Protein Overview: DMA1

General Information:
Name(s) found:	DMA1 / YHR115C [SGD]
Description(s) found: Found 28 descriptions. SHOW ALL	Protein involved in ubiquitin ligation; plays a role in regulating spindle position and orientation; functionally redundant with Dma2p; orthologous to human RNF8 protein, also has sequence similarity to human Chfr. [SGD]
Organism:	Saccharomyces cerevisiae
Length:	416 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	mitotic cell cycle spindle assembly checkpoint [IGI] establishment of mitotic spindle orientation [IGI] septin ring assembly [IGI]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTNTVPSSP PNQTPPAASG IATSHDHTKF NNPIRLPISI SLTINDTPNN NSNNNSVSNG  60
   61 LGILPSRTAT SLVVANNGSA NGNVGATAAA AATVETNTAP AVNTTKSIRH FIYPPNQVNQ 120
  121 TEFSLDIHLP PNTSLPERID QSTLKRRMDK HGLFSIRLTP FIDTSSTSVA NQGLFFDPII 180
  181 RTAGAGSQII IGRYTERVRE AISKIPDQYH PVVFKSKVIS RTHGCFKVDD QGNWFLKDVK 240
  241 SSSGTFLNHQ RLSSASTTSK DYLLHDGDII QLGMDFRGGT EEIYRCVKMK IELNKSWKLK 300
  301 ANAFNKEALS RIKNLQKLTT GLEQEDCSIC LNKIKPCQAI FISPCAHSWH FHCVRRLVIM 360
  361 NYPQFMCPNC RTNCDLETTL ESESESEFEN EDEDEPDIEM DIDMEINNNL GVRLVD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		DMA1 DMA2 ERG1 RPT2 RPT4 RVB1 SKP1 STI1 UBI4 YNL311C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	DMA1	YEF1	2	Uetz P, et al. (2000)
View Screen	DMA1	CDC123	2	Uetz P, et al. (2000)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	DMA1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..118]	deduced	N/A	No confident structure predictions are available.
2	View Details	[119..326] [380..416]	PSI-BLAST	6.0	Cbl; N-terminal domain of cbl (N-cbl); CBL
3	View Details	[327..379]	PSI-BLAST	6.0	Cbl; N-terminal domain of cbl (N-cbl); CBL

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
cytoskeletal protein binding	3.49668552859347	bayes_pls_golite062009
motor activity	3.25814691068414	bayes_pls_golite062009
binding	2.69791297400248	bayes_pls_golite062009
tubulin binding	2.51210014078446	bayes_pls_golite062009
actin binding	2.38676888633305	bayes_pls_golite062009
ubiquitin-protein ligase activity	2.20438586677555	bayes_pls_golite062009
microtubule binding	2.17136406283012	bayes_pls_golite062009
protein binding	2.14586566310919	bayes_pls_golite062009
small conjugating protein ligase activity	1.97264874084155	bayes_pls_golite062009
molecular adaptor activity	1.33740973673697	bayes_pls_golite062009
structural constituent of muscle	1.30669499755555	bayes_pls_golite062009
acid-amino acid ligase activity	1.26357595913158	bayes_pls_golite062009
SH3/SH2 adaptor activity	1.2323381312171	bayes_pls_golite062009
microfilament motor activity	1.0725773039399	bayes_pls_golite062009
signal transducer activity	0.999269271132931	bayes_pls_golite062009
molecular transducer activity	0.999269271132931	bayes_pls_golite062009
protein binding, bridging	0.83823250804321	bayes_pls_golite062009
actin filament binding	0.836375875773328	bayes_pls_golite062009
structural molecule activity	0.800850121197479	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.748479359639348	bayes_pls_golite062009
kinase activity	0.662911740280035	bayes_pls_golite062009
N-terminal myristoylation domain binding	0.63045154489014	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.493362981045534	bayes_pls_golite062009
G-protein beta/gamma-subunit binding	0.487272307313601	bayes_pls_golite062009
enzyme regulator activity	0.456661966897198	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.452350351194157	bayes_pls_golite062009
protein phosphorylated amino acid binding	0.43808715710519	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.382055332967642	bayes_pls_golite062009
enzyme activator activity	0.369738999181035	bayes_pls_golite062009
actin-dependent ATPase activity	0.3688716707761	bayes_pls_golite062009
pyrophosphatase activity	0.321819287555157	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.30564870400158	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.305174269756747	bayes_pls_golite062009
kinase regulator activity	0.262406945568467	bayes_pls_golite062009
transferase activity	0.169970258787201	bayes_pls_golite062009
phosphoprotein binding	0.16798254471727	bayes_pls_golite062009
myosin binding	0.125186564742711	bayes_pls_golite062009
structural constituent of cytoskeleton	0.116648335890738	bayes_pls_golite062009
protein kinase activity	0.0865488336042732	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2