Abstract
CWH41 encodes a novel type II integral membrane N-glycoprotein located in the endoplasmic reticulum. Disruption of the CWH41 gene leads to a K1 killer toxin-resistant phenotype and a 50% reduction in the cell wall beta 1,6-glucan level. CWH41 also displays strong genetic interactions with KRE1 and KRE6, two genes known to be involved in the beta 1,6-glucan biosynthetic pathway. The cwh41 delta kre6 delta double mutant is nonviable; and the cwh41 delta kre1 delta double mutation results in strong synergistic defects, with a severely slow-growth phenotype, a 75% reduction in beta 1,6-glucan level, and the secretion of a cell wall glucomannoprotein, Cwp1p. These results provide strong genetic evidence indicating that Cwh41p plays a functional role, possibly as a new synthetic component, in the assembly of cell wall beta 1,6-glucan.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Western
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Cell Compartmentation
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Cell Wall / metabolism*
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Cloning, Molecular
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Crosses, Genetic
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Drug Resistance, Microbial
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Endoplasmic Reticulum / chemistry*
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Fungal Proteins / analysis
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Fungal Proteins / genetics*
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Genes, Fungal*
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Glucans / metabolism*
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Glycoproteins / metabolism
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Killer Factors, Yeast
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Membrane Glycoproteins / analysis
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Membrane Glycoproteins / genetics*
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Membrane Glycoproteins / immunology
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Membrane Proteins / genetics
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Molecular Sequence Data
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Mutation
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Mycotoxins / pharmacology
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Phenotype
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis, DNA
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alpha-Glucosidases
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beta-Glucans*
Substances
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Fungal Proteins
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Glucans
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Glycoproteins
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K1 killer toxin
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KRE1 protein, S cerevisiae
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KRE6 protein, S cerevisiae
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Killer Factors, Yeast
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Membrane Glycoproteins
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Membrane Proteins
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Mycotoxins
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Saccharomyces cerevisiae Proteins
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beta-Glucans
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beta-1,6-glucan
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CWH41 protein, S cerevisiae
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alpha-Glucosidases