM SNALLSLNAC PMSQVKEKYV 420
  421 LRRIAPITSD ENLHLPLPLD ASIEEISSLN PMDVQLEQKS ADPNLVNLSA SSLKSTFQLA 480
  481 YKLLTEIVQI TGWEQLLKYR SKIFVMEDEY QGSTSSIDEA EVRGNDISKM RSKRLCERWL 540
  541 DNLFMLLYED LKTYTDWQSE QLYFDAQNSK YHKLTVEWEL FGLCAKRLGH LPEAAKAFQI 600
  601 GLSQRFSPVC AKNLLQFYID EHKRIRRDSV SANSELTSSQ ILSSINDIDS SIIDLVVKIC 660
  661 CWNHRWYIEF SIILIDALSV AVQDMGITKV HNEIASRFSD PVAQLIDDNI LNFLKNFTND 720
  721 TFDN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BCH1 BCH2 CHS5
View Details	Krogan NJ, et al. (2006)		BCH1 BCH2 CHS5 LAP2 MAE1
View Details	Gavin AC, et al. (2006)		BCH1 BUD7 CHS5 CHS6
View Details	Qiu et al. (2008)		BCH1 BCH2 CHS6 ENT3 YOS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC28	BCH1	1	Unpublished Fields Lab Data
View Screen	LOS1	BCH1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	BCH1	Huh WK, et al. (2003)
View Data	BCH1 and CHC1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..415]	deduced	N/A	No confident structure predictions are available.
2	View Details	[416..724]	MSA	1.008926	View MSA. No confident structure predictions are available.
3	View Details	[637..724]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
drug binding	1.97908029685084	bayes_pls_golite062009
binding	1.52138759558575	bayes_pls_golite062009
transcription regulator activity	1.27878246750316	bayes_pls_golite062009
isomerase activity	1.19192091517095	bayes_pls_golite062009
catalytic activity	0.838457647933563	bayes_pls_golite062009
transporter activity	0.741927397485699	bayes_pls_golite062009
transmembrane transporter activity	0.489382802264305	bayes_pls_golite062009
substrate-specific transporter activity	0.18557772671331	bayes_pls_golite062009
peptidyl-prolyl cis-trans isomerase activity	0.161213797497172	bayes_pls_golite062009
protein binding	0.104527660115896	bayes_pls_golite062009
cis-trans isomerase activity	0.102982189630575	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2