Protein Overview: SWI6

General Information:
Name(s) found:	SWI6 / YLR182W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|RGBYW6 regulatory protein SWI6 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6323211\|ref\|NP_013283.1\| Transcription cofactor, forms complexes with DNA-binding proteins Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulated by phosphorylation; potential Cdc28 [NCBI NR] gi\|577200\|gb\|AAB67460.1\| Swi6p: Transcriptional regulatory protein [Saccharomyces cerevisiae] [NCBI NR] gi\|4598\|emb\|CAA29581.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|135078\|sp\|P09959.1\|SWI6_YEAST RecName: Full=Regulatory protein SWI6; AltName: Full=Cell-cycle box factor subunit SWI6; AltName: Full=Trans-acting activator of HO endonuclease gene; AltName: Full=MBF subunit P90 [NCBI NR] Regulatory protein SWI6 OS=Saccharomyces cerevisiae GN=SWI6 PE=1 SV=1 [Swiss-Prot] SWI6 Transcription factor [mips] Transcription cofactor, forms complexes with DNA-binding proteins Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulated by phosphorylation; potential Cdc28p substrate [SGD] SWI6, Chr XII from 517942-520353 [yeast_orfs2] Chr XII from 517942-520353 [yeastorf3.fasta] (P09959) Regulatory protein SWI6 (Cell-cycle box factor, chain SWI6) (Trans-acting activator of HO endonuclease gene) (MBF subunit P90) [4932.FASTACCERV.txt] SWI6 SGDID:S0004172, Chr XII from 517942-520353, Verified ORF [yeastflysequest.fasta] Swi6p [Saccharomyces cerevisiae]gi\|4598\|emb\|CAA29581.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|69001\|pir\|\|RGBYW6 regulatory protein SWI6 - yeast (Saccharomyces cerevisiae)gi\|577200\|gb\|AAB67460.1\| Swi6p: Transcriptional regulatory protein [nrdb_11152004_Chlamydomonas] SWI6 SGDID:S000004172, Chr XII from 517942-520353, Verified ORF, "Transcription cofactor, forms complexes with DNA-binding proteins Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulat [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Regulatory protein SWI6 (Cell-cycle box factor subunit SWI6) (Trans-acting activator of HO endonuclease gene) (MBF subunit P90) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Swi6p: Transcriptional regulatory protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Transcription cofactor, forms complexes with DNA-binding proteins Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulated by phosphorylation; potential Cdc28p substrate; Swi6p [Saccharo [nr-271106-contam.fasta] SWI6 SGDID:S000004172, Chr XII from 517942-520353, Verified ORF, "Transcription cofactor, forms complexes with DNA-binding proteins Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulated by phosphorylation; potential Cdc28p substrate" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Regulatory protein SWI6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SWI6 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SWI6 SGDID:S000004172, Chr XII from 517940-520351, Genome Release 64-1-1, Verified ORF, "Transcription cofactor, forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization" [yeast-030211-contam.fasta] SWI6 SGDID:S000004172, Chr XII from 517940-520351, Genome Release 64-2-1, Verified ORF, "Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators" [20141208_orf_trans.fasta] YLR182W SGDID:S000004172, Chr XII from 517940-520351, Genome Release 64-1-1, Verified ORF, "Transcription cofactor, forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	803 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA ] cytoplasm [IDA ]
Biological Process:	transcription [TAS] regulation of transcription involved in G1/S-phase of mitotic cell cycle [TAS] meiosis [IGI][IMP]
Molecular Function:	protein binding [IPI] transcription coactivator activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MALEEVVRYL GPHNEIPLTL TRDSETGHFL LKHFLPILQQ YHDTGNINET NPDSFPTDEE  60
   61 RNKLLAHYGI AVNTDDRGEL WIELEKCLQL LNMLNLFGLF QDAFEFEEPE TDQDEEDPSH 120
  121 SKLPENKTKS ENSKDNISSK RINNLQDMSL DSDAHRELGS PLKKLKIDTS VIDAESDSTP 180
  181 NTARGKPNDD INKGPSGDNE NNGTDDNDRT AGPIITFTHD LTSDFLSSPL KIMKALPSPV 240
  241 VNDNEQKMKL EAFLQRLLFP EIQEMPTSLN NDSSNRNSEG GSSNQQQQHV SFDSLLQEVN 300
  301 DAFPNTQLNL NIPVDEHGNT PLHWLTSIAN LELVKHLVKH GSNRLYGDNM GESCLVKAVK 360
  361 SVNNYDSGTF EALLDYLYPC LILEDSMNRT ILHHIIITSG MTGCSAAAKY YLDILMGWIV 420
  421 KKQNRPIQSG TNEKESKPND KNGERKDSIL ENLDLKWIIA NMLNAQDSNG DTCLNIAARL 480
  481 GNISIVDALL DYGADPFIAN KSGLRPVDFG AGTSKLQNTN GGDENSKMVS KGDYDGQKNG 540
  541 KAKKIRSQLL KNPPETTSLI NDVQNLLNSI SKDYENETVQ YNEKLEKLHK ELNEQREELA 600
  601 NSREQLANVK QLKDEYSLMQ EQLTNLKAGI EEEEESFREE SKKLGIIADE SSGIDWDSSE 660
  661 YDADEPFKVE FLSDFLEDKL QKNYEGDISK LLEAESKEQI MEQIRNQLPA EKIQSMLPPT 720
  721 VLLKARINAY KRNDKHLTNV LDTISTKQSE LENKFRRVLS LCLKIDENKV DNMLDGLLQA 780
  781 ISSEDPQDID TDEMQDFLKK HAS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		MBP1 SWI4 SWI6
View Details	(MIPS) Mewes HW, et al. (2004)		MBP1 SWI4 SWI6
View Details	Krogan NJ, et al. (2006)		BUD2 MBP1 MSA2 SWI4 SWI6 WHI5
View Details	Gavin AC, et al. (2006)		SWI4 SWI6
View Details	Gavin AC, et al. (2002)		MBP1 SWI6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	TOM22	SWI6	1	Unpublished Fields Lab Data
View Screen	COG4	SWI6	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SWI6	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..201]	MSA	1.285973	View MSA. No confident structure predictions are available.
2	View Details	[202..511]	PSI-BLAST	10043.0	Swi6 ankyrin-repeat fragment
3	View Details	[512..600]	PSI-BLAST	41.69897	bcl-3
4	View Details	[601..803]	ORFEUS	8.12	Prolactin (placental lactogen)

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
protein binding	2.69918165732603	bayes_pls_golite062009
binding	2.60916417202222	bayes_pls_golite062009
transcription regulator activity	2.37103377984038	bayes_pls_golite062009
nucleic acid binding	1.8957142857582	bayes_pls_golite062009
DNA binding	1.78305438715057	bayes_pls_golite062009
transcription activator activity	1.6418915400902	bayes_pls_golite062009
transcription factor activity	1.2667825651547	bayes_pls_golite062009
transcription factor binding	1.06366405671195	bayes_pls_golite062009
RNA binding	0.969928411729387	bayes_pls_golite062009
sequence-specific DNA binding	0.949906091645366	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.590503097160372	bayes_pls_golite062009
cyclin-dependent protein kinase regulator activity	0.0462605323512499	bayes_pls_golite062009

Term	Confidence	Notes
protein binding	2.69918165732603	bayes_pls_golite062009
binding	2.60916417202222	bayes_pls_golite062009
transcription regulator activity	2.37103377984038	bayes_pls_golite062009
nucleic acid binding	1.8957142857582	bayes_pls_golite062009
DNA binding	1.78305438715057	bayes_pls_golite062009
transcription activator activity	1.6418915400902	bayes_pls_golite062009
transcription factor activity	1.2667825651547	bayes_pls_golite062009
transcription factor binding	1.06366405671195	bayes_pls_golite062009
RNA binding	0.969928411729387	bayes_pls_golite062009
sequence-specific DNA binding	0.949906091645366	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.590503097160372	bayes_pls_golite062009
cyclin-dependent protein kinase regulator activity	0.0462605323512499	bayes_pls_golite062009

Term	Confidence	Notes
protein binding	3.32153200117175	bayes_pls_golite062009
binding	3.246345109977	bayes_pls_golite062009
receptor binding	2.75738439909781	bayes_pls_golite062009
transcription regulator activity	2.27089567891838	bayes_pls_golite062009
cytokine receptor binding	1.13209989837612	bayes_pls_golite062009
cytokine activity	0.865612008604712	bayes_pls_golite062009
growth factor activity	0.805183150393473	bayes_pls_golite062009
catalytic activity	0.263255675478865	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2