Protein Overview: ERG27

General Information:
Name(s) found:	ERG27 / YLR100W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323129\|ref\|NP_013201.1\| 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs; Erg27p [Saccharomyces cerevisiae] [NCBI NR] gi\|60392263\|sp\|Q12452.1\|ERG27_YEAST RecName: Full=3-keto-steroid reductase [NCBI NR] pir\|\|S64936 probable membrane protein YLR100w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1360483\|emb\|CAA97664.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1256850\|gb\|AAB67544.1\| Ylr100wp [Saccharomyces cerevisiae] [NCBI NR] 3-keto-steroid reductase OS=Saccharomyces cerevisiae GN=ERG27 PE=1 SV=1 [Swiss-Prot] ERG27 3-keto sterol reductase [mips] 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs [SGD] ERG27, Chr XII from 341811-342854 [yeast_orfs2] Chr XII from 341811-342854 [yeastorf3.fasta] >Q12452 (Q12452) ORF YLR100W [4932.FASTACCERV.txt] ERG27 SGDID:S0004090, Chr XII from 341811-342854, Verified ORF [yeastflysequest.fasta] 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs [Saccharomyces cerevisiae]gi\|1360483\|emb\|CAA97664.1\| unnamed protein produc [nrdb_11152004_Chlamydomonas] (Q12452) ORF YLR100w (Ylr100wp) [cerv4932.FASTAC] ERG27 SGDID:S000004090, Chr XII from 341811-342854, Verified ORF, "3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Ylr100wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 3-keto-steroid reductase [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs; Erg27p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] 3-keto-steroid reductase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERG27 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ERG27 SGDID:S000004090, Chr XII from 341810-342853, Genome Release 64-1-1, Verified ORF, "3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs" [yeast-030211-contam.fasta] ERG27 SGDID:S000004090, Chr XII from 341810-342853, Genome Release 64-2-1, Verified ORF, "3-keto sterol reductase; catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs" [20141208_orf_trans.fasta] YLR100W SGDID:S000004090, Chr XII from 341810-342853, Genome Release 64-1-1, Verified ORF, "3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	347 amino acids
Gene Ontology:
Cellular Component:	endoplasmic reticulum membrane [IDA] endoplasmic reticulum [IDA] mitochondrial outer membrane [IDA]
Biological Process:	ergosterol biosynthetic process [IMP]
Molecular Function:	3-keto sterol reductase activity [IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNRKVAIVTG TNSNLGLNIV FRLIETEDTN VRLTIVVTSR TLPRVQEVIN QIKDFYNKSG  60
   61 RVEDLEIDFD YLLVDFTNMV SVLNAYYDIN KKYRAINYLF VNAAQGIFDG IDWIGAVKEV 120
  121 FTNPLEAVTN PTYKIQLVGV KSKDDMGLIF QANVFGPYYF ISKILPQLTR GKAYIVWISS 180
  181 IMSDPKYLSL NDIELLKTNA SYEGSKRLVD LLHLATYKDL KKLGINQYVV QPGIFTSHSF 240
  241 SEYLNFFTYF GMLCLFYLAR LLGSPWHNID GYKAANAPVY VTRLANPNFE KQDVKYGSAT 300
  301 SRDGMPYIKT QEIDPTGMSD VFAYIQKKKL EWDEKLKDQI VETRTPI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		ARG4 AYR1 COR1 CPR6 ERG27 GPT2 LIA1 MAE1 OM45 PCT1 PDA1 PET10 PET9 PIL1 PRE10 PRO3 RET2 RNR2 RTN2 RVS161 SEC18 SEC21 SEC26 SEC27 SEC28 TIF34 TIF35 TPA1 YNL181W
View Details	Qiu et al. (2008)		CYB5 ERG27

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC53	ERG27	2	Uetz P, et al. (2000)
View Screen	ERG27	PDR3	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	ERG27	Huh WK, et al. (2003)
View Data	ERG27 and ERG6	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..347]	PSI-BLAST	64.69897	Glucose dehydrogenase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
steroid dehydrogenase activity	3.70115036051018	bayes_pls_golite062009
steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	3.56748749904419	bayes_pls_golite062009
oxidoreductase activity, acting on CH-OH group of donors	2.27417613545298	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	1.97774592602866	bayes_pls_golite062009
catalytic activity	1.79741889688267	bayes_pls_golite062009
C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity	1.03845584151228	bayes_pls_golite062009
3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity	0.744959961067911	bayes_pls_golite062009
signal transducer activity	0.620476349006324	bayes_pls_golite062009
molecular transducer activity	0.620476349006324	bayes_pls_golite062009
binding	0.553666136934364	bayes_pls_golite062009
retinol dehydrogenase activity	0.48259320361048	bayes_pls_golite062009
oxidoreductase activity	0.39101464838432	bayes_pls_golite062009
receptor activity	0.0485610523889735	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2