Protein Overview: CWH41

General Information:
Name(s) found:	CWH41 / YGL027C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321411\|ref\|NP_011488.1\| Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing [NCBI NR] pir\|\|S62136 CWH41 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1706208\|sp\|P53008.1\|CWH41_YEAST RecName: Full=Mannosyl-oligosaccharide glucosidase; AltName: Full=Processing A-glucosidase I; Short=Glucosidase I [NCBI NR] gi\|1322498\|emb\|CAA96728.1\| CWH41 [Saccharomyces cerevisiae] [NCBI NR] gi\|1176413\|gb\|AAC49157.1\| Cwh41p [NCBI NR] Mannosyl-oligosaccharide glucosidase OS=Saccharomyces cerevisiae GN=CWH41 PE=1 SV=1 [Swiss-Prot] (P53008) Mannosyl-oligosaccharide glucosidase (EC 3.2.1.106) (Processing A-glucosidase I) (Glucosidase I) [Swiss-Prot] CWH41 ER glucosidase I [mips] Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress [SGD] CWH41, Chr VII from 443638-446139, reverse complement [yeast_orfs2] Chr VII from 443638-446139, reverse complement [yeastorf3.fasta] Chr VII from 443645-446146, reverse complement [yeastorf4.fasta] Chr VII from 446146-443645, reverse complement [yeastorf5.fasta] CWH41 SGDID:S0002995, Chr VII from 446146-443645, reverse complement, Verified ORF [yeastflysequest.fasta] CWH41 SGDID:S0002995, Chr VII from 446148-443647, reverse complement, Verified ORF [10112005cervSGD.fasta] Cwh41p [Saccharomyces cerevisiae]gi\|1322498\|emb\|CAA96728.1\| CWH41 [Saccharomyces cerevisiae]gi\|1176413\|gb\|AAC49157.1\| Cwh41pgi\|2131218\|pir\|\|S62136 CWH41 protein - yeast (Saccharomyces cerevisiae)gi\|1706208\|sp\|P53008\|CW41_YEAST Mannosyl-oligosaccharide [nrdb_11152004_Chlamydomonas] CWH41 SGDID:S000002995, Chr VII from 446148-443647, reverse complement, Verified ORF, "Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylatio [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress; Cwh41p [Saccha [nr-271106-contam.fasta] Cwh41p [nr-271106-contam.fasta] CWH41 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mannosyl-oligosaccharide glucosidase (Processing A-glucosidase I) (Glucosidase I) [nr-271106-contam.fasta] CWH41 SGDID:S000002995, Chr VII from 446148-443647, reverse complement, Verified ORF, "Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Mannosyl-oligosaccharide glucosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CWH41 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CWH41 SGDID:S000002995, Chr VII from 446143-443642, Genome Release 64-1-1, reverse complement, Verified ORF, "Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress" [yeast-030211-contam.fasta] CWH41 SGDID:S000002995, Chr VII from 446143-443642, Genome Release 64-2-1, reverse complement, Verified ORF, "Processing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress" [20141208_orf_trans.fasta] YGL027C SGDID:S000002995, Chr VII from 446143-443642, Genome Release 64-1-1, reverse complement, Verified ORF, "Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	833 amino acids
Gene Ontology:
Cellular Component:	endoplasmic reticulum membrane [IDA]
Biological Process:	cellular cell wall organization [IMP][ISS] protein amino acid N-linked glycosylation [IMP]
Molecular Function:	mannosyl-oligosaccharide glucosidase activity [IDA][IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLISKSKMFK TFWILTSIVL LASATVDISK LQEFEEYQKF TNESLLWAPY RSNCYFGMRP  60
   61 RYVHESPLIM GIMWFNSLSQ DGLHSLRHFA TPQDKLQKYG WEVYDPRIGG KEVFIDEKNN 120
  121 LNLTVYFVKS KNGENWSVRV QGEPLDPKRP STASVVLYFS QNGGEIDGKS SLAMIGHDGP 180
  181 NDMKFFGYSK ELGEYHLTVK DNFGHYFKNP EYETMEVAPG SDCSKTSHLS LQIPDKEVWK 240
  241 ARDVFQSLVS DSIRDILEKE ETKQRPADLI PSVLTIRNLY NFNPGNFHYI QKTFDLTKKD 300
  301 GFQFDITYNK LGTTQSISTR EQVTELITWS LNEINARFDK QFSFGEGPDS IESVEVKRRF 360
  361 ALETLSNLLG GIGYFYGNQL IDRETEFDES QFTEIKLLNA KEEGPFELFT SVPSRGFFPR 420
  421 GFYWDEGFHL LQIMEYDFDL AFEILASWFE MIEDDSGWIA REIILGNEAR SKVPQEFQVQ 480
  481 NPNIANPPTL LLAFSEMLSR AIENIGDFNS DSYHQVMFNS RTAKFMTNNL EANPGLLTEY 540
  541 AKKIYPKLLK HYNWFRKSQT GLIDEYEEIL EDEGIWDKIH KNEVYRWVGR TFTHCLPSGM 600
  601 DDYPRAQPPD VAELNVDALA WVGVMTRSMK QIAHVLKLTQ DEQRYAQIEQ EVVENLDLLH 660
  661 WSENDNCYCD ISIDPEDDEI REFVCHEGYV SVLPFALKLI PKNSPKLEKV VALMSDPEKI 720
  721 FSDYGLLSLS RQDDYFGKDE NYWRGPIWMN INYLCLDAMR YYYPEVILDV AGEASNAKKL 780
  781 YQSLKINLSN NIYKVWEEQG YCYENYSPID GHGTGAEHFT GWTALVVNIL GRF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ADE2 BSC2 CWH41 DBF4 DIG1 ECM29 HAL5 LAA1 MRC1 OPI9 PEX32 PRP5 PTH2 RGA2 RMA1 RPN3 SIR1 STE12 THR1 TLG2 VPS45 YDL199C YEL057C YPL035C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	LIA1	CWH41	1	Unpublished Fields Lab Data
View Screen	LOS1	CWH41	1	Unpublished Fields Lab Data
View Screen	YLL037W	CWH41	1	Hazbun TR, et al. (2003)
View Screen	YPT53	CWH41	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..833]	Pfam	1000.0	Mannosyl oligosaccharide glucosidase No confident structure predictions are available.
2	View Details	[182..275]	deduced	N/A	No confident structure predictions are available.
3	View Details	[276..833]	PSI-BLAST	101.0	No description for 2jf4A was found.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
hydrolase activity, hydrolyzing O-glycosyl compounds	3.49882811932206	bayes_pls_golite062009
hydrolase activity, acting on glycosyl bonds	3.13521240810366	bayes_pls_golite062009
catalytic activity	1.16285856935495	bayes_pls_golite062009
binding	1.06330183651166	bayes_pls_golite062009
trehalase activity	0.389375574251869	bayes_pls_golite062009
protein binding	0.18984948020481	bayes_pls_golite062009
alpha,alpha-trehalase activity	0.17719034423607	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2