Protein Overview: PEA2

General Information:
Name(s) found:	PEA2 / YER149C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|731525\|sp\|P40091.1\|PEA2_YEAST RecName: Full=Protein PEA2; AltName: Full=Protein PPF2 [NCBI NR] gi\|6320997\|ref\|NP_011076.1\| Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth; Pea2p [Saccharomyces [NCBI NR] gi\|603389\|gb\|AAB64676.1\| Pea2p [Saccharomyces cerevisiae] [NCBI NR] gi\|45719876\|emb\|CAE52156.1\| Pea2p [Saccharomyces cerevisiae] [NCBI NR] gi\|45719874\|emb\|CAE52155.1\| Pea2p [Saccharomyces cerevisiae] [NCBI NR] gi\|45719872\|emb\|CAE52154.1\| Pea2p [Saccharomyces cerevisiae] [NCBI NR] gi\|45719864\|emb\|CAE52150.1\| Pea2p [Saccharomyces cerevisiae] [NCBI NR] gi\|45719860\|emb\|CAE52148.1\| Pea2p [Saccharomyces cerevisiae] [NCBI NR] gi\|45719852\|emb\|CAE52144.1\| Pea2p [Saccharomyces cerevisiae] [NCBI NR] gi\|45719846\|emb\|CAE52141.1\| Pea2p [Saccharomyces cerevisiae] [NCBI NR] gi\|1752720\|dbj\|BAA07465.1\| Ppf2p [Saccharomyces cerevisiae] [NCBI NR] gi\|1502362\|emb\|CAA68869.1\| Pea2 protein [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S50652 hypothetical protein YER149c - yeast (Saccharomyces cerevisiae) [NCBI NR] Protein PEA2 OS=Saccharomyces cerevisiae GN=PEA2 PE=1 SV=1 [Swiss-Prot] PEA2 Protein involved in oriented growth toward mating partner [mips] Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth [SGD] PEA2, Chr V from 466203-467465, reverse complement [yeast_orfs2] Chr V from 466203-467465, reverse complement [yeastorf3.fasta] Chr V from 467465-466203, reverse complement [yeastorf5.fasta] (P40091) PEA2 protein (PPF2 protein) [4932.FASTACCERV.txt] PEA2 SGDID:S0000951, Chr V from 467465-466203, reverse complement, Verified ORF [yeastflysequest.fasta] Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth [Saccharomyces cerevisiae]gi\|1502362\|emb\|CAA6886 [nrdb_11152004_Chlamydomonas] PEA2 SGDID:S000000951, Chr V from 467465-466203, reverse complement, Verified ORF, "Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; loca [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Pea2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Pea2 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ppf2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth; Pea2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein PEA2 (Protein PPF2) [nr-271106-contam.fasta] PEA2 SGDID:S000000951, Chr V from 467465-466203, reverse complement, Verified ORF, "Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Protein PEA2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PEA2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PEA2 SGDID:S000000951, Chr V from 467470-466208, Genome Release 64-1-1, reverse complement, Verified ORF, "Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth" [yeast-030211-contam.fasta] PEA2 SGDID:S000000951, Chr V from 467470-466208, Genome Release 64-2-1, reverse complement, Verified ORF, "Coiled-coil 12S polarisome subunit; required for polarity establishment, apical bud growth, shmoo formation, filamentous differentiation; involved in Bni1p localization at sites of polarized growth, controlling polarized assembly of actin cables; role in apical growth affects diploid-specific bipolar bud site selection; retains Slt2p at bud tip to regulate ER inheritance; role in Ca2+ influx, cell fusion; S288C allele encoding Leu409 rather than Met linked with non-invasion" [20141208_orf_trans.fasta] YER149C SGDID:S000000951, Chr V from 467470-466208, Genome Release 64-1-1, reverse complement, Verified ORF, "Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	420 amino acids
Gene Ontology:
Cellular Component:	polarisome [TAS] actin cap [IDA]
Biological Process:	filamentous growth [IMP] bipolar cellular bud site selection [TAS] regulation of initiation of mating projection growth [IMP] pseudohyphal growth [IMP] actin filament organization [IPI] regulation of termination of mating projection growth [IMP] Rho protein signal transduction [IPI]
Molecular Function:	cytoskeletal regulatory protein binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MHKFDLELSR RANPLLFSAE RYEEYPLKYD ELKQYLLSQN PSHPHHNSRP YTSIDYFDYL  60
   61 LYRSKNDESE IDLDKKLVSE FALYYVQKEH LNSDDLNPTL NELLKLQPKS ADWYEMMLRI 120
  121 LESINTTGID QLTKENNNSF PNSKRARSST NMGGTDKFNK GAYHTDKADD DKNEILQELT 180
  181 SFLMSNSIQK GIDIKPIPLD DPVKFLKNGI NSILDTCVSL EKNTSSPPIS PNAAAIQEED 240
  241 SSKKLEELET AFSDLQLAHN FLTKQFENDR AEYVQDIEKL TRTNRELQDK LLNNHSNLSK 300
  301 TEKKLHELEQ ENKELEKANN KLNSSRHNFG MSSPASSPVT WDPSSPSSVG SPTSGSGSRS 360
  361 LSIMTSEFKK VLTSTQRKYE KELSDEREHR FKLERELALL KNAEANTSLA LNRDDPPDML 420
  421

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		PEA2 SPA2
View Details	Krogan NJ, et al. (2006)		BEM2 PEA2 SPA2
View Details	Qiu et al. (2008)		BEM1 BNI1 BNR1 BOI1 BOI2 BUD6 CAP1 CDC42 CLA4 CRN1 END3 GIC1 GIC2 HOF1 HSK3 IQG1 LAS17 MLC1 MYO2 PAN1 PEA2 PFY1 PXL1 SLG1 SPA2 SPH1 TPM2 VRP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	PEA2	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..137]	ORFEUS	7.29	Alpha chain
2	View Details	[138..420]	ORFEUS	7.29	Alpha chain

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: PEA2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: