Protein Overview: RAD54

General Information:
Name(s) found:	RAD54 / YGL163C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|JH0440 RAD54 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|728692\|emb\|CAA88534.1\| RAD54 [Saccharomyces cerevisiae] [NCBI NR] gi\|6321275\|ref\|NP_011352.1\| DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SN [NCBI NR] gi\|417577\|sp\|P32863.1\|RAD54_YEAST RecName: Full=DNA repair and recombination protein RAD54 [NCBI NR] gi\|172343\|gb\|AAA34949.1\| recombination and repair protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1322760\|emb\|CAA96875.1\| RAD54 [Saccharomyces cerevisiae] [NCBI NR] DNA repair and recombination protein RAD54 OS=Saccharomyces cerevisiae GN=RAD54 PE=1 SV=1 [Swiss-Prot] (P32863) DNA repair and recombination protein RAD54 (EC 3.6.1.-) [Swiss-Prot] RAD54 DNA-dependent ATPase of the Snf2p family [mips] DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family [SGD] RAD54, Chr VII from 193711-196407, reverse complement [yeast_orfs2] Chr VII from 193711-196407, reverse complement [yeastorf3.fasta] Chr VII from 196407-193711, reverse complement [yeastorf5.fasta] (P32863) DNA repair and recombination protein RAD54 [4932.FASTACCERV.txt] RAD54 SGDID:S0003131, Chr VII from 196407-193711, reverse complement, Verified ORF [yeastflysequest.fasta] RAD54 SGDID:S0003131, Chr VII from 196409-193713, reverse complement, Verified ORF [10112005cervSGD.fasta] DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family [Saccharomyces cere [nrdb_11152004_Chlamydomonas] RAD54 SGDID:S000003131, Chr VII from 196409-193713, reverse complement, Verified ORF, "DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] DNA repair and recombination protein RAD54 [nr-271106-contam.fasta] DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; Rad54p [Saccharomy [nr-271106-contam.fasta] RAD54 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] recombination and repair protein [nr-271106-contam.fasta] RAD54 SGDID:S000003131, Chr VII from 196409-193713, reverse complement, Verified ORF, "DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family" [yeast-bovrabprots.fasta] [chemostatdb.fasta] DNA repair and recombination protein RAD54 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RAD54 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RAD54 SGDID:S000003131, Chr VII from 196403-193707, Genome Release 64-1-1, reverse complement, Verified ORF, "DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family" [yeast-030211-contam.fasta] RAD54 SGDID:S000003131, Chr VII from 196403-193707, Genome Release 64-2-1, reverse complement, Verified ORF, "DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress" [20141208_orf_trans.fasta] YGL163C SGDID:S000003131, Chr VII from 196403-193707, Genome Release 64-1-1, reverse complement, Verified ORF, "DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	898 amino acids
Gene Ontology:
Cellular Component:	nucleus [IMP]
Biological Process:	chromatin remodeling [IDA] heteroduplex formation [IDA] [NOT]double-strand break repair via single-strand annealing [IMP] telomere maintenance via recombination [IMP] double-strand break repair via synthesis-dependent strand annealing [TAS]
Molecular Function:	DNA-dependent ATPase activity [IDA] DNA topoisomerase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MARRRLPDRP PNGIGAGERP RLVPRPINVQ DSVNRLTKPF RVPYKNTHIP PAAGRIATGS  60
   61 DNIVGGRSLR KRSATVCYSG LDINADEAEY NSQDISFSQL TKRRKDALSA QRLAKDPTRL 120
  121 SHIQYTLRRS FTVPIKGYVQ RHSLPLTLGM KKKITPEPRP LHDPTDEFAI VLYDPSVDGE 180
  181 MIVHDTSMDN KEEESKKMIK STQEKDNINK EKNSQEERPT QRIGRHPALM TNGVRNKPLR 240
  241 ELLGDSENSA ENKKKFASVP VVIDPKLAKI LRPHQVEGVR FLYRCVTGLV MKDYLEAEAF 300
  301 NTSSEDPLKS DEKALTESQK TEQNNRGAYG CIMADEMGLG KTLQCIALMW TLLRQGPQGK 360
  361 RLIDKCIIVC PSSLVNNWAN ELIKWLGPNT LTPLAVDGKK SSMGGGNTTV SQAIHAWAQA 420
  421 QGRNIVKPVL IISYETLRRN VDQLKNCNVG LMLADEGHRL KNGDSLTFTA LDSISCPRRV 480
  481 ILSGTPIQND LSEYFALLSF SNPGLLGSRA EFRKNFENPI LRGRDADATD KEITKGEAQL 540
  541 QKLSTIVSKF IIRRTNDILA KYLPCKYEHV IFVNLKPLQN ELYNKLIKSR EVKKVVKGVG 600
  601 GSQPLRAIGI LKKLCNHPNL LNFEDEFDDE DDLELPDDYN MPGSKARDVQ TKYSAKFSIL 660
  661 ERFLHKIKTE SDDKIVLISN YTQTLDLIEK MCRYKHYSAV RLDGTMSINK RQKLVDRFND 720
  721 PEGQEFIFLL SSKAGGCGIN LIGANRLILM DPDWNPAADQ QALARVWRDG QKKDCFIYRF 780
  781 ISTGTIEEKI FQRQSMKMSL SSCVVDAKED VERLFSSDNL RQLFQKNENT ICETHETYHC 840
  841 KRCNAQGKQL KRAPAMLYGD ATTWNHLNHD ALEKTNDHLL KNEHHYNDIS FAFQYISH

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		RAD51 RAD52 RAD54 RAD55 RAD57
View Details	Ho Y, et al. (2002)		MDH1 MGE1 RAD54 TMA19
View Details	Qiu et al. (2008)		RAD51 RAD54 RAD55 RAD57

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	RAD54	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
transcription repressor activity	3.46705970365511	bayes_pls_golite062009
transcription regulator activity	3.2394894333307	bayes_pls_golite062009
binding	3.06758476214113	bayes_pls_golite062009
chromatin binding	2.9265077396229	bayes_pls_golite062009
histone binding	2.00388175060339	bayes_pls_golite062009
nucleic acid binding	1.7870619298179	bayes_pls_golite062009
DNA binding	1.72304410722296	bayes_pls_golite062009
protein binding	1.61683263008775	bayes_pls_golite062009
methylated histone residue binding	1.03331560428208	bayes_pls_golite062009
general transcriptional repressor activity	0.49996033797134	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.356338632175355	bayes_pls_golite062009
satellite DNA binding	0.327386416436959	bayes_pls_golite062009
pyrophosphatase activity	0.309377712814897	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.296839540568239	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.29636000593974	bayes_pls_golite062009
catalytic activity	0.14067200158938	bayes_pls_golite062009

Term	Confidence	Notes
microtubule motor activity	3.4297361345158	bayes_pls_golite062009
nucleoside-triphosphatase activity	3.0989886302218	bayes_pls_golite062009
pyrophosphatase activity	3.01886747063507	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.99763530427224	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.99650240285889	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
motor activity	2.60481158518321	bayes_pls_golite062009
nucleic acid binding	2.11222585187695	bayes_pls_golite062009
DNA binding	1.7904783386531	bayes_pls_golite062009
hydrolase activity	1.77231681433173	bayes_pls_golite062009
ATPase activity	1.76238574289941	bayes_pls_golite062009
transcription regulator activity	1.68903321260301	bayes_pls_golite062009
ATPase activity, coupled	1.57826779898847	bayes_pls_golite062009
cytoskeletal protein binding	1.50574672650697	bayes_pls_golite062009
structural constituent of ribosome	1.47858140861683	bayes_pls_golite062009
microfilament motor activity	1.28029120876672	bayes_pls_golite062009
DNA-dependent ATPase activity	1.2155186842031	bayes_pls_golite062009
helicase activity	1.16085745407703	bayes_pls_golite062009
tubulin binding	1.01673904002272	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
microtubule binding	0.902404986889887	bayes_pls_golite062009
actin binding	0.875514430441177	bayes_pls_golite062009
structural molecule activity	0.86514677273731	bayes_pls_golite062009
plus-end-directed microtubule motor activity	0.773586048423911	bayes_pls_golite062009
DNA helicase activity	0.72881469450553	bayes_pls_golite062009
purine nucleotide binding	0.725611289205392	bayes_pls_golite062009
structure-specific DNA binding	0.720649395550746	bayes_pls_golite062009
nucleotide binding	0.717697610309727	bayes_pls_golite062009
purine ribonucleotide binding	0.713814218071151	bayes_pls_golite062009
ribonucleotide binding	0.713752628021599	bayes_pls_golite062009
transcription factor activity	0.603758349693077	bayes_pls_golite062009
purine NTP-dependent helicase activity	0.59513742813869	bayes_pls_golite062009
ATP-dependent helicase activity	0.59513742813869	bayes_pls_golite062009
double-stranded DNA binding	0.58862275163252	bayes_pls_golite062009
structural constituent of muscle	0.559419947521782	bayes_pls_golite062009
telomeric DNA binding	0.40354211833657	bayes_pls_golite062009
transcription repressor activity	0.244736544804496	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.22150712609541	bayes_pls_golite062009
ATP binding	0.19169045543599	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
adenyl ribonucleotide binding	0.165732470448462	bayes_pls_golite062009
adenyl nucleotide binding	0.162060759063149	bayes_pls_golite062009
substrate-specific transporter activity	0.144154288308693	bayes_pls_golite062009
active transmembrane transporter activity	0.114463153989353	bayes_pls_golite062009
GTPase activity	0.105546189348878	bayes_pls_golite062009
guanyl nucleotide binding	0.0289841199143713	bayes_pls_golite062009
translation regulator activity	0.0269721463618237	bayes_pls_golite062009
guanyl ribonucleotide binding	0.0187537754688351	bayes_pls_golite062009
GTP binding	0.0171298013679982	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.0028386101905622	bayes_pls_golite062009

No functions predicted.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..223]	MSA	1.238994	View MSA. No confident structure predictions are available.
2	View Details	[224..275]	ORFEUS	13.09	RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
3	View Details	[276..583]	ORFEUS	13.09	RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
4	View Details	[584..640]	PSI-BLAST	8.221849	Putative DEAD box RNA helicase
5	View Details	[641..898]	PSI-BLAST	7.045757	Nucleotide excision repair enzyme UvrB

Protein Overview: RAD54

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: